CAJ | 학술논문

目的探讨miR-21对绝经后骨质疏松骨髓间充质干细胞(PMOP-hBMMSC)成骨能力影响.方法分离培养正常人(H-hBMMSCs)和绝经后骨质疏松患者(PMOP-hBMMSCs)的骨髓间充质干细胞,并对两组细胞的成骨能力进行比较.转染上调PMOP-hBMMSCs中miR-21表达,观察分析其对成骨能力的影响.Real time RT-PCR和Western Blot比较miR-21在三组细胞间的表达差异,同时检测三组成骨标志性基因Runx2和Osterix的表达.结果 PMOP-hBMMSCs组中的miR-21表达水平、ALP的活性以及钙结节染色面积都高于PMOP组(P＜0.05),与H-hBMMSCs正常对照组差异无统计学意义(P＞0.05);RT-PCR和Western Blot结果显示成骨诱导7d后,转染后高表达Runx2和Osterix mRNA和蛋白水平均高于PMOP组(P＜0.05,P＜0.01),与H-hBMMSCs正常对照组差异无统计学意义(P＞0.05).结论转染miR-21后的PMOP-hBMMSCs成骨能力增强.
목적 탐토miR-21대절경후골질소송골수간충질간세포(PMOP-hBMMSC)성골능력영향.방법 분리배양정상인(H-hBMMSCs)화절경후골질소송환자(PMOP-hBMMSCs)적골수간충질간세포,병대량조세포적성골능력진행비교.전염상조PMOP-hBMMSCs중miR-21표체,관찰분석기대성골능력적영향.Real time RT-PCR화Western Blot비교miR-21재삼조세포간적표체차이,동시검측삼조성골표지성기인Runx2화Osterix적표체.결과 PMOP-hBMMSCs조중적miR-21표체수평、ALP적활성이급개결절염색면적도고우PMOP조(P＜0.05),여H-hBMMSCs정상대조조차이무통계학의의(P＞0.05);RT-PCR화Western Blot결과현시성골유도7d후,전염후고표체Runx2화Osterix mRNA화단백수평균고우PMOP조(P＜0.05,P＜0.01),여H-hBMMSCs정상대조조차이무통계학의의(P＞0.05).결론 전염miR-21후적PMOP-hBMMSCs성골능력증강.
Objective To investigate the osteogenic capability of miR-21 in human bone marrow mesenchymal stem cells in patients with postmenopausal osteoporosis (PMOP-hBMMSCs).Methods The human bone marrow mesenchymal stem cells (hBMMSCs) and PMOP-hBMMSCs were isolated and cultured.The osteogenic capabilities of these two kinds of cells were compared.After over-expression miR-21,the osteogenic potential was analysed in PMOP-hBMMSCs.The expression of Runx2 and Osterix,genes in H-hBMMSCS and PMOP-hBMMSCs were respectively detected by real time RT-PCR and western blot.Results The expression level of miR-21 was higher,ALP was more active,and the calcium nodules staining area was larger in the PMOP-hBMMSCs group than in the PMOP group(P ＜ 0.05).Up-regulation was found in the expressions of Runx2 and Osterix mRNA and protein of Over-expression of miR-21 PMOP-hBMMSCs compared to PMOP(P ＜ 0.05,P ＜ 0.01).Conclusion After the transfection of miR-21,the osteogenic ability of PMOP-hBMMSCs can be enhanced.