CAJ | 학술논문

目的了解广东省不同时期猩红热患儿酿脓链球菌的病原学特征.方法对不同时期广东省酿脓链球菌分离株22株进行emm分型、超抗原基因检测、抗生素敏感性分析和PFGE分子分型.结果酿脓链球菌对头孢噻肟、左旋氧氟沙星和青霉素100％敏感,2000年以后分离的酿脓链球菌对红霉素和克林霉素100％耐药,而2000年以前的菌株只有9.1％(1/11)对这两种药物耐药.22株酿脓链球菌有3个emm型:59.09％ (13/22)为emm12.0型,36.36％ (8/22)为emm6.0型,4.55％ (1/22)为emm1.0型.2011年和1997年分离的酿脓链球菌为emm12.0型,1978年和1986年分离的菌株为emm6.0型,2008年分离的菌株为emm1.0型.超抗原基因speA,speB,speC,speF,speG,speH,smeZ和ssa的检出率分别为:54.55％、100％、100％、100％、100％、54.55％、0％、86.36％,95.45％的菌株同时携带6个超抗原基因.22株酿脓链球菌可分为10种不同的PFGE型别,分成Ⅰ～Ⅲ3个聚集簇,聚集簇Ⅰ由1997年分离的菌株和1株2011年分离的菌株组成,聚集簇Ⅱ由1978年和1986年分离的菌株组成,聚集簇Ⅲ由9株2011年分离的菌株和1株2008年分离的菌株组成.结论广东省猩红热患儿酿脓链球菌对青霉素敏感,红霉素耐药,emm12.0型为主要emm型,酿脓链球菌的超抗原基因携带率高.
목적 료해광동성불동시기성홍열환인양농련구균적병원학특정.방법 대불동시기광동성양농련구균분리주22주진행emm분형、초항원기인검측、항생소민감성분석화PFGE분자분형.결과 양농련구균대두포새우、좌선양불사성화청매소100％민감,2000년이후분리적양농련구균대홍매소화극림매소100％내약,이2000년이전적균주지유9.1％(1/11)대저량충약물내약.22주양농련구균유3개emm형:59.09％ (13/22)위emm12.0형,36.36％ (8/22)위emm6.0형,4.55％ (1/22)위emm1.0형.2011년화1997년분리적양농련구균위emm12.0형,1978년화1986년분리적균주위emm6.0형,2008년분리적균주위emm1.0형.초항원기인speA,speB,speC,speF,speG,speH,smeZ화ssa적검출솔분별위:54.55％、100％、100％、100％、100％、54.55％、0％、86.36％,95.45％적균주동시휴대6개초항원기인.22주양농련구균가분위10충불동적PFGE형별,분성Ⅰ～Ⅲ3개취집족,취집족Ⅰ유1997년분리적균주화1주2011년분리적균주조성,취집족Ⅱ유1978년화1986년분리적균주조성,취집족Ⅲ유9주2011년분리적균주화1주2008년분리적균주조성.결론 광동성성홍열환인양농련구균대청매소민감,홍매소내약,emm12.0형위주요emm형,양농련구균적초항원기인휴대솔고.
Objective To investigate the etiological characteristics of Streptococcus pyogenes that caused scarlet fever in different periods in Guangdong province.Methods 22 isolates from different periods were analyzed through emm typing,PCR detection for super antigen genes,antibiotic susceptibility test and pulsed-field gel electrophoresis (PFGE).Results All isolates were susceptible to cefotaxim,levofloxacin and penicillin.Streptococcus pyogenes isolated after the year 2000 were 100％ resistant to erythrocin and clindamycin,but the resistant rate for strains isolated before the year 2000 was 9.1％ (1/11).There were 3 emm types indentified from 22 isolates including emm12.0 (59.09％,13/22),emm6.0 (36.36％,8/22) and emm1.0 (4.55％,1/22),which were detected in the isolates from the year 1997 and 2011,from 1978 and 1986,and from 2008,respectively.The positive rates for speA,speB,speC,speF,speG,speH,smeZ,and ssa genes detected by PCR were 54.55％,100％,100％,100％,100％,54.55％,0％,and 86.36％ respectively.Among all strains,95.45％ of the isolates carried 6 superantigen genes simultaneously.Three clusters of 10 PFGE subtypes were identified in 22 isolates.Cluster Ⅰ consisted of all strains from 1997 and one strain from 2011.Cluster Ⅱ consisted of strains isolated from 1978 and 1986.Cluster Ⅲ consisted of nine strains from 2011 and one from 2008.Conclusion S.pyrogenes isolates in Guangdong province were susceptible to penicillin but resistant to erythrocin.emm 12.0 accounted for the majority of the three types and there was a high frequency of super antigen genes.