中华消化杂志
中華消化雜誌
중화소화잡지
Chinese Journal of Digestion
2013年
3期
191-194
,共4页
宋淑莉%曹海龙%王邦茂%张慧%朐蕊%杨博丽%荆洋%高翔
宋淑莉%曹海龍%王邦茂%張慧%朐蕊%楊博麗%荊洋%高翔
송숙리%조해룡%왕방무%장혜%구예%양박려%형양%고상
小檗碱%腺瘤性息肉%细胞增殖%Wnt蛋白质类%信号转导%疾病模型,动物
小檗堿%腺瘤性息肉%細胞增殖%Wnt蛋白質類%信號轉導%疾病模型,動物
소벽감%선류성식육%세포증식%Wnt단백질류%신호전도%질병모형,동물
Berberine%Adenomatous polyps%Cell proliferation%Wnt proteins%Signal transduction%Disease models%animal
目的 观察小檗碱防治家族性腺瘤性息肉病(FAP)动物模型Apc(Min/+)小鼠息肉生长的疗效并探讨其可能的分子机制.方法 将16只4周龄Apc(Min/+)小鼠均分为对照组(不予处理)和小檗碱组(剂量为150 mg· kg-1·d-1).12周后处死小鼠,记录小鼠肠道息肉数量、大小及分布.免疫组织化学染色法检测小鼠肠道息肉中增殖细胞核抗原(PCNA)、β-链蛋白和细胞周期素D1的表达情况.Western印迹法检测小鼠肠道细胞周期素D1的表达情况.两组间比较行独立样本t检验.结果 小檗碱组小肠及结肠息肉总数较对照组减少66 ％(10.38±1.85比30.50±1.73,t=16.727,P＜0.01).小檗碱组息肉最大径[(1.08±0.65) mm]小于对照组[(1.54±0.62) mm,t=2.114,P=0.041].小檗碱组小鼠肠道息肉中PCNA阳性细胞比例(44.60％±2.88％)较对照组(65.80％±3.27％)减少32％(t=10.875,P＜0.01),β-链蛋白染色异常细胞比例(43.20％±1.63％)较对照组(63.00％±3.08％)减少31％(t=13.956,P＜0.01),细胞周期素D1阳性细胞比例(24.80％±3.11％)较对照组(54.40％±3.78％)减少54％(t=13.510,P＜0.01).小檗碱组肠道细胞周期素D1蛋白表达水平低于对照组.结论 小檗碱可通过干预Wnt通路抑制Apc(Min/+)小鼠肠道息肉的生长,可能成为FAP化学预防的备选药物.
目的 觀察小檗堿防治傢族性腺瘤性息肉病(FAP)動物模型Apc(Min/+)小鼠息肉生長的療效併探討其可能的分子機製.方法 將16隻4週齡Apc(Min/+)小鼠均分為對照組(不予處理)和小檗堿組(劑量為150 mg· kg-1·d-1).12週後處死小鼠,記錄小鼠腸道息肉數量、大小及分佈.免疫組織化學染色法檢測小鼠腸道息肉中增殖細胞覈抗原(PCNA)、β-鏈蛋白和細胞週期素D1的錶達情況.Western印跡法檢測小鼠腸道細胞週期素D1的錶達情況.兩組間比較行獨立樣本t檢驗.結果 小檗堿組小腸及結腸息肉總數較對照組減少66 ％(10.38±1.85比30.50±1.73,t=16.727,P＜0.01).小檗堿組息肉最大徑[(1.08±0.65) mm]小于對照組[(1.54±0.62) mm,t=2.114,P=0.041].小檗堿組小鼠腸道息肉中PCNA暘性細胞比例(44.60％±2.88％)較對照組(65.80％±3.27％)減少32％(t=10.875,P＜0.01),β-鏈蛋白染色異常細胞比例(43.20％±1.63％)較對照組(63.00％±3.08％)減少31％(t=13.956,P＜0.01),細胞週期素D1暘性細胞比例(24.80％±3.11％)較對照組(54.40％±3.78％)減少54％(t=13.510,P＜0.01).小檗堿組腸道細胞週期素D1蛋白錶達水平低于對照組.結論 小檗堿可通過榦預Wnt通路抑製Apc(Min/+)小鼠腸道息肉的生長,可能成為FAP化學預防的備選藥物.
