中华微生物学和免疫学杂志
中華微生物學和免疫學雜誌
중화미생물학화면역학잡지
CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
2014年
1期
1-5
,共5页
郝金娟%刘真%任国良%赵全璧%胡园园%阮玉华%邵一鸣%洪坤学
郝金娟%劉真%任國良%趙全璧%鬍園園%阮玉華%邵一鳴%洪坤學
학금연%류진%임국량%조전벽%호완완%원옥화%소일명%홍곤학
人类免疫缺陷病毒%人类白细胞抗原%超型%病毒载量
人類免疫缺陷病毒%人類白細胞抗原%超型%病毒載量
인류면역결함병독%인류백세포항원%초형%병독재량
Human immunodeficiency virus%Human leukocyte antigen%Supertype%Viral load
目的 分析HIV-1 B'亚型感染者HLA-A03超型(HLA-A03s)和相关等位基因的分布及与病毒载量的关系.方法 采用聚合酶链反应-序列特异性引物(PCR-SSP)扩增和序列分析技术(SBT)对222例HIV感染者HLA-A位点等位基因进行分型并根据表位肽结合特异性进行超型归类,分析HLA-A03s和相关等位基因与病毒载量的相关性.结果 222名感染者中有146人(65.77％)至少携带一个HLA-A03s等位基因;携带HLA-A03s的感染者病毒载量中位数为17 900拷贝/ml,显著低于不携带该超型的感染者(35 760拷贝/ml,P=0.0005);携带HLA-A* 1101(Mann-Whitney U=8994,P=0.0096)或HLA-A* 3001(Mann-Whitney U=4048,P--0.0139)等位基因的感染者的病毒载量显著低于不携带者的病毒载量.结论 携带HLA-A03s、HLA-A*1101或HLA-A* 3001等位基因的感染者具有较低的病毒载量.
目的 分析HIV-1 B'亞型感染者HLA-A03超型(HLA-A03s)和相關等位基因的分佈及與病毒載量的關繫.方法 採用聚閤酶鏈反應-序列特異性引物(PCR-SSP)擴增和序列分析技術(SBT)對222例HIV感染者HLA-A位點等位基因進行分型併根據錶位肽結閤特異性進行超型歸類,分析HLA-A03s和相關等位基因與病毒載量的相關性.結果 222名感染者中有146人(65.77％)至少攜帶一箇HLA-A03s等位基因;攜帶HLA-A03s的感染者病毒載量中位數為17 900拷貝/ml,顯著低于不攜帶該超型的感染者(35 760拷貝/ml,P=0.0005);攜帶HLA-A* 1101(Mann-Whitney U=8994,P=0.0096)或HLA-A* 3001(Mann-Whitney U=4048,P--0.0139)等位基因的感染者的病毒載量顯著低于不攜帶者的病毒載量.結論 攜帶HLA-A03s、HLA-A*1101或HLA-A* 3001等位基因的感染者具有較低的病毒載量.
목적 분석HIV-1 B'아형감염자HLA-A03초형(HLA-A03s)화상관등위기인적분포급여병독재량적관계.방법 채용취합매련반응-서렬특이성인물(PCR-SSP)확증화서렬분석기술(SBT)대222례HIV감염자HLA-A위점등위기인진행분형병근거표위태결합특이성진행초형귀류,분석HLA-A03s화상관등위기인여병독재량적상관성.결과 222명감염자중유146인(65.77％)지소휴대일개HLA-A03s등위기인;휴대HLA-A03s적감염자병독재량중위수위17 900고패/ml,현저저우불휴대해초형적감염자(35 760고패/ml,P=0.0005);휴대HLA-A* 1101(Mann-Whitney U=8994,P=0.0096)혹HLA-A* 3001(Mann-Whitney U=4048,P--0.0139)등위기인적감염자적병독재량현저저우불휴대자적병독재량.결론 휴대HLA-A03s、HLA-A*1101혹HLA-A* 3001등위기인적감염자구유교저적병독재량.
Objective To investigate the frequencies of HLA-A03 supertype (HLA-A03s) alleles and their impact on HIV-1 viral load.Methods Polymerase chain reaction-sequence specific primers (PCR-SSP) and sequence-based typing assay (SBT) were used to genotype HLA alleles to four-digit resolution on 222 patients with HIV infection.Then the correlations between viral load and the frequencies of HLA-A03s and its corresponding alleles were analyzed.Results Of all 222 patients with HIV-1 B' infection,146 (65.77％) subjects carried at least one A03s allele.The median viral load of subjects with HLA-A03s was 17 900 copies/ml,which was significantly lower than that of subjects without HLA-A03s (35 760 copies/ml,P=0.0005).Subjects carrying HLA-A * 1101 (Mann-Whitney U=8994,P=0.0096) or HLA-A * 3001 (Mann-Whitney U=4048,P=0.0139) allele showed lower viral loads than those without the two alleles.Conclusion HLA-A03s,HLA-A * 1101 and HLA-A * 3001 alleles were associated with lower plasma viral loads in patients with HIV-1 B' infection.
