CAJ | 학술논문

目的探索IFN-γ对高效抗逆转录病毒治疗(HAART)1年的HIV/AIDS患者Treg细胞的调节作用.方法对30例CD4+T细胞数＜350个/μl的HIV/AIDS患者进行HAART治疗,并分别于HAART治疗前(0周)、治疗24周及48周备血,分离外周血单个核细胞(PBMC),随机分成2组进行无菌培养,一组直接培养,另一组加IFN-γ (40 pg/ml)培养,培养5d后分别收集上清液和细胞.ELISA检测上清液IL-12,流式细胞术检测CD4+CD25+ Foxp3 Treg细胞.结果 HAART治疗前、治疗24周、48周PBMC培养上清液中IL-12水平(单纯培养Vs共培养组)分别为:[(37.02±12.76)vs(41.79±15.02),t=2.336,P=0.03]、[(41.76±17.01) vs(47.2±14.26),t=2.702,P=0.014]、[(48.01±11.84)vs(53.44±11.30),t=3.14,P=0.003];培养细胞中CD4+CD25+Foxp3 Treg细胞占CD4+T细胞比例(单纯培养Vs共培养组)分别为:[(10.41±1.10)vs(2.40±1.11)％,t=13.89,p=0.000]、[(8.33±2.03) vs(1.99±0.86)％,t=12.93,P=0.000]、[(5.65±l.55)vs(1.32±0.73)％,t=10.61,P=0.000].且同组HAART治疗不同时间点间比较差异均有统计学意义(P＜0.05).结论随着HAART治疗时间的延长,HIV/AIDS患者IL-12水平逐渐升高,Treg细胞比例逐渐下降,IFN-γ起重要的免疫调节作用.
목적 탐색IFN-γ대고효항역전록병독치료(HAART)1년적HIV/AIDS환자Treg세포적조절작용.방법 대30례CD4+T세포수＜350개/μl적HIV/AIDS환자진행HAART치료,병분별우HAART치료전(0주)、치료24주급48주비혈,분리외주혈단개핵세포(PBMC),수궤분성2조진행무균배양,일조직접배양,령일조가IFN-γ (40 pg/ml)배양,배양5d후분별수집상청액화세포.ELISA검측상청액IL-12,류식세포술검측CD4+CD25+ Foxp3 Treg세포.결과 HAART치료전、치료24주、48주PBMC배양상청액중IL-12수평(단순배양Vs공배양조)분별위:[(37.02±12.76)vs(41.79±15.02),t=2.336,P=0.03]、[(41.76±17.01) vs(47.2±14.26),t=2.702,P=0.014]、[(48.01±11.84)vs(53.44±11.30),t=3.14,P=0.003];배양세포중CD4+CD25+Foxp3 Treg세포점CD4+T세포비례(단순배양Vs공배양조)분별위:[(10.41±1.10)vs(2.40±1.11)％,t=13.89,p=0.000]、[(8.33±2.03) vs(1.99±0.86)％,t=12.93,P=0.000]、[(5.65±l.55)vs(1.32±0.73)％,t=10.61,P=0.000].차동조HAART치료불동시간점간비교차이균유통계학의의(P＜0.05).결론 수착HAART치료시간적연장,HIV/AIDS환자IL-12수평축점승고,Treg세포비례축점하강,IFN-γ기중요적면역조절작용.
Objective To investigate the regulatory effects of IFN-γon Treg cells from HIV/AIDS patients receiving highly active antiretroviral therapy (HAART) for one year.Methods Thirty HIV/A1DS patients whose CD4+T cells were below 350/μ1 were recruited for HAART therapy.Blood samples were collected at the time points of 0,24,48 weeks after HAART.PBMCs were isolated and randomly divided into two culture groups.One group was cultured directly in medium and another group was co-cultured with IFN-γ (40 pg/ml).The supernatants and cells were separated after 5 days of culture for analysis.The concentrations of IL-12 and CD4+CD25+Foxp3 Treg cells were measured by ELISA and flow cytometry,respectively.Results The levels of IL-12 in the supernatants from the culture without IFN-γ at time points of 0,24,48 weeks after HAART were lower than those from the co-cultured group [(37.02±12.76) vs (41.79± 15.02),t=2.336,P=0.03; (41.76±17.01) vs (47.2±14.26),t=2.702,P=0.014; (48.01± 11.84) vs (53.44± 11.30),t =3.14,P =0.003].The percentages of CD4+ CD25 + Foxp3 Treg cells in CD4+ T cells from the direct-cultured group were higher than those from the co-cultured group at the three time points [(10.41±1.10)％ vs (2.40±1.11)％,t=13.89,P=0.000; (8.33±2.03)％ vs (1.99± 0.86)％,t=12.93,P=0.000; (5.65±1.55)％ vs (1.32±0.73)％,t=10.61,P=0.000].Moreover,the results within the same group at the time points of 0,24,48 weeks upon HAART were also significantly different.Conclusion With the interference of HAART,IL-12 levels were increased,while CD4+CD25+ Foxp3 Treg cells were decreased in patients with HIV/AIDS.IFN-γ plays an important role in this process.