中华微生物学和免疫学杂志
中華微生物學和免疫學雜誌
중화미생물학화면역학잡지
CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
2013年
3期
212-215
,共4页
刘瑞敏%孙颖川%张爱红%朱诗白%白慧玲%马远方
劉瑞敏%孫穎川%張愛紅%硃詩白%白慧玲%馬遠方
류서민%손영천%장애홍%주시백%백혜령%마원방
类风湿关节炎%TIPE2%自身免疫性疾病%CIA小鼠模型
類風濕關節炎%TIPE2%自身免疫性疾病%CIA小鼠模型
류풍습관절염%TIPE2%자신면역성질병%CIA소서모형
Rheumatoid arthritis%TIPE2%Autoimmune disease%Collagen induced arthritis model
目的 探讨TIPE2 (tumor necrosis factor-a induced protein 8 like 2)在牛Ⅱ型胶原诱导的CIA模型小鼠脾脏及类风湿关节炎(rheumatoid arthritis,RA)患者的外周血淋巴细胞中的表达及其意义.方法 使用牛Ⅱ型胶原免疫DBA-1/j小鼠,建立CIA小鼠模型;半定量RT-PCR法检测CIA小鼠脾脏中TIPE2基因的表达变化,荧光定量RT-PCR检测患者和健康对照者新鲜分离的淋巴细胞中TIPE2基因的表达差异;Western blot检测CIA小鼠脾脏及患者淋巴细胞中TIPE2蛋白表达差异.并研究TIPE2与类风湿关节炎患者病情活动程度的关系.结果 CIA小鼠的脾脏细胞中和患者外周血淋巴细胞中TIPE2在mRNA和蛋白水平表达增强,此外类风湿患者在活动期TIPE2表达更高,差异有统计学意义.类风湿关节炎患者的TIPE2表达水平和DAS28评分成明显正相关(P<0.001).结论 TIPE2参与了类风湿关节炎的发病过程,TIPE2可能是评价类风湿关节炎患者病情严重程度的标志物.
目的 探討TIPE2 (tumor necrosis factor-a induced protein 8 like 2)在牛Ⅱ型膠原誘導的CIA模型小鼠脾髒及類風濕關節炎(rheumatoid arthritis,RA)患者的外週血淋巴細胞中的錶達及其意義.方法 使用牛Ⅱ型膠原免疫DBA-1/j小鼠,建立CIA小鼠模型;半定量RT-PCR法檢測CIA小鼠脾髒中TIPE2基因的錶達變化,熒光定量RT-PCR檢測患者和健康對照者新鮮分離的淋巴細胞中TIPE2基因的錶達差異;Western blot檢測CIA小鼠脾髒及患者淋巴細胞中TIPE2蛋白錶達差異.併研究TIPE2與類風濕關節炎患者病情活動程度的關繫.結果 CIA小鼠的脾髒細胞中和患者外週血淋巴細胞中TIPE2在mRNA和蛋白水平錶達增彊,此外類風濕患者在活動期TIPE2錶達更高,差異有統計學意義.類風濕關節炎患者的TIPE2錶達水平和DAS28評分成明顯正相關(P<0.001).結論 TIPE2參與瞭類風濕關節炎的髮病過程,TIPE2可能是評價類風濕關節炎患者病情嚴重程度的標誌物.
목적 탐토TIPE2 (tumor necrosis factor-a induced protein 8 like 2)재우Ⅱ형효원유도적CIA모형소서비장급류풍습관절염(rheumatoid arthritis,RA)환자적외주혈림파세포중적표체급기의의.방법 사용우Ⅱ형효원면역DBA-1/j소서,건립CIA소서모형;반정량RT-PCR법검측CIA소서비장중TIPE2기인적표체변화,형광정량RT-PCR검측환자화건강대조자신선분리적림파세포중TIPE2기인적표체차이;Western blot검측CIA소서비장급환자림파세포중TIPE2단백표체차이.병연구TIPE2여류풍습관절염환자병정활동정도적관계.결과 CIA소서적비장세포중화환자외주혈림파세포중TIPE2재mRNA화단백수평표체증강,차외류풍습환자재활동기TIPE2표체경고,차이유통계학의의.류풍습관절염환자적TIPE2표체수평화DAS28평분성명현정상관(P<0.001).결론 TIPE2삼여료류풍습관절염적발병과정,TIPE2가능시평개류풍습관절염환자병정엄중정도적표지물.
Objective To investigate the expression of TIPE2 in splenic lymphocytes derived from the mouse model of cattle collagen Ⅱ-induced rheumatoid arthritis (CIA) and from peripheral blood of patients with rheumatoid arthritis (RA) for its role in the pathogenesis of RA.Methods The CIA mouse model was established by immunization of DBA-1/J mice with cattle collagen Ⅱ.Peripheral blood lymphocytes were collected from RA patients and healthy donors.The TIPE2 mRNA and protein expression in splenocytes of CIA and control mice were measured by semi-quantitative RT-PCR and Western blot,respectively.In addition,fluorescence quantitative RT-PCR and Western blot were used to determine the TIPE2 mRNA and protein expression in peripheral blood lymphocytes derived from patients with RA and healthy donors.Results Both mRNA and protein expression of TIPE2 were higher in splenocytes of CIA mice and lymphocytes of peripheral blood from RA patients compared with corresponding controls.Furthermore,the mRNA and protein expression of TIPE2 increased significantly in patients with active RA.TIPE2 expression were positively correlated with DAS28 scores (P<0.001).Conclusion Our results suggest that TIPE2 plays a role in the RA pathogenesis.The level of TIPE2 may also indicate the severity of rheumatoid arthritis.