CAJ | 학술논문

目的了解深圳地区感染性腹泻中星状病毒的感染情况,并研究其分子流行病学特点.方法采集深圳市2010年1月至2011年12月2083例疑似腹泻患者粪便标本,同时采集30例健康人粪便标本作为对照,采用荧光RT-PCR检测星状病毒核酸；然后使用特异性引物,对星状病毒核酸阳性标本进一步采用RT-PCR方法扩增星状病毒的ORF2区域,对PCR扩增产物经纯化、测序后进行序列分析,同时对病例的流行病学资料进行统计分析.结果星状病毒检出率为1.39％ (29/2083)；其中6月龄以下、6～11月龄、12 ～ 35月龄和36月龄以上患者检出率分别为2.96％ (5/169)、1.48％ (8/707)、2.48％(3/121)和1.20％(13/1086)；星状病毒在冬季检出率较高,春季次之,夏季和秋季则较少发生.对ORF2区域进行基因测序和分析显示,深圳地区星状病毒以HAstV-1为主要流行株,HAstV-2、HAstV-5亦有检出,其他型别则未检出.其中HAstV-1又可分为3个亚组,分别与GenBank上的德国株(AY720892)、印度株(AB551380)和巴西株(AY846638)有较高的亲缘关系,其核苷酸同源性分别为98.3％～99.1％、96.0％～ 100％和98.0％～ 100％.结论星状病毒是深圳地区病毒性腹泻的重要病原之一,HAstV-1是深圳地区星状病毒的主要流行株,应加强对星状病毒腹泻的监测.
목적 료해심수지구감염성복사중성상병독적감염정황,병연구기분자류행병학특점.방법 채집심수시2010년1월지2011년12월2083례의사복사환자분편표본,동시채집30례건강인분편표본작위대조,채용형광RT-PCR검측성상병독핵산；연후사용특이성인물,대성상병독핵산양성표본진일보채용RT-PCR방법확증성상병독적ORF2구역,대PCR확증산물경순화、측서후진행서렬분석,동시대병례적류행병학자료진행통계분석.결과 성상병독검출솔위1.39％ (29/2083)；기중6월령이하、6～11월령、12 ～ 35월령화36월령이상환자검출솔분별위2.96％ (5/169)、1.48％ (8/707)、2.48％(3/121)화1.20％(13/1086)；성상병독재동계검출솔교고,춘계차지,하계화추계칙교소발생.대ORF2구역진행기인측서화분석현시,심수지구성상병독이HAstV-1위주요류행주,HAstV-2、HAstV-5역유검출,기타형별칙미검출.기중HAstV-1우가분위3개아조,분별여GenBank상적덕국주(AY720892)、인도주(AB551380)화파서주(AY846638)유교고적친연관계,기핵감산동원성분별위98.3％～99.1％、96.0％～ 100％화98.0％～ 100％.결론 성상병독시심수지구병독성복사적중요병원지일,HAstV-1시심수지구성상병독적주요류행주,응가강대성상병독복사적감측.
Objective To investigate Astrovirus infection status and indentify its molecular characteristics in Shenzhen.Methods During January 2010 to December 2011,2083 stool samples were collected from patients with suspected diarrhea and 30 stool samples were collected from healthy people in Shenzhen.The nucleic acids extracted from the samples were detected by real-time polymerase chain reaction (real-time PCR).The sequences of ORF2 of the Astrovirus positive samples were amplified by RT-PCR,and then the products were sequenced after purification.The phylogenetic tree was constructed to analyze the nucleotide sequence homology between those sequences and the sequences of reference strains available in the GenBank database.Results 29 samples of 2083 were Astrovirus positive (1.39％,29/2083).The positive rates for patients under 6 months,6-11 months,12-35 months and above 36 months were 2.96％ (5/169),1.48％ (8/707),2.48％ (3/121) and 1.20％ (13/1086) respectively.The Astrovirus infection was commonly found in winter,followed by spring,but seldom occurred in summer and autumn.According to the analysis on the gene sequencing,strains of HAstV-l,HAstV-2 and HAstV-5 were detected,of which HAstV-1 was the predominant strain.The HAstV-1 strains could further be divided into three subtypes,which had high phylogenetic relationships with Dresden of Germany (AY720892),MASTO2392 of India (AB551380),and 1165 of Brazil (AY846638) in GenBank,with a nucleotide sequence homology of 98.3％-99.1％,96.0％-100％ and 98.0％-100％ respectively.Conclusion HAstV was one of the important pathogens causing viral diarrhea in Shenzhen and HAstV-1 was the predominant strain.Therefore,it is necessary to strengthen the surveillance for diarrhea caused by Astrovirus infection.