CAJ | 학술논문

目的探讨检测缺氧诱导因子-1α(HIF-1α)、环氧化酶-2(COX-2)和C反应蛋白(CRP)用于诊断溃疡性结肠炎的价值.方法 98例溃疡性结肠炎按照病情轻重分为A组(轻度)31例、B组(中度)34例和C组(重度)33例,25例未患溃疡性结肠炎志愿者作为对照组D.采用RT-PCR方法分析各组检查对象结肠组织处HIF-1α、COX-2和C反应蛋白mRNA转录水平.采用ELISA方法检测各组检查对象血清中HIF-1α、COX-2和C反应蛋白水平.结果 HIF-1α mRNA转录水平随着溃疡性结肠炎病情加重而逐渐增加；COX4 mRNA转录水平A组显著高于D组(P＜0.05),B组显著高于A组(P＜0.05),C组与B组差异无统计学意义(P＞0.05)；CRP mRNA转录水平,B组显著高于A组(P＜0.05),C组显著高于B组(P＜0.05),A组与D组差异无统计学意义(P＞0.05).A组、B组和C组血清中HIF-1α、COX-2和C反应蛋白平均水平显著高于对照组D组(P均＜0.05),且随着溃疡性结肠炎病情加重水平逐渐升高.结论病灶细胞中HIF-1α、COX-2和C反应蛋白mRNA转录水平和血清蛋白表达水平与溃疡性结肠炎病情进展呈正相关性,联合检测HIF-1α、COX-2和C反应蛋白有助于判断溃疡性结肠炎病情严重程度.
목적 탐토검측결양유도인자-1α(HIF-1α)、배양화매-2(COX-2)화C반응단백(CRP)용우진단궤양성결장염적개치.방법 98례궤양성결장염안조병정경중분위A조(경도)31례、B조(중도)34례화C조(중도)33례,25례미환궤양성결장염지원자작위대조조D.채용RT-PCR방법분석각조검사대상결장조직처HIF-1α、COX-2화C반응단백mRNA전록수평.채용ELISA방법검측각조검사대상혈청중HIF-1α、COX-2화C반응단백수평.결과 HIF-1α mRNA전록수평수착궤양성결장염병정가중이축점증가；COX4 mRNA전록수평A조현저고우D조(P＜0.05),B조현저고우A조(P＜0.05),C조여B조차이무통계학의의(P＞0.05)；CRP mRNA전록수평,B조현저고우A조(P＜0.05),C조현저고우B조(P＜0.05),A조여D조차이무통계학의의(P＞0.05).A조、B조화C조혈청중HIF-1α、COX-2화C반응단백평균수평현저고우대조조D조(P균＜0.05),차수착궤양성결장염병정가중수평축점승고.결론 병조세포중HIF-1α、COX-2화C반응단백mRNA전록수평화혈청단백표체수평여궤양성결장염병정진전정정상관성,연합검측HIF-1α、COX-2화C반응단백유조우판단궤양성결장염병정엄중정도.
Objective To investigate hypoxia-inducible factor-1α (HIF-1 α),cyclooxygenase-2 (COX-2) and C-reactive protein (CRP) mRNA transcription level of ulcerative colitis pathological tissues and expression levels of HIF-1α,COX-2 and CRP of ulcerative colitis serum.Methods Ninety-eight patients with ulcerative colitis were divided into group A (mild),group B (moderate) and group C (severe)according to the severity,with 25 patients of volunteers as the control group D.RT-PCR was used to analyze mRNA levels of HIF-1α,COX-2 and C-reactive protein in ulcerative colitis pathological tissues.ELISA was used to detect the HIF-1α,COX-2 and C-reactive protein levels of serum.Results HIF-1α mRNA levels gradually increased with the exacerbations of ulcerative colitis.COX-2 mRNA levels of group A were significantly higher than those of Group D (P ＜ 0.05),COX-2 mRNA levels of Group B were significantly higher than of group A (P ＜ 0.05),and COX-2 mRNA levels of group C have no significant difference compared with group B (P ＞ 0.05).CRP mRNA levels of group A have no significant difference compared with group D (P ＞0.05),CRP mRNA levels of group B were significantly higher than those of group A (P ＜0.05),and CRP mRNA of group C were significantly higher than group B (P ＜ 0.05).The average serum protein levels of HIF-1α,COX-2 and C-reactive protein gradually increased with the exacerbations of ulcerative colitis.Conclusion HIF-1α,COX-2 and C-reactive protein mRNA levels of lesion cells and their serum levels were correlated with the progression of ulcerative colitis positively,suggesting that the detection of HIF-1 α,COX-2 and C-reactive protein mRNA levels and expression levels can be used to predict the severity of ulcerative colitis.