CAJ | 학술논문

目的探讨载脂蛋白C3(APOC3)对THP-1细胞与小鼠主动脉黏附效应的影响.方法在无菌环境下应用外科显微技术分离C57BL/6小鼠主动脉,在体外给予APOC3刺激16 h后与标记有CFSE荧光的单核细胞来源THP-1细胞黏附1h,观察黏附效应的变化,同时应用免疫组化方法检测血管黏附分子1(VCAM-1)和细胞间黏附分子1(ICAM-1)的表达.结果给予APOC3刺激的主动脉与对照主动脉相比,与THP-1细胞的黏附效应增加,VCAM-1和ICAM-1表达上调,且前者比后者上调显著.结论载脂蛋白C3促进THP-1细胞向小鼠主动脉黏附.
목적 탐토재지단백C3(APOC3)대THP-1세포여소서주동맥점부효응적영향.방법 재무균배경하응용외과현미기술분리C57BL/6소서주동맥,재체외급여APOC3자격16 h후여표기유CFSE형광적단핵세포래원THP-1세포점부1h,관찰점부효응적변화,동시응용면역조화방법검측혈관점부분자1(VCAM-1)화세포간점부분자1(ICAM-1)적표체.결과 급여APOC3자격적주동맥여대조주동맥상비,여THP-1세포적점부효응증가,VCAM-1화ICAM-1표체상조,차전자비후자상조현저.결론 재지단백C3촉진THP-1세포향소서주동맥점부.
Objective To investigate the enhancing effect of apolipoprotein C3 (APOC3) on THP-1 cell adhesion to aortas of mice.Methods Microsurgery was performed to separate the aorta of C57BL/6 mice in sterile condition.After stimulated by APOC3 (100 △g/ml) in vitro for 16 h,the aorta was allowed to adhere for 1 h with CFSE labeled THP-1 cells (1 ×106/ml).Then the adhesion effect was observed,and the expressions of vascular adhesion molecule 1 (VCAM-1) and intercellular adhesion molecule 1 (ICAM-1) were detected by immunohistochemical method.Results Adhesion effect of the mice aorta with THP-1 cells in the APOC3 stimulated group was stronger than the control group.Both the expressions of VCAM-1 and ICAM-1 in aortas were increased by APOC3,but the former was significantly up-regulated than the latter.Conclusion Apolipoprotein C3 could enhance THP-1 cell adhesion to aortas of mice.