CAJ | 학술논문

目的掌握真菌性角膜炎在吉林省的病原菌种类和流行病学特征,建立主要病原菌种的快速、特异性鉴定方法.方法采集225例疑似真菌性角膜炎患者的角膜刮片,分离、鉴定病原真菌,进行流行病学分析;建立主要病原菌种茄病镰刀菌的复合PCR鉴定方法,用于角膜刮片的快速鉴定.结果 225例患者中有156例确诊为真菌感染角膜炎,检出率为69.3％.其中12例患者为两种真菌共感染,共分离获得168株病原真菌.以镰刀菌属、曲霉属及假丝酵母属较多见,其中,主要病原菌种为茄病镰刀菌(34.5％).建立的复合PCR方法可根据330 bp特异性片段鉴定茄病镰刀菌,并可直接检测液体培养后的角膜刮片.结论真菌性角膜炎在吉林省的主要病原流行菌种为茄病镰刀菌.本文所建立的复合PCR方法适于角膜刮片标本的快速鉴定.
목적 장악진균성각막염재길림성적병원균충류화류행병학특정,건립주요병원균충적쾌속、특이성감정방법.방법 채집225례의사진균성각막염환자적각막괄편,분리、감정병원진균,진행류행병학분석;건립주요병원균충가병렴도균적복합PCR감정방법,용우각막괄편적쾌속감정.결과 225례환자중유156례학진위진균감염각막염,검출솔위69.3％.기중12례환자위량충진균공감염,공분리획득168주병원진균.이렴도균속、곡매속급가사효모속교다견,기중,주요병원균충위가병렴도균(34.5％).건립적복합PCR방법가근거330 bp특이성편단감정가병렴도균,병가직접검측액체배양후적각막괄편.결론 진균성각막염재길림성적주요병원류행균충위가병렴도균.본문소건립적복합PCR방법괄우각막괄편표본적쾌속감정.
Objective To investigate the etiological and epidemiological characteristics of fungal keratitis in Jilin province of China and to establish a rapid and specific method for molecular identification of the prevalent fungal pathogens.Methods Corneal scrapings were collected from 225 patients with suspected fungal keratitis.Fungal strains were isolated and identified based on their morphology and physiological characteristics.The epidemiological characteristics of all isolated strains causing fungal keratitis were statistically analyzed.Species-specific primers of Fusarium solani (F.solani) were designed and used together with the universal fungal primers to establish a multiplex PCR assay for identification of F.solani in corneal scrapings.Results 156 out of 225 patients (69.3％) were diagnosed as fungal keratitis by fungal culture followed by the examination of morphological and physiological characteristics.A total of 168 pathogenic fungi strains were isolated,most of which were Fasarium spp.(49.4％),followed by Aspergillus spp.(17.9％) and Candida spp.(14.3％).F.solani was the predominant pathogen accounting for 34.5％ in all patients.Most of the patients (87.5％) were farmer and male patients (57.1％) accounted for the majority of 156 patients as well.Corneal trauma (38.5％) was considered as the main predisposing factor.The established multiplex PCR could specifically amplify a 300 bp nucleotide fragment of F.solani.It could be used for a rapid identification of F.solani in corneal scrapings.Conclusion Fusarium genus,particularly the species of F.solani,was the predominant pathogen for fungal keratitis in Jilin province of China.Corneal trauma was the most important predisposing factor.The established multiplex PCR could identify fungal infection from corneal scrapings rapidly and specifically.These findings are very important for the early diagnosis and treatment of fungal keratitis.