中华消化杂志
中華消化雜誌
중화소화잡지
Chinese Journal of Digestion
2013年
2期
106-110
,共5页
高福利%吕瑛%朱银鑫%曹俊%邹晓平
高福利%呂瑛%硃銀鑫%曹俊%鄒曉平
고복리%려영%주은흠%조준%추효평
DNA(胞嘧啶5-)-甲基转移酶%甲基转移酶类%组蛋白类%组蛋白脱乙酰基酶类%胃肿瘤
DNA(胞嘧啶5-)-甲基轉移酶%甲基轉移酶類%組蛋白類%組蛋白脫乙酰基酶類%胃腫瘤
DNA(포밀정5-)-갑기전이매%갑기전이매류%조단백류%조단백탈을선기매류%위종류
DNA (cytosine 5)-methyhransferase%Methyltransferases%Histones%Histone deacetylases%Stomach neoplasms
目的 研究胃癌细胞和组织中DNA甲基转移酶1(DNMTl)、果蝇zeste基因增强子人类同源基因2(EZH2)和组蛋白去乙酰化酶1(HDACl)的表达及三者的相互作用.方法 实时定量PCR和Western印迹检测MKN28、SGC7901、BGC823、AGS 4株胃癌细胞和正常胃上皮细胞GES-1以及10对新鲜胃癌组织和相应的正常胃组织中DNMT1、EZH2、HDAC1的mRNA和蛋白表达水平；免疫共沉淀检测高分化(MKN28)、中分化(SGC7901)、低分化(BGC823)胃癌细胞和正常胃上皮细胞GES-1及中分化、中-低分化、低分化胃癌组织和正常胃组织中DNMT1、EZH2和HDAC1是否形成复合物.结果 与正常胃上皮细胞和胃组织相比,DNMT1、EZH2和HDAC1在胃癌细胞株和胃癌组织中均高表达,免疫共沉淀检测发现DNMT1、EZH2和HDAC1三者在高、中、低分化胃癌细胞株以及中、中-低、低分化胃癌组织中均形成复合物,但在正常胃上皮细胞和胃组织中不形成复合物.结论 DNMT1、EZH2和HDAC1在胃癌中高表达,并且三者存在相互作用,这可能是胃癌中DNA甲基化与组蛋白修饰存在相关性的重要机制.
目的 研究胃癌細胞和組織中DNA甲基轉移酶1(DNMTl)、果蠅zeste基因增彊子人類同源基因2(EZH2)和組蛋白去乙酰化酶1(HDACl)的錶達及三者的相互作用.方法 實時定量PCR和Western印跡檢測MKN28、SGC7901、BGC823、AGS 4株胃癌細胞和正常胃上皮細胞GES-1以及10對新鮮胃癌組織和相應的正常胃組織中DNMT1、EZH2、HDAC1的mRNA和蛋白錶達水平；免疫共沉澱檢測高分化(MKN28)、中分化(SGC7901)、低分化(BGC823)胃癌細胞和正常胃上皮細胞GES-1及中分化、中-低分化、低分化胃癌組織和正常胃組織中DNMT1、EZH2和HDAC1是否形成複閤物.結果 與正常胃上皮細胞和胃組織相比,DNMT1、EZH2和HDAC1在胃癌細胞株和胃癌組織中均高錶達,免疫共沉澱檢測髮現DNMT1、EZH2和HDAC1三者在高、中、低分化胃癌細胞株以及中、中-低、低分化胃癌組織中均形成複閤物,但在正常胃上皮細胞和胃組織中不形成複閤物.結論 DNMT1、EZH2和HDAC1在胃癌中高錶達,併且三者存在相互作用,這可能是胃癌中DNA甲基化與組蛋白脩飾存在相關性的重要機製.
목적 연구위암세포화조직중DNA갑기전이매1(DNMTl)、과승zeste기인증강자인류동원기인2(EZH2)화조단백거을선화매1(HDACl)적표체급삼자적상호작용.방법 실시정량PCR화Western인적검측MKN28、SGC7901、BGC823、AGS 4주위암세포화정상위상피세포GES-1이급10대신선위암조직화상응적정상위조직중DNMT1、EZH2、HDAC1적mRNA화단백표체수평；면역공침정검측고분화(MKN28)、중분화(SGC7901)、저분화(BGC823)위암세포화정상위상피세포GES-1급중분화、중-저분화、저분화위암조직화정상위조직중DNMT1、EZH2화HDAC1시부형성복합물.결과 여정상위상피세포화위조직상비,DNMT1、EZH2화HDAC1재위암세포주화위암조직중균고표체,면역공침정검측발현DNMT1、EZH2화HDAC1삼자재고、중、저분화위암세포주이급중、중-저、저분화위암조직중균형성복합물,단재정상위상피세포화위조직중불형성복합물.결론 DNMT1、EZH2화HDAC1재위암중고표체,병차삼자존재상호작용,저가능시위암중DNA갑기화여조단백수식존재상관성적중요궤제.
Objective To study the expression and interactions of DNA methyltransferase 1 (DNMT1),enhancer of zeste homolog 2 (EZH2) and histone deacetylase 1 (HDAC1) in gastric cancer cell lines and tissue specimens.Methods The expression of DNMT1,EZH2 and HDAC1 was detected at mRNA and protein level in gastric cancer lines MKN28,SGC7901,BGC823,AGS,normal gastric epithelium cell line GES-1 and 10 pairs of fresh gastric cancer tissues and corresponding normal gastric tissues by real-time polymerase chain reaction and Western blot.Whether DNMT1,EZH2 and HDAC1 forming complex or not was detected by co-immunoprecipitation (Co-IP) in well-differentiated gastric cancer cell line MKN28,medium-differentiated gastric cancer cell line SGC7901,lowdifferentiated gastric cancer cell line BCG823,normal gastric epithelium cell line GES-1,mediumdifferentiated,medium to low-differentiated,low-differentiated gastric cancer tissues and corresponding normal gastric tissues.Results Compared with that of normal gastric epithelium cell and gastric tissue,the expression of DNMT1,EZH2 and HDAC1 in gastric cancer cell lines and gastric tissue was higher.The results of Co-IP indicated that DNMT1,EZH2 and HDAC1 formed complex in the high,medium,and poor differentiated gastric cancer cells and the medium,mediumlow,poor differentiated gastric cancer tissues,but not in normal gastric epithelium cell and tissue.Conclusion DNMT1,EZH2 and HDAC1 highly expressed in gastric cancer and there was interaction effects among them,which might be an important mechanism in the correlation between DNA methylation and histone modifications in gastric cancer.
