中华微生物学和免疫学杂志
中華微生物學和免疫學雜誌
중화미생물학화면역학잡지
CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
2013年
5期
330-333
,共4页
朱传福%张毅%宋永红%刘艳%聂向民%乔文本%庄云龙
硃傳福%張毅%宋永紅%劉豔%聶嚮民%喬文本%莊雲龍
주전복%장의%송영홍%류염%섭향민%교문본%장운룡
人类白细胞抗原%等位基因分布%山东汉族
人類白細胞抗原%等位基因分佈%山東漢族
인류백세포항원%등위기인분포%산동한족
HLA%Allele distribution%Shandong Han population
目的 分析人类白细胞抗原(human leukocyte antigen,HLA)C等位基因在山东汉人群中的多态性分布.方法 对150名山东汉族无血缘关系的中华骨髓库志愿捐献者,采用聚合酶链反应-测序分型(polymerase chain reaction-sequence based typing,PCR-SBT)方法进行HLA-C基因分型,计数法计算各等位基因频率并采用x2比较与其他人群的差异.结果 观察到HLA-C等位基因25个,最常见等位基因为C*06∶02和C*07∶02,基因频率均超过10.00％,有16个等位基因频率＞1.00％,累计基因频率为95.33％.山东汉族人群HLA-C等位基因分布与中国北方汉族最为接近,与南方汉族有一定差异,与德国人和非洲裔美国人差异较大.结论 本文报道了山东汉族人群HLA-C等位基因的分布特点,对该地区HLA遗传学研究、疾病相关研究及移植配型等具有参考价值.
目的 分析人類白細胞抗原(human leukocyte antigen,HLA)C等位基因在山東漢人群中的多態性分佈.方法 對150名山東漢族無血緣關繫的中華骨髓庫誌願捐獻者,採用聚閤酶鏈反應-測序分型(polymerase chain reaction-sequence based typing,PCR-SBT)方法進行HLA-C基因分型,計數法計算各等位基因頻率併採用x2比較與其他人群的差異.結果 觀察到HLA-C等位基因25箇,最常見等位基因為C*06∶02和C*07∶02,基因頻率均超過10.00％,有16箇等位基因頻率＞1.00％,纍計基因頻率為95.33％.山東漢族人群HLA-C等位基因分佈與中國北方漢族最為接近,與南方漢族有一定差異,與德國人和非洲裔美國人差異較大.結論 本文報道瞭山東漢族人群HLA-C等位基因的分佈特點,對該地區HLA遺傳學研究、疾病相關研究及移植配型等具有參攷價值.
목적 분석인류백세포항원(human leukocyte antigen,HLA)C등위기인재산동한인군중적다태성분포.방법 대150명산동한족무혈연관계적중화골수고지원연헌자,채용취합매련반응-측서분형(polymerase chain reaction-sequence based typing,PCR-SBT)방법진행HLA-C기인분형,계수법계산각등위기인빈솔병채용x2비교여기타인군적차이.결과 관찰도HLA-C등위기인25개,최상견등위기인위C*06∶02화C*07∶02,기인빈솔균초과10.00％,유16개등위기인빈솔＞1.00％,루계기인빈솔위95.33％.산동한족인군HLA-C등위기인분포여중국북방한족최위접근,여남방한족유일정차이,여덕국인화비주예미국인차이교대.결론 본문보도료산동한족인군HLA-C등위기인적분포특점,대해지구HLA유전학연구、질병상관연구급이식배형등구유삼고개치.
Objective To analyze the distribution of HLA-C alleles in Shandong Han population of China.Methods One hundred and fifty unrelated potential donors,self-claimed as Han population from Shandong province,were selected from China Marrow Donor Program.Genotypes of HLA-C with the donors were identified by PCR-SBT.The frequencies of allele were calculated with direct counting method and the differences with other populations were analyzed with SPSS16.0 x2 software.Results A total of 25 alleles of HLA-C were observed and the most common alleles were C * 06:02 and C * 07:02 with the frequency of more than 10.00％.Moreover,there were 16 kinds of alleles with the frequency of more than 1.00％ accounting for 95.33％ of the total alleles.The distribution of HLA-C alleles in Shandong Han population was similar to that in northern Han population,but had some differences with that in southern Han population.In addition,the distribution of HLA-C alleles in Shandong Han population significantly differed from that of German/African American.Conclusion This study on the distribution of HLA-C alleles in Shandong Han population provides valuable references for further studies on the genetics of HLA,cross-match for organ transplantation and other genetic-associated diseases in this population.
