国际医药卫生导报
國際醫藥衛生導報
국제의약위생도보
INTERNATIONAL MEDICINE & HEALTH GUIDANCE NEWS
2014年
18期
2799-2803
,共5页
袁晴%刘基铎%刘光平%肖明锋%林汉杰
袁晴%劉基鐸%劉光平%肖明鋒%林漢傑
원청%류기탁%류광평%초명봉%림한걸
原发性胆汁性肝硬化%血清AMA-M2亚型%调节性T细胞%细胞因子
原髮性膽汁性肝硬化%血清AMA-M2亞型%調節性T細胞%細胞因子
원발성담즙성간경화%혈청AMA-M2아형%조절성T세포%세포인자
Primary biliary cirrhosis%AMA-M2%Regulatory T Lymphocytes%Cytokines
目的 研究原发性胆汁性肝硬化(PBC)患者血清抗线粒体抗体亚型M2(autimitochondria antibody typeⅡ,AMA-M2)、调节性T细胞和相关白介素(IL)的表达及相关性,以探讨其诊断价值.方法 分别收集PBC确诊患者组(42例)、疾病对照组(36例)和健康对照组(50例)的血清样本,采用免疫印迹法检测AMA-M2;流式细胞术检测CD4±CD25+调节性T细胞和CD4+、CD8+、CD4+、CD8+水平;IL-2、IL-4、IL-6、IL-8、IL-10采用ELISA定量方法测定.结果 PBC组、健康对照组和疾病对照组AMA-M2阳性率差异有统计学意义(P＜0.05).与AMA-M2阴性组比较,阳性组CD4+、CD4+/CD8+分别增加11.57％和72.79％,CD4+CD25+、CD8+水平分别降低61.50％和35.97％,IL-2、IL-4、IL-6、IL-8、IL-10分别升高189.65％、24.29％、2184.54％、230.33％和1281.37％,且差异均有统计学意义(P＜0.05);但与弱阳性组相比,阳性组血清中只有CD4+、CD8+、IL-4、IL-6、IL-8、IL-10差异有统计学意义(P＜0.05).结论 PBC患者免疫功能异常与IL-2、IL-6、IL-8、IL-10高水平表达相关,血清AMA-M2阳性检测、外周血调节性T细胞比例及细胞因子浓度的检测可为临床诊断PBC提供依据.
目的 研究原髮性膽汁性肝硬化(PBC)患者血清抗線粒體抗體亞型M2(autimitochondria antibody typeⅡ,AMA-M2)、調節性T細胞和相關白介素(IL)的錶達及相關性,以探討其診斷價值.方法 分彆收集PBC確診患者組(42例)、疾病對照組(36例)和健康對照組(50例)的血清樣本,採用免疫印跡法檢測AMA-M2;流式細胞術檢測CD4±CD25+調節性T細胞和CD4+、CD8+、CD4+、CD8+水平;IL-2、IL-4、IL-6、IL-8、IL-10採用ELISA定量方法測定.結果 PBC組、健康對照組和疾病對照組AMA-M2暘性率差異有統計學意義(P＜0.05).與AMA-M2陰性組比較,暘性組CD4+、CD4+/CD8+分彆增加11.57％和72.79％,CD4+CD25+、CD8+水平分彆降低61.50％和35.97％,IL-2、IL-4、IL-6、IL-8、IL-10分彆升高189.65％、24.29％、2184.54％、230.33％和1281.37％,且差異均有統計學意義(P＜0.05);但與弱暘性組相比,暘性組血清中隻有CD4+、CD8+、IL-4、IL-6、IL-8、IL-10差異有統計學意義(P＜0.05).結論 PBC患者免疫功能異常與IL-2、IL-6、IL-8、IL-10高水平錶達相關,血清AMA-M2暘性檢測、外週血調節性T細胞比例及細胞因子濃度的檢測可為臨床診斷PBC提供依據.
목적 연구원발성담즙성간경화(PBC)환자혈청항선립체항체아형M2(autimitochondria antibody typeⅡ,AMA-M2)、조절성T세포화상관백개소(IL)적표체급상관성,이탐토기진단개치.방법 분별수집PBC학진환자조(42례)、질병대조조(36례)화건강대조조(50례)적혈청양본,채용면역인적법검측AMA-M2;류식세포술검측CD4±CD25+조절성T세포화CD4+、CD8+、CD4+、CD8+수평;IL-2、IL-4、IL-6、IL-8、IL-10채용ELISA정량방법측정.결과 PBC조、건강대조조화질병대조조AMA-M2양성솔차이유통계학의의(P＜0.05).여AMA-M2음성조비교,양성조CD4+、CD4+/CD8+분별증가11.57％화72.79％,CD4+CD25+、CD8+수평분별강저61.50％화35.97％,IL-2、IL-4、IL-6、IL-8、IL-10분별승고189.65％、24.29％、2184.54％、230.33％화1281.37％,차차이균유통계학의의(P＜0.05);단여약양성조상비,양성조혈청중지유CD4+、CD8+、IL-4、IL-6、IL-8、IL-10차이유통계학의의(P＜0.05).결론 PBC환자면역공능이상여IL-2、IL-6、IL-8、IL-10고수평표체상관,혈청AMA-M2양성검측、외주혈조절성T세포비례급세포인자농도적검측가위림상진단PBC제공의거.
Objective To study the expressions and correlation of serum anti-mitochondrial M2 antibodies (auti-mitochondria antibody type Ⅱ,AMA-M2),regulatory T cells,and relevant interleukin (IL) in patients with primary biliary cirrhosis (PBC),and to explore the diagnostic value.Methods The serum samples of the PBC diagnosed patients group (n=42),disease control group (n=36),and healthy controls (n=50) were collected respectively.Western Blot Method was used to detect AMA-M2 and the CD4+CD25+ regulatory T cells and the levels of CD4+,CD8+,CD4+/CD8+ were examined by the Flow Cytometry.The ELISA quantitative method was used to determinate IL-4,IL-6,IL-2,IL-8,and IL-10.Results There were statistical differences in the positive rate of AMA-M2 hetween the PBC group,the healthy controls,and the disease control group (P ＜ 0.05).Compared with the AMA-M2 negative group,the levels of CD4+ and CD4+/CD8+ were increased by 11.57％ and 72.79％ respectively,the CD4+CD25+ and CD8+ levels were decreased by 61.50％ and 35.97％ respectively,IL-2,IL-4,IL-6,IL-8,and IL-10 were increased by 189.65％,24.29％,2184.54％,230.33％,and 1281.37％ respectively in the positive group,with statistical differences (P ＜ 0.05).But in comparison with the weak positive group,there were statistical differences in only CD4+/CD8+,IL-4,IL-6,IL-8,and IL-10 between the weak positive and positive groups (P ＜ 0.05).Conclusions The immune dysfunction of the patients with PBC correlates with high expression levels of IL-2,IL-6,IL-8,IL-10.Positive serum AMA-M2 detection,the proportion of regulatory T cell in peripheral blood,and the detection of cytokine concentration can provide references for clinicaly diagnosing PBC.
