CAJ | 학술논문

目的研究白细胞介素(IL)-1A和IL-1B基因多态性及其与结核易感性之间的关系.方法应用PCR-时间飞行质谱技术(PCR-MassARRAY)分析1032例结核病患者和1008例非结核病患者的IL-1A和IL-1B基因多态性,并通过统计学分析IL-1A和IL-1B各单核苷酸多态性(SNP)位点基因型与活动性结核病之间的关系.结果在筛选出的5个IL-1A和IL-1B标签SNP位点中,rs2853550和rs3783526的等位基因频率在结核组和对照组间存在分布差异(分别为P=0.047和P=0.034).IL-1B SNP1 rs2853550 (P=0.025,OR=1.302,95％ CI=1.034～ 1.640,TC vs.CC)具有显著的结核风险,而其他两个IL-1B位点及IL-1A的2个SNP位点则与结核发病无显著关联.在Akaike 信息标准(AIC)定义的显性遗传模型下,IL-1B SNP1 rs2853550 [P=0.019,OR=1.308,95％ CI=1.045～1.638,(TC+TT) vs.CC]位点表现出显著的结核风险.而在隐性遗传模型下发现IL-1ASNP2 rs3783526[P=0.040,OR=0.764,95％ CI=0.591～0.988,GG vs.(AA+GA)]位点具有结核抗性,结核风险减少.在IL-1B基因的单体域中,“TG”相比频率最高的“CA”表现出显著的结核风险(P=0.032,OR=1.265,95％ CI=1.020～1.567).IL-1A内单体型中含“GA”的双单体型和IL-1B的单体型中含“TG”的双单体型,1份拷贝时,都表现出显著的结核风险(P=0.014,OR=1.403,95％ CI =1.072 ～1.836;P=0.013,OR=1.339,95％ CI=1.063～1.688).IL-1B的单体型中含“CG”的双单体型,在2份拷贝时,则带有结核抗性(P=0.006,OR=0.664,95％ CI=0.494～0.891).结论 IL1B rs2853550基因多态性可能与结核易感性密切相关,IL-1A rs3783526基因GG基因型可能具有结核抗性.
목적 연구백세포개소(IL)-1A화IL-1B기인다태성급기여결핵역감성지간적관계.방법 응용PCR-시간비행질보기술(PCR-MassARRAY)분석1032례결핵병환자화1008례비결핵병환자적IL-1A화IL-1B기인다태성,병통과통계학분석IL-1A화IL-1B각단핵감산다태성(SNP)위점기인형여활동성결핵병지간적관계.결과 재사선출적5개IL-1A화IL-1B표첨SNP위점중,rs2853550화rs3783526적등위기인빈솔재결핵조화대조조간존재분포차이(분별위P=0.047화P=0.034).IL-1B SNP1 rs2853550 (P=0.025,OR=1.302,95％ CI=1.034～ 1.640,TC vs.CC)구유현저적결핵풍험,이기타량개IL-1B위점급IL-1A적2개SNP위점칙여결핵발병무현저관련.재Akaike 신식표준(AIC)정의적현성유전모형하,IL-1B SNP1 rs2853550 [P=0.019,OR=1.308,95％ CI=1.045～1.638,(TC+TT) vs.CC]위점표현출현저적결핵풍험.이재은성유전모형하발현IL-1ASNP2 rs3783526[P=0.040,OR=0.764,95％ CI=0.591～0.988,GG vs.(AA+GA)]위점구유결핵항성,결핵풍험감소.재IL-1B기인적단체역중,“TG”상비빈솔최고적“CA”표현출현저적결핵풍험(P=0.032,OR=1.265,95％ CI=1.020～1.567).IL-1A내단체형중함“GA”적쌍단체형화IL-1B적단체형중함“TG”적쌍단체형,1빈고패시,도표현출현저적결핵풍험(P=0.014,OR=1.403,95％ CI =1.072 ～1.836;P=0.013,OR=1.339,95％ CI=1.063～1.688).IL-1B적단체형중함“CG”적쌍단체형,재2빈고패시,칙대유결핵항성(P=0.006,OR=0.664,95％ CI=0.494～0.891).결론 IL1B rs2853550기인다태성가능여결핵역감성밀절상관,IL-1A rs3783526기인GG기인형가능구유결핵항성.
Objective To study the correlation between genetic polymorphisms of interleukin (IL)-1A/1B and susceptibility to tuberculosis (TB).Methods Genetic polymorphisms of IL-1A and IL1 B in 1032 TB patients and 1008 non-TB patients were analyzed using PCR-MassARRAY method.The correlation between genetic polymorphisms of IL-1A/1B and susceptibility to TB was statistically analyzed.Results Two tag SNPs of IL-1A and three tag SNPs of IL-1B were screened for the study.There were differences in the allele frequencies of rs2853550 and rs3783526 between TB group and non-TB group (P=0.047and P =0.034,respectively).IL-1 B SNP1 rs2853550 (P =0.025,OR =1.302,95 ％ CI =1.034-1.640,TC vs.CC) was found to be highly associated with TB,while the other SNPs showed no significant correlations with TB.Furthermore,IL-1B SNP1 rs2853550 [P=0.019,OR=1.308,95％ CI=1.045-1.638 for (TC+TT) vs.CC] in the dominant model conferred significant risk for TB,but IL-1A SNP2 rs3783526 [P=0.000,OR=0.764,95％ CI =0.591-0.988 for GG vs.(AA+GA)] in the recessive model showed protective effects against TB.The haplotype ‘TG’ in the IL-1B block showed a higher risk for TB compared with the common ‘ CA’ haplotype (P=0.032,OR=1.265,95％ CI=1.020-1.567).The diplotypes containing ‘ GA’ haplotype in IL-1A block and ‘ TG’ haplotype in IL-1B block were major risk factors for TB (for onecopy,adjusted P=0.014,OR=1.403 and 95％ CI=1.072-1.836; adjusted P=0.013,OR=1.339 and 95％ CI=1.063-1.688,respectively),but the diplotype with ‘CG’ in IL-1B block played a protective effect against TB (for two-copy,P=0.006,OR=0.664 and95％ CI=0.494-0.891).Conclusion The genetic polymorphisms of IL-1B rs2853550 might be closely associated with TB,but the GG genotype of IL1 A SNP rs3783526 might have the characteristic of anti-TB.