中华消化内镜杂志
中華消化內鏡雜誌
중화소화내경잡지
CHINESE JOURNAL OF DIGESTIVE ENDOSCOPY
2009年
10期
527-532
,共6页
唐慧%邹云莲%朱军%严颜%寿涛%左赞%何旭%杨昆宪%严新民%郭强
唐慧%鄒雲蓮%硃軍%嚴顏%壽濤%左讚%何旭%楊昆憲%嚴新民%郭彊
당혜%추운련%주군%엄안%수도%좌찬%하욱%양곤헌%엄신민%곽강
腺瘤息肉病%结肠%腺癌%基因表达芯片%基因表达谱
腺瘤息肉病%結腸%腺癌%基因錶達芯片%基因錶達譜
선류식육병%결장%선암%기인표체심편%기인표체보
Adenomatous polyposis coli%Adenocarcinoma%Gene expression chips%Gene expression profiling
目的 筛选贯穿于大肠癌发生、发展全过程的基因,探讨大肠腺瘤-癌序列发生的分子机制.方法 Trizol法分别提取正常大肠黏膜,大肠腺瘤,Dukes A、Dukes B和Dukes C-D大肠腺癌组织的总RNA,分离纯化mRNA,逆转录得双链cDNA,生物素标记cRNA探针,与Affymetrix U133 PLAS2.0基因芯片(涵盖18400个转录本,代表14500个功能已知的基因)杂交,Gene Scanner3000激光系统扫描,GCOS 1.2分析软件读取处理杂交信号.计算机分析,比较5种组织基因表达谱差异.结果 分别以大肠腺瘤,Dukes A、Dukes B和Dukes C-D大肠腺癌组织与正常大肠黏膜组织的基因表达谱相比后发现在4个组中存在共同表达差异的基因253个,其中表达持续上调基因34个(已知基因29个,未知功能基因5个),表达持续下调基因219个(已知基因196个,未知功能基因23个).结论 大肠癌的发生发展是一个多基因参与的复杂演变过程.这些基因在大肠癌前病变-大肠腺瘤阶段就已经有异常表达,说明正常大肠黏膜细胞恶性转变的潜能在癌前病变阶段就已经存在,并且其作用持续贯穿于大肠癌发生发展的整个过程.因此,对这些基因的进一步研究有助于全面了解大肠腺瘤-癌序列的分子机制,并对大肠癌的早期诊断和及时干预治疗具有指导意义.
目的 篩選貫穿于大腸癌髮生、髮展全過程的基因,探討大腸腺瘤-癌序列髮生的分子機製.方法 Trizol法分彆提取正常大腸黏膜,大腸腺瘤,Dukes A、Dukes B和Dukes C-D大腸腺癌組織的總RNA,分離純化mRNA,逆轉錄得雙鏈cDNA,生物素標記cRNA探針,與Affymetrix U133 PLAS2.0基因芯片(涵蓋18400箇轉錄本,代錶14500箇功能已知的基因)雜交,Gene Scanner3000激光繫統掃描,GCOS 1.2分析軟件讀取處理雜交信號.計算機分析,比較5種組織基因錶達譜差異.結果 分彆以大腸腺瘤,Dukes A、Dukes B和Dukes C-D大腸腺癌組織與正常大腸黏膜組織的基因錶達譜相比後髮現在4箇組中存在共同錶達差異的基因253箇,其中錶達持續上調基因34箇(已知基因29箇,未知功能基因5箇),錶達持續下調基因219箇(已知基因196箇,未知功能基因23箇).結論 大腸癌的髮生髮展是一箇多基因參與的複雜縯變過程.這些基因在大腸癌前病變-大腸腺瘤階段就已經有異常錶達,說明正常大腸黏膜細胞噁性轉變的潛能在癌前病變階段就已經存在,併且其作用持續貫穿于大腸癌髮生髮展的整箇過程.因此,對這些基因的進一步研究有助于全麵瞭解大腸腺瘤-癌序列的分子機製,併對大腸癌的早期診斷和及時榦預治療具有指導意義.
목적 사선관천우대장암발생、발전전과정적기인,탐토대장선류-암서렬발생적분자궤제.방법 Trizol법분별제취정상대장점막,대장선류,Dukes A、Dukes B화Dukes C-D대장선암조직적총RNA,분리순화mRNA,역전록득쌍련cDNA,생물소표기cRNA탐침,여Affymetrix U133 PLAS2.0기인심편(함개18400개전록본,대표14500개공능이지적기인)잡교,Gene Scanner3000격광계통소묘,GCOS 1.2분석연건독취처리잡교신호.계산궤분석,비교5충조직기인표체보차이.결과 분별이대장선류,Dukes A、Dukes B화Dukes C-D대장선암조직여정상대장점막조직적기인표체보상비후발현재4개조중존재공동표체차이적기인253개,기중표체지속상조기인34개(이지기인29개,미지공능기인5개),표체지속하조기인219개(이지기인196개,미지공능기인23개).결론 대장암적발생발전시일개다기인삼여적복잡연변과정.저사기인재대장암전병변-대장선류계단취이경유이상표체,설명정상대장점막세포악성전변적잠능재암전병변계단취이경존재,병차기작용지속관천우대장암발생발전적정개과정.인차,대저사기인적진일보연구유조우전면료해대장선류-암서렬적분자궤제,병대대장암적조기진단화급시간예치료구유지도의의.
Objective To screen genes present in the whole process of colorectal tumor development,and to explore the molecular mechanism underlying the colorectal adenoma-carcinoma sequence.Methods Affymetrix Human U133 PLAS 2.0 GeneChip(covering 18400 transcripts,representing 14500distinct genes)was used to compare different gene expression profiles between normal human colorectal mucosa,colorectal adenoma,Dukes A,B and C-D colorectal cancers.Results A total of 253 different expressed genes among colonrectal adenoma,Dukes A,B and C-D colorectal cancers were identified,among which 34 genes were consistently up-regulated(29 with known function,5 unknown),while 219 othes were consistently down-regulated(196 with known function,23 unknown).Conclusion Onset and further tumor development of colorectal cancer is a complex evolutional course which involves numerous genes.These genes are expressed at the precancerous stage-colorectal adenoma.which indicates the potential of malignant transformation from normal colorectal cell into colorectal cancer is already in existence at the precancerosis,and these genes may participate in the whole process of colorectal carcinogenesis.Therefore,further analysis of obtained genes can help to elucidate the molecular pathogenesis of adenoma-cancer sequence,and is ofguiding significance in the early diagnosis of colorectal cancer and a timely therapeutic intervention.