목적 관찰소벽감방치가족성선류성식육병(FAP)동물모형Apc(Min/+)소서식육생장적료효병탐토기가능적분자궤제.방법 장16지4주령Apc(Min/+)소서균분위대조조(불여처리)화소벽감조(제량위150 mg· kg-1·d-1).12주후처사소서,기록소서장도식육수량、대소급분포.면역조직화학염색법검측소서장도식육중증식세포핵항원(PCNA)、β-련단백화세포주기소D1적표체정황.Western인적법검측소서장도세포주기소D1적표체정황.량조간비교행독립양본t검험.결과 소벽감조소장급결장식육총수교대조조감소66 ％(10.38±1.85비30.50±1.73,t=16.727,P＜0.01).소벽감조식육최대경[(1.08±0.65) mm]소우대조조[(1.54±0.62) mm,t=2.114,P=0.041].소벽감조소서장도식육중PCNA양성세포비례(44.60％±2.88％)교대조조(65.80％±3.27％)감소32％(t=10.875,P＜0.01),β-련단백염색이상세포비례(43.20％±1.63％)교대조조(63.00％±3.08％)감소31％(t=13.956,P＜0.01),세포주기소D1양성세포비례(24.80％±3.11％)교대조조(54.40％±3.78％)감소54％(t=13.510,P＜0.01).소벽감조장도세포주기소D1단백표체수평저우대조조.결론 소벽감가통과간예Wnt통로억제Apc(Min/+)소서장도식육적생장,가능성위FAP화학예방적비선약물.
Objective To investigate the prevention and treatment efficacy of polyps growth by berberine in family adenomatous polyposis (FAP) disease Apc (Min/+) mice model mouse and its possible molecular mechanisms.Methods Sixteen four weeks old Apc (Min/+) mice were equally divided into control group (without treatment) and the berberine group (150 mg · kg1 · d1).After 12 weeks,all the mice were sacrificed.The number,size and distribution of mice small intestinal and colonic polyps were recorded.The expressions of proliferating cell nuclear antigen (PCNA),β-catenin and cyclin D1 in mice small intestinal and colonic polyps were detected by the immunohistochemistry method.The expression of cyclin D1 in mice small intestine and colon was examined by Western blot.The comparison between two independent groups was analyzed by t test.Results Compared to the control group,the total number of intestinal and colonic polyps of berberine group decreased 66％％ (10.38± 1.85 vs 30.50± 1.73,t=16.727,P＜0.01).The size of polyps in the berberinegroup [(1.08±0.65) mm] was smaller than that of the control group [(1.54±0.62) mm,t=2.114,P=0.041].The percentage of PCNA positive expression cells in mice small intestinal and colonic polyps of berberine group (44.60％ ± 2.88％) decreased by 32％ compared with the control group (65.80％±3.27％,t =10.875,P＜ 0.01).The percentage of β-catenin staining abnormal cells of berberine group (43.20％±1.63％) decreased by 31％ compared with the control group (63.00％ ±3.08％,t=13.956,P＜0.01).The percentage of cyclin D1 positive cells of berberine group (24.80％±3.11％) decreased by 54％ compared with the control group (54.40％ ±3.78 ％,t=13.510,P＜0.01).The expression of cyclin D1 at protein level in mice small intestine and colon of berberine group was lower than that of control group.Conclusion Berberine can inhibit the growth of small intestinal and colonic polyps in Apc (Min/+) mice through Wnt pathway,and may be a candidate of chemopreventive medicine for FAP.
