中华消化杂志
中華消化雜誌
중화소화잡지
Chinese Journal of Digestion
2012年
11期
750-753
,共4页
结肠炎,溃疡性%HLA-DRB1链%抗体,抗中性粒细胞胞质%人种群
結腸炎,潰瘍性%HLA-DRB1鏈%抗體,抗中性粒細胞胞質%人種群
결장염,궤양성%HLA-DRB1련%항체,항중성립세포포질%인충군
Colitis,ulcerative%HLA-DRB1 chains%Antibodies,antineutrophil cytoplasmic%Ethnic groups
目的 研究人类白细胞抗原(HLA)-DRB1基因多态性与新疆地区维吾尔族及汉族溃疡性结肠炎(UC)患者抗中性粒细胞胞质抗体(ANCA)的关联性.方法 用间接免疫荧光法分别对62例维吾尔族UC患者、58例汉族UC患者、188名维吾尔族健康对照者、184名汉族健康对照者进行血清ANCA检测,采用聚合酶链反应直接测序分型法(PCR-SBT)进行HLA DRB1基因分型,分别在维吾尔族和汉族内比较ANCA阳性者、ANCA阴性者及健康对照者的HLA-DRB1等位基因频率,并按UC临床类型、严重程度、受累范围进行分层分析.应用SPSS 17.0统计软件进行x2检验,当P＜0.05时,计算比值比(OR)和95％可信区间(95％ CI).结果 维吾尔族UC患者ANCA阳性率[53.2％ (33/62)]高于汉族UC患者[34.5％ (20/58)],差异有统计学意义(x2=4.269,P＝0.045).在维吾尔族中,ANCA阳性UC患者HLA-DRB1* 13的基因频率(0.202)显著高于ANCA阴性患者(0.017,x2＝10.092,P＝0.016,OR＝16.000,95％CI:2.892～88.524)和健康对照组(0.075,x2=9.351,P＝0.040,OR=3.407,95％CI:1.666～6.971).ANCA阳性的全结肠炎型UC患者HLA-DRB1* 13基因频率(9/15)高于ANCA阴性的全结肠炎型UC患者(1/14),差异有统计学意义(x2=8.955,P=0.040,OR＝19.500,95％CI:2.787～136.461).在汉族中,HLA-DRB1 各等位基因在ANCA阳性者、ANCA阴性者和健康对照者间差异均无统计学意义(P均＞0.05),分层分析所获结果亦然.结论 在新疆地区维吾尔族UC患者中,HLA-DRB1* 13可能与ANCA有关,还可能与全结肠炎型患者的ANCA有关.在新疆地区汉族患者中则可能无此关联性.
目的 研究人類白細胞抗原(HLA)-DRB1基因多態性與新疆地區維吾爾族及漢族潰瘍性結腸炎(UC)患者抗中性粒細胞胞質抗體(ANCA)的關聯性.方法 用間接免疫熒光法分彆對62例維吾爾族UC患者、58例漢族UC患者、188名維吾爾族健康對照者、184名漢族健康對照者進行血清ANCA檢測,採用聚閤酶鏈反應直接測序分型法(PCR-SBT)進行HLA DRB1基因分型,分彆在維吾爾族和漢族內比較ANCA暘性者、ANCA陰性者及健康對照者的HLA-DRB1等位基因頻率,併按UC臨床類型、嚴重程度、受纍範圍進行分層分析.應用SPSS 17.0統計軟件進行x2檢驗,噹P＜0.05時,計算比值比(OR)和95％可信區間(95％ CI).結果 維吾爾族UC患者ANCA暘性率[53.2％ (33/62)]高于漢族UC患者[34.5％ (20/58)],差異有統計學意義(x2=4.269,P＝0.045).在維吾爾族中,ANCA暘性UC患者HLA-DRB1* 13的基因頻率(0.202)顯著高于ANCA陰性患者(0.017,x2＝10.092,P＝0.016,OR＝16.000,95％CI:2.892～88.524)和健康對照組(0.075,x2=9.351,P＝0.040,OR=3.407,95％CI:1.666～6.971).ANCA暘性的全結腸炎型UC患者HLA-DRB1* 13基因頻率(9/15)高于ANCA陰性的全結腸炎型UC患者(1/14),差異有統計學意義(x2=8.955,P=0.040,OR＝19.500,95％CI:2.787～136.461).在漢族中,HLA-DRB1 各等位基因在ANCA暘性者、ANCA陰性者和健康對照者間差異均無統計學意義(P均＞0.05),分層分析所穫結果亦然.結論 在新疆地區維吾爾族UC患者中,HLA-DRB1* 13可能與ANCA有關,還可能與全結腸炎型患者的ANCA有關.在新疆地區漢族患者中則可能無此關聯性.
