CAJ | 학술논문

目的建立一个稳定有效的大鼠皮肤感染模型,用于评价外用抗感染药物的疗效.方法成年SD大鼠皮下注射环磷酰胺建立稳定的免疫功能抑制状态后,在其背部行手术造创口,连续2d涂擦耐甲氧西林金黄色葡萄球菌菌液.肉眼观察伤口动态感染情况、伤口组织病理表现及伤口痊愈时间3项指标,作为判断建立皮肤感染模型的依据.结果人工感染的实验大鼠皮肤伤口出现典型化脓性感染,其中环磷酰胺处理组比未处理组大鼠的伤口感染情况和病理组织感染指征更重、伤口恢复速度明显减慢,痊愈时间延后大于10 d.结论在环磷酰胺免疫抑制SD大鼠中能成功建立皮肤外伤感染模型,可用于评价包括抗耐药菌株在内的外用抗感染药物的疗效.
목적 건립일개은정유효적대서피부감염모형,용우평개외용항감염약물적료효.방법 성년SD대서피하주사배린선알건립은정적면역공능억제상태후,재기배부행수술조창구,련속2d도찰내갑양서림금황색포도구균균액.육안관찰상구동태감염정황、상구조직병리표현급상구전유시간3항지표,작위판단건립피부감염모형적의거.결과 인공감염적실험대서피부상구출현전형화농성감염,기중배린선알처리조비미처리조대서적상구감염정황화병리조직감염지정경중、상구회복속도명현감만,전유시간연후대우10 d.결론 재배린선알면역억제SD대서중능성공건립피부외상감염모형,가용우평개포괄항내약균주재내적외용항감염약물적료효.
Objective To establish an effective and stable rat model of skin-infection for evaluating the therapeutic effects of topical anti-infection drugs.Methods SD rats were subcutaneously injected with cyclophosphamide to induce immunosuppression,and then surgical incisions were made on both sides of the spine.The rat model of skin-infection was established by applying methicillin-resistant Staphylococcus aureas suspension to the incisions for two consecutive days,and evaluated by analyzing infection status,pathological changes and healing time.Results The development of pyogenic infection was detected in all of the rats.Compared with the non-cyclophosphamide treated group,the cyclophosphamide treated group showed a more severe infection both from the visual inspection and the microscopic observation,moreover,its healing time was delayed more than 10 days.Conclusion The skin-infection model was successfully established in immunosuppressed rats induced by cyclophosphamide,which could be applicable to the efficacy evaluation of anti-infection drugs for external use on skin infection.