中华消化杂志
中華消化雜誌
중화소화잡지
Chinese Journal of Digestion
2013年
3期
166-170
,共5页
孙丹凤%张燕捷%田筱青%房静远
孫丹鳳%張燕捷%田篠青%房靜遠
손단봉%장연첩%전소청%방정원
西罗莫司%蛋白质丝氨酸苏氨酸激酶%信号转导%真核细胞起始因子4E%胃肿瘤
西囉莫司%蛋白質絲氨痠囌氨痠激酶%信號轉導%真覈細胞起始因子4E%胃腫瘤
서라막사%단백질사안산소안산격매%신호전도%진핵세포기시인자4E%위종류
Sirolimus%Protein-serine-threonine kinases%Signal transduction%Eukaryotic initiation factor 4E%Stomach neoplasms
目的 研究哺乳动物雷帕霉素靶蛋白(mTOR)信号通路相关蛋白mTOR和真核起始因子4E结合蛋白1(4E-BP1)在胃癌组织中的活化情况及其临床意义.方法 利用免疫组织化学技术观察38例手术患者胃癌组织中mTOR和4E-BP1的活化情况,采用卡方检验及Kruskal-Wallis检验分析磷酸化mTOR及4E-BP1在癌区、癌旁和正常胃黏膜组织中表达的差异,以及各种临床病理参数间的表达差异.结果 磷酸化mTOR在胃癌组织中的阳性表达率高于匹配的癌旁及正常组织[71.1 ％(27/38)比50.0％(19/38)比44.7 ％(17/38)；x2=11.031,P=0.026],其下游的4E-BP1在胃癌组织中的阳性表达率也高于匹配的癌旁及正常组织[68.4％ (26/38)比57.9％ (22/38)比28.9％(11/38)；x2=13.943,P=0.007].磷酸化mTOR和4E-BP1与肿瘤Lauren' s分型、浸润分期、分化程度、淋巴结转移及患者年龄等无关,但胃癌组织中活化的4E-BP1在不同大小肿瘤间表达差异有统计学意义(H=3.86,P＜0.05).结论 mTOR信号通路在胃癌发生中存在过度激活,其下游蛋白4E-BP1磷酸化程度在不同大小肿瘤中存在差异.
目的 研究哺乳動物雷帕黴素靶蛋白(mTOR)信號通路相關蛋白mTOR和真覈起始因子4E結閤蛋白1(4E-BP1)在胃癌組織中的活化情況及其臨床意義.方法 利用免疫組織化學技術觀察38例手術患者胃癌組織中mTOR和4E-BP1的活化情況,採用卡方檢驗及Kruskal-Wallis檢驗分析燐痠化mTOR及4E-BP1在癌區、癌徬和正常胃黏膜組織中錶達的差異,以及各種臨床病理參數間的錶達差異.結果 燐痠化mTOR在胃癌組織中的暘性錶達率高于匹配的癌徬及正常組織[71.1 ％(27/38)比50.0％(19/38)比44.7 ％(17/38)；x2=11.031,P=0.026],其下遊的4E-BP1在胃癌組織中的暘性錶達率也高于匹配的癌徬及正常組織[68.4％ (26/38)比57.9％ (22/38)比28.9％(11/38)；x2=13.943,P=0.007].燐痠化mTOR和4E-BP1與腫瘤Lauren' s分型、浸潤分期、分化程度、淋巴結轉移及患者年齡等無關,但胃癌組織中活化的4E-BP1在不同大小腫瘤間錶達差異有統計學意義(H=3.86,P＜0.05).結論 mTOR信號通路在胃癌髮生中存在過度激活,其下遊蛋白4E-BP1燐痠化程度在不同大小腫瘤中存在差異.
목적 연구포유동물뢰파매소파단백(mTOR)신호통로상관단백mTOR화진핵기시인자4E결합단백1(4E-BP1)재위암조직중적활화정황급기림상의의.방법 이용면역조직화학기술관찰38례수술환자위암조직중mTOR화4E-BP1적활화정황,채용잡방검험급Kruskal-Wallis검험분석린산화mTOR급4E-BP1재암구、암방화정상위점막조직중표체적차이,이급각충림상병리삼수간적표체차이.결과 린산화mTOR재위암조직중적양성표체솔고우필배적암방급정상조직[71.1 ％(27/38)비50.0％(19/38)비44.7 ％(17/38)；x2=11.031,P=0.026],기하유적4E-BP1재위암조직중적양성표체솔야고우필배적암방급정상조직[68.4％ (26/38)비57.9％ (22/38)비28.9％(11/38)；x2=13.943,P=0.007].린산화mTOR화4E-BP1여종류Lauren' s분형、침윤분기、분화정도、림파결전이급환자년령등무관,단위암조직중활화적4E-BP1재불동대소종류간표체차이유통계학의의(H=3.86,P＜0.05).결론 mTOR신호통로재위암발생중존재과도격활,기하유단백4E-BP1린산화정도재불동대소종류중존재차이.
Objective To investigate the activation and clinical significance of the mammalian target of rapamycin (mTOR) pathway related protein and eukaryotic translation factor 4E-binding protein 1 (4E-BP1) in gastric cancer.Methods The activation of mTOR and 4E-BP1 in gastric cancer tissues of 38 surgical patients were detected by immunohistochemical method.The differences of phosphorylated mTOR and 4E-BP1 expression among cancerous tissues,para-cancerous tissues and normal gastric mucosa tissues and dinicopathological variables were analyzed by Chi square test and Kruskal-Wallis test.Results The positive expression rate of phosphorylated mTOR in the gastric cancerous tissues was significantly higher than that of para-cancerous tissues and normal tissues [71.1％ (27/38),50.0 ％ (19/38) and 44.7 ％ (17/38),x2 =11.031,P =0.026].The positive expression rate of downstream protein 4E-BP1 in the gastric cancer tissues was also significantly higher than that of paracancerous tissues and normal tissues [68.4％(26/38),57.9％(22/38) and 28.9％ (11/38),x2 =13.943,P=0.007].There was no correlation between phosphorylated mTOR and 4E-BP1 expression and tumor Lauren's sub-type,infiltration,differentiation degree,lymph node metastasis and patient's age.There was statistical significant difference between activated 4E-BP1 expression and tumor size in gastric cancer (H=3.86,P＜0.05).Conclsions mTOR pathway was over activated in gastric cancer.There was difference between phosphorylation degree of its downstream protein 4E-BP1 and the tumor size.
