CAJ | 학술논문

目的揭示广东地区2009-2011年新型H1N1病毒血凝素基因(HA)进化特征及抗原表位变异特征.方法采用时空抽样方法抽样,检测2009-2011年广东分离的24株新型H1N1病毒HA基因核苷酸序列,与GenBank中44株国外相应序列比较；比对HA基因核苷酸序列,分析基因分子变异,构建分子遗传动力学MCMC进化树；同时分析2009-2011年广东毒株HA基因的抗原表位变异情况.结果 68株HA基因进化树显示,广东毒株进化树主干至少分为6大分支；其中2011年毒株聚类为2个(Ⅴ和Ⅵ)主要分支,各具基因特征.变异频率较高的位点包括391、467、202和214位,正向选择位点包括8、145和391；广东毒株抗原表位区发生S145L/P、L208I、Q240R、S160G和G187R位点变异.2009年广东毒株HA基因分支Ⅰ毒株可能于2010年传播到亚洲、欧洲和澳洲地区.结论广东新型H1N1病毒HA基因变异具有传播特征(Ⅰ)和地区特征(Ⅵ),位于抗原表位Ca、Sa和Sb的氨基酸发生变异,其中位点145等变异频率较高,承受着正向选择压力.
목적 게시엄동지구2009-2011년신형H1N1병독혈응소기인(HA)진화특정급항원표위변이특정.방법 채용시공추양방법추양,검측2009-2011년엄동분리적24주신형H1N1병독HA기인핵감산서렬,여GenBank중44주국외상응서렬비교；비대HA기인핵감산서렬,분석기인분자변이,구건분자유전동역학MCMC진화수；동시분석2009-2011년엄동독주HA기인적항원표위변이정황.결과 68주HA기인진화수현시,엄동독주진화수주간지소분위6대분지；기중2011년독주취류위2개(Ⅴ화Ⅵ)주요분지,각구기인특정.변이빈솔교고적위점포괄391、467、202화214위,정향선택위점포괄8、145화391；엄동독주항원표위구발생S145L/P、L208I、Q240R、S160G화G187R위점변이.2009년엄동독주HA기인분지Ⅰ독주가능우2010년전파도아주、구주화오주지구.결론 엄동신형H1N1병독HA기인변이구유전파특정(Ⅰ)화지구특정(Ⅵ),위우항원표위Ca、Sa화Sb적안기산발생변이,기중위점145등변이빈솔교고,승수착정향선택압력.
Objective To reveal hemagglutinin gene (HA) characteristics and variability of Guangdong H1N1 pandemic viruses (H1Nlpdm) during 2009-2011.Methods Using time-space sampling,the nucleotide sequences of twenty-four HA genes of Guangdong H1Nlpdm influenza viruses isolated between 2009-2011 were sequenced and compared with forty-four HA sequences in GenBank.The mutations and antigenic variation were analyzed and phylodynamics MCMC tree was established.Results Phylogenetic tree of all 68 HA genes was divided into at least 6 branches.In 2011,Guangdong H1N1 strains clustered into 2 major clades (Ⅴ and Ⅵ) with local strain characteristics.The amino acid sites 391,467,202 and 214 were highly variable while the positive selective sites located at 8,145 and 391 aa.The epitope mutations of HA genes among Guangdong strains occurred in S145L/P,L2081,Q240R,S160G and G187R.Guangdong Ⅰ genotyping emerged in 2009 might be spread to Asia,Europe and Australia in 2010.Conclusion Guangdong H1N1pdm virus HA gene mutations showed propagation (Ⅰ) and regional(Ⅵ) characteristics.The mutations occurred in epitope Ca,Sa and Sb particularly with high variable rate in 145 aa and other sites indicated the existence of the positive immunological pressure.