中华微生物学和免疫学杂志
中華微生物學和免疫學雜誌
중화미생물학화면역학잡지
CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
2013年
3期
178-183
,共6页
付建光%嵇红%单军%王慎骄%李志锋%靳淼%刘成%秦圆方%田华
付建光%嵇紅%單軍%王慎驕%李誌鋒%靳淼%劉成%秦圓方%田華
부건광%혜홍%단군%왕신교%리지봉%근묘%류성%진원방%전화
诺如病毒%分子特征%GⅡ.4-2010亚型%重组
諾如病毒%分子特徵%GⅡ.4-2010亞型%重組
낙여병독%분자특정%GⅡ.4-2010아형%중조
Norovirus%Molecular characteristics%G Ⅱ.4-2010 subtype%Recombinant
目的 了解苏州地区诺如病毒的感染状况及其变异株的分子特征.方法 收集苏州市儿童医院疑似病毒性腹泻粪便标本419例,采用荧光定量PCR方法检测粪便中诺如病毒RNA及其基因组别,并对部分阳性标本测序分析.结果 419份粪便标本中,GⅡ组诺如病毒为122例,阳性率为29％,未检测出GⅠ组诺如病毒,对本地区部分诺如病毒阳性标本进行测序,A区(RdRp区)测序的25份GⅡ标本中25株均为GⅡ.4型；C区(N-S区)测序的26份GⅡ标本中17株为GⅡ.4型,8株为GⅡ.3型,1株为GⅡ.2型；D区(P区)测序的25份GⅡ标本中16株为GⅡ.4型,9株为GⅡ.3型.结论 诺如病毒是本地区婴幼儿病毒性腹泻的重要病原体,以诺如病毒GⅡ.4-2006b亚型为主,此外发现有GⅡ.4-2010亚型(GⅡ.4 New Orleans株)的流行,这是国内首次对该亚型毒株进行报道,同时还检测到部分重组毒株,提示诺如病毒在本地区的遗传变异日趋复杂.
目的 瞭解囌州地區諾如病毒的感染狀況及其變異株的分子特徵.方法 收集囌州市兒童醫院疑似病毒性腹瀉糞便標本419例,採用熒光定量PCR方法檢測糞便中諾如病毒RNA及其基因組彆,併對部分暘性標本測序分析.結果 419份糞便標本中,GⅡ組諾如病毒為122例,暘性率為29％,未檢測齣GⅠ組諾如病毒,對本地區部分諾如病毒暘性標本進行測序,A區(RdRp區)測序的25份GⅡ標本中25株均為GⅡ.4型；C區(N-S區)測序的26份GⅡ標本中17株為GⅡ.4型,8株為GⅡ.3型,1株為GⅡ.2型；D區(P區)測序的25份GⅡ標本中16株為GⅡ.4型,9株為GⅡ.3型.結論 諾如病毒是本地區嬰幼兒病毒性腹瀉的重要病原體,以諾如病毒GⅡ.4-2006b亞型為主,此外髮現有GⅡ.4-2010亞型(GⅡ.4 New Orleans株)的流行,這是國內首次對該亞型毒株進行報道,同時還檢測到部分重組毒株,提示諾如病毒在本地區的遺傳變異日趨複雜.
목적 료해소주지구낙여병독적감염상황급기변이주적분자특정.방법 수집소주시인동의원의사병독성복사분편표본419례,채용형광정량PCR방법검측분편중낙여병독RNA급기기인조별,병대부분양성표본측서분석.결과 419빈분편표본중,GⅡ조낙여병독위122례,양성솔위29％,미검측출GⅠ조낙여병독,대본지구부분낙여병독양성표본진행측서,A구(RdRp구)측서적25빈GⅡ표본중25주균위GⅡ.4형；C구(N-S구)측서적26빈GⅡ표본중17주위GⅡ.4형,8주위GⅡ.3형,1주위GⅡ.2형；D구(P구)측서적25빈GⅡ표본중16주위GⅡ.4형,9주위GⅡ.3형.결론 낙여병독시본지구영유인병독성복사적중요병원체,이낙여병독GⅡ.4-2006b아형위주,차외발현유GⅡ.4-2010아형(GⅡ.4 New Orleans주)적류행,저시국내수차대해아형독주진행보도,동시환검측도부분중조독주,제시낙여병독재본지구적유전변이일추복잡.
Objective To investigate norovirus infection status and indentify molecular characteristics of its variants in Suzhou.Methods Four hundred and ninteen fecal specimens of infantile viral diarrhea cases were collected from Suzhou Children's Hospital in 2011.Norovirus genomes were detected by Real-Time RT-PCR,and genotypes were determined by sequence analysis.Results Among all fecal specimens,122(29％) cases were positive for norovirus G Ⅱ,and G Ⅰ was not found.Nucleotide sequence analysis of genome regions A(RdRp region),C(N-S region) and D(P region) were performed and genotypes were determined,respectively.Based on region A,25 strains were G Ⅱ.4 in the 25 samples.Based on region C among 26 samples,17 strains with G Ⅱ.4,8 strains with G Ⅱ.3 and 1 strain with G Ⅱ.2 were identified,respectively.16 strains were G Ⅱ.4 and 9 strains were G Ⅱ.3 in 25 samples based on region D characteristics.As the most important pathogens causing infantile viral diarrhea in Suzhou,type G Ⅱ.4 (mainly G Ⅱ.4-2006b subtype) viruses were the dominant strain of norovirus.In addition,G Ⅱ.4-2010subtype viruses (G Ⅱ.4 New Orleans strain) were also detected.Conclusion To our knowledge,this is the first time reporting the detection of G Ⅱ.4-2010 viruses in China.A variety of recombinant viral strains were detected,suggesting that the complexity of the genetic variation of norovirus were increasing in Suzhou.
