中华微生物学和免疫学杂志
中華微生物學和免疫學雜誌
중화미생물학화면역학잡지
CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
2013年
5期
339-345
,共7页
黄元兰%陈燕%孙懿%邓安梅%仲人前
黃元蘭%陳燕%孫懿%鄧安梅%仲人前
황원란%진연%손의%산안매%중인전
miR-146a%原发性胆汁性肝硬化%固有免疫%炎症因子
miR-146a%原髮性膽汁性肝硬化%固有免疫%炎癥因子
miR-146a%원발성담즙성간경화%고유면역%염증인자
miR-146a%Primary biliary cirrhosis%Innate immunity%Inflammatory factors
目的 分析原发性胆汁性肝硬化(PBC)患者单核细胞对内毒素(LPS)和多聚次黄嘌呤胞嘧啶核苷酸(Poly I∶C)刺激呈现出的高反应性是否与miR-146a有关.方法 以10 μg/ml的LPS或Poly I∶C分别刺激PBC患者和健康个体的外周血单核细胞,采用ELISA检测培养基内IL-1β、IL-6、TNF-α、IFN-α水平的变化,采用RT-PCR检测二者单核细胞内miR-146a、miR-21、let-7e、miR155、miR-125b表达变化情况.同时通过改变单核细胞系THP-1细胞株内miR-146a的表达来研究miR-146a在单核细胞对LPS和Poly I∶C刺激诱导活化中的调控作用.结果 LPS或Poly I∶C诱导PBC患者单核细胞释放炎症因子IL-1β、IL-6、TNF-α和IFN-α的能力显著强于健康个体单核细胞,但二者诱导PBC患者单核细胞表达miR-146a的能力弱于健康个体.在LPS或Poly I∶C的刺激下,改变THP-1细胞内miR-146a可以调节部分炎症因子的释放.结论 在接受到LPS或Poly I∶C刺激后,PBC患者单核细胞内miR-146a表达受损是导致单核细胞过度活化的原因之一.
目的 分析原髮性膽汁性肝硬化(PBC)患者單覈細胞對內毒素(LPS)和多聚次黃嘌呤胞嘧啶覈苷痠(Poly I∶C)刺激呈現齣的高反應性是否與miR-146a有關.方法 以10 μg/ml的LPS或Poly I∶C分彆刺激PBC患者和健康箇體的外週血單覈細胞,採用ELISA檢測培養基內IL-1β、IL-6、TNF-α、IFN-α水平的變化,採用RT-PCR檢測二者單覈細胞內miR-146a、miR-21、let-7e、miR155、miR-125b錶達變化情況.同時通過改變單覈細胞繫THP-1細胞株內miR-146a的錶達來研究miR-146a在單覈細胞對LPS和Poly I∶C刺激誘導活化中的調控作用.結果 LPS或Poly I∶C誘導PBC患者單覈細胞釋放炎癥因子IL-1β、IL-6、TNF-α和IFN-α的能力顯著彊于健康箇體單覈細胞,但二者誘導PBC患者單覈細胞錶達miR-146a的能力弱于健康箇體.在LPS或Poly I∶C的刺激下,改變THP-1細胞內miR-146a可以調節部分炎癥因子的釋放.結論 在接受到LPS或Poly I∶C刺激後,PBC患者單覈細胞內miR-146a錶達受損是導緻單覈細胞過度活化的原因之一.
목적 분석원발성담즙성간경화(PBC)환자단핵세포대내독소(LPS)화다취차황표령포밀정핵감산(Poly I∶C)자격정현출적고반응성시부여miR-146a유관.방법 이10 μg/ml적LPS혹Poly I∶C분별자격PBC환자화건강개체적외주혈단핵세포,채용ELISA검측배양기내IL-1β、IL-6、TNF-α、IFN-α수평적변화,채용RT-PCR검측이자단핵세포내miR-146a、miR-21、let-7e、miR155、miR-125b표체변화정황.동시통과개변단핵세포계THP-1세포주내miR-146a적표체래연구miR-146a재단핵세포대LPS화Poly I∶C자격유도활화중적조공작용.결과 LPS혹Poly I∶C유도PBC환자단핵세포석방염증인자IL-1β、IL-6、TNF-α화IFN-α적능력현저강우건강개체단핵세포,단이자유도PBC환자단핵세포표체miR-146a적능력약우건강개체.재LPS혹Poly I∶C적자격하,개변THP-1세포내miR-146a가이조절부분염증인자적석방.결론 재접수도LPS혹Poly I∶C자격후,PBC환자단핵세포내miR-146a표체수손시도치단핵세포과도활화적원인지일.
Objective To investigate whether the hyper-reactivity of monocytes from the patients with primary biliary cirrhosis (PBC) to LPS and Poly I ∶ C was associated with miR-146a.Methods Peripheral blood mononuclear cells from PBC patients and healthy controls were stimulated with 10 μg/ml of LPS or Poly I ∶ C.The levels of IL-1 β,IL-6,TNF-α and IFN-α in the culture medium were detected by ELISA.The relative expressions of miR-146a,miR-21,let-7e,miR-155 and miR-125b were detected by RT-PCR.The regulatory role of miR-146a on the production of inflammatory factors in LPS or Poly I ∶ C stimulated THP-1 cells was studied by "gain-and-loss" function assay.Results The up-regulation of miR146a,which was induced by both LPS or Poly I ∶ C,was impaired in the monocytes from PBC patients.The production of LPS or Poly I ∶ C induced inflammatory factor,could be enhanced by miR-146a down-regulation.Conclusion The hyper-reactivity of monocytes from PBC patients to LPS and Poly I ∶ C was associated with miR-146a.
