CAJ | 학술논문

目的探讨结直肠癌(CRC)线粒体DNA(mtDNA)拷贝数异常与患者预后的关系.方法选取60份CRC及相应癌旁组织标本,分别提取基因组DNA.对线粒体ND1基因进行荧光定量PCR,计算mtDNA拷贝数,采用t检验进行统计分析.Kaplan-Meier生存分析判断患者的预后.结果 CRC组织的mtDNA平均拷贝数/细胞数为108.60±20.11,而相应的癌旁组织为153.68±25.72,前者显著低于后者(t=10.69,P＜0.01).淋巴结转移阳性者mtDNA低拷贝率高于阴性者(x2=4.022,P＜0.05),但与性别、年龄、病理分型、TNM分期无明显相关性.mtDNA高拷贝组生存率高于低拷贝组,但差异无统计学意义(P＞0.05).结论 CRC的mtDNA拷贝数明显低于癌旁组织,但拷贝数的改变与患者的预后无关.
목적 탐토결직장암(CRC)선립체DNA(mtDNA)고패수이상여환자예후적관계.방법 선취60빈CRC급상응암방조직표본,분별제취기인조DNA.대선립체ND1기인진행형광정량PCR,계산mtDNA고패수,채용t검험진행통계분석.Kaplan-Meier생존분석판단환자적예후.결과 CRC조직적mtDNA평균고패수/세포수위108.60±20.11,이상응적암방조직위153.68±25.72,전자현저저우후자(t=10.69,P＜0.01).림파결전이양성자mtDNA저고패솔고우음성자(x2=4.022,P＜0.05),단여성별、년령、병리분형、TNM분기무명현상관성.mtDNA고고패조생존솔고우저고패조,단차이무통계학의의(P＞0.05).결론 CRC적mtDNA고패수명현저우암방조직,단고패수적개변여환자적예후무관.
Objective To explore the relationship between abnormal copy number of mitochondrial DNA (mtDNA) and prognosis of the patients in colorectal cancer (CRC).Methods A total of 60 cases of CRC and corresponding adjacent noncancerous tissue specimens were selected.Genomic DNA was extracted.The mitochondrial ND1 gene was detected by quantitative real-time polymerase chain reaction and β-actin was set as internal control.And then the copy number of mtDNA was caculated and analyzed by t-test.The prognosis of patients was determined by Kaplan-Meier survival analysis.Results The mean mtDNA copy number of CRC tissue was 108.60±20.11,while that of the corresponding adjacent noncancerous tissues was 153.68±25.72,the former was significantly lower than that of the latter (t=10.69,P＜0.01).The rate of low mtDNA copy number in case with positive lymph nodes metastasis was higher than that in cases with negative lymph nodes metastasis (x2 =4.022,P＜0.05),however there were no obvious correlation with gender,age,pathological type,TNM stage.The survival rate of high mtDNA copy number group was higher than that of low mtDNA copy number group,however there was no statistical significance (P＞ 0.05).Conclusions mtDNA copy number of CRC was significantly lower than that of the adjacent noncancerous tissues.However the change of copy number was not correlated to the prognosis.