목적 연구인류백세포항원(HLA)-DRB1기인다태성여신강지구유오이족급한족궤양성결장염(UC)환자항중성립세포포질항체(ANCA)적관련성.방법 용간접면역형광법분별대62례유오이족UC환자、58례한족UC환자、188명유오이족건강대조자、184명한족건강대조자진행혈청ANCA검측,채용취합매련반응직접측서분형법(PCR-SBT)진행HLA DRB1기인분형,분별재유오이족화한족내비교ANCA양성자、ANCA음성자급건강대조자적HLA-DRB1등위기인빈솔,병안UC림상류형、엄중정도、수루범위진행분층분석.응용SPSS 17.0통계연건진행x2검험,당P＜0.05시,계산비치비(OR)화95％가신구간(95％ CI).결과 유오이족UC환자ANCA양성솔[53.2％ (33/62)]고우한족UC환자[34.5％ (20/58)],차이유통계학의의(x2=4.269,P＝0.045).재유오이족중,ANCA양성UC환자HLA-DRB1* 13적기인빈솔(0.202)현저고우ANCA음성환자(0.017,x2＝10.092,P＝0.016,OR＝16.000,95％CI:2.892～88.524)화건강대조조(0.075,x2=9.351,P＝0.040,OR=3.407,95％CI:1.666～6.971).ANCA양성적전결장염형UC환자HLA-DRB1* 13기인빈솔(9/15)고우ANCA음성적전결장염형UC환자(1/14),차이유통계학의의(x2=8.955,P=0.040,OR＝19.500,95％CI:2.787～136.461).재한족중,HLA-DRB1 각등위기인재ANCA양성자、ANCA음성자화건강대조자간차이균무통계학의의(P균＞0.05),분층분석소획결과역연.결론 재신강지구유오이족UC환자중,HLA-DRB1* 13가능여ANCA유관,환가능여전결장염형환자적ANCA유관.재신강지구한족환자중칙가능무차관련성.
Objective To study the correlation between human leukocyte antigen (HLA)-DRB1 alleles and anti neutrophil cytoplasmatic antibodies (ANCA) in Han and Uygur ulcerative colitis (UC)patients in Xinjiang region.Methods The serum ANCA was determined by indirect immunofluorescence assay in 62 Uygur UC patients,58 Han UC patients,188 Uygur and 184 Han healthy control individuals.HLA-DRB1 typing was performed by polymerase chain reaction-sequence based typing (PCR-SBT).The allele frequency of HLA-DRB1 was compared in ANCA positive and negative Han and Uygur patients as well as healthy controls.Stratified analysis was performed according to UC clinical type,severity and involvement.SPSS 17.0 software was applied for x2 test.Once P＜0.05,the odds ratio (OR) and 95％ confidence intervals (95％CI) was calculated.Results The positive rate of ANCA in Uygur UC patients (53.2％,33/62) was significantly higher than that of Han patients (34.5％,20/58) and the difference was statistically significant (x2=4.269,P =0.045).In Uygur,the gene frequency of HLA-DRB1 * 13 in ANCA positive UC patients (0.202)was significantly higher than that of ANCA negative patients (0.017) (x2 =10.092,P=0.016,OR=16.000,95％CI:2.892 to 88.524) and healthy controls (0.075) (x2=9.351,P＝0.040,OR=3.407,95％CI:1.666 to 6.971).The gene frequency of HLA-DRB1 * 13 in ANCA positive pancolitis type UC patients (9/15) was significantly higher than that of ANCA negative pancolitis type UC patients (1/14) and the difference was statistically significant (x2=8.955,P =0.040,OR =19.500,95％CI:2.787 to 136.461).However,in Han patients,there were no significant differences of HLA-DRB1 alleles frequencies among ANCA positive patients,ANCA negative patients and healthy controls (all P＞0.05),and the results of stratified analysis were same.Conclusions In Uygur UC patients of Xinjiang region,HLA-DRB1 * 13 may correlated with ANCA and with ANCA of pancolitis type UC patients.There is no such correlation in Han patients of Xinjiang region.
