中华微生物学和免疫学杂志
中華微生物學和免疫學雜誌
중화미생물학화면역학잡지
CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
2013年
2期
129-132
,共4页
黄秋香%魏文进%郑海发%王三龙%刘雨%王春雨%钟汉斌%孟红彦%黎武军
黃鞦香%魏文進%鄭海髮%王三龍%劉雨%王春雨%鐘漢斌%孟紅彥%黎武軍
황추향%위문진%정해발%왕삼룡%류우%왕춘우%종한빈%맹홍언%려무군
甲型肝炎灭活疫苗%食蟹猴%免疫原性
甲型肝炎滅活疫苗%食蟹猴%免疫原性
갑형간염멸활역묘%식해후%면역원성
Inactived hepatitis A vaccine%Cynomolgus macaques%Immunogenicity
目的 研究甲型肝炎灭活疫苗(SH株)食蟹猴的免疫原性.方法 甲型肝炎病毒SH株接种人胚肺二倍体细胞(MRC-5),培养、收获的病毒液,经纯化、灭活后铝佐剂吸附制成甲型肝炎灭活疫苗.采用食蟹猴进行疫苗的免疫原性研究.结果 接种疫苗后的食蟹猴,体内产生抗HAV抗体和中和抗体,抗核抗体结果为阴性.结论该疫苗具有良好的免疫原性.
目的 研究甲型肝炎滅活疫苗(SH株)食蟹猴的免疫原性.方法 甲型肝炎病毒SH株接種人胚肺二倍體細胞(MRC-5),培養、收穫的病毒液,經純化、滅活後鋁佐劑吸附製成甲型肝炎滅活疫苗.採用食蟹猴進行疫苗的免疫原性研究.結果 接種疫苗後的食蟹猴,體內產生抗HAV抗體和中和抗體,抗覈抗體結果為陰性.結論該疫苗具有良好的免疫原性.
목적 연구갑형간염멸활역묘(SH주)식해후적면역원성.방법 갑형간염병독SH주접충인배폐이배체세포(MRC-5),배양、수획적병독액,경순화、멸활후려좌제흡부제성갑형간염멸활역묘.채용식해후진행역묘적면역원성연구.결과 접충역묘후적식해후,체내산생항HAV항체화중화항체,항핵항체결과위음성.결론해역묘구유량호적면역원성.
Objective To study on the immunogenicity of inactived hepatitis A vaccine in cynomolgus macaques.Methods The SH strain of hepatitis A virus was cultured in human embryo lung diploid cell (MRC-5) to harvest virus bulk,which was absorbed with alum adjuvant to prepare inactived hepatitis A vaccine after purification and inactivation.Then the study on the immunogenicity of vaccine was carried out on cynomolgus macaques.Results Specific anti-HAV antibodies and neutralizing antibodies were induced in cynomolgus macaques after immunization,while the test result of antinuclear antibodies appeared negative.Conclusion The inactivated hepatitis A vaccine prepared with SH strain showed high safety and good immunogenicity.
