中华消化杂志
中華消化雜誌
중화소화잡지
Chinese Journal of Digestion
2013年
11期
761-764
,共4页
叶玉兰%庞智%周春立%郑家驹
葉玉蘭%龐智%週春立%鄭傢駒
협옥란%방지%주춘립%정가구
微RNAs%结肠炎,溃疡性%聚合酶链反应%ROC曲线
微RNAs%結腸炎,潰瘍性%聚閤酶鏈反應%ROC麯線
미RNAs%결장염,궤양성%취합매련반응%ROC곡선
microRNAs%Colitis,ulcerative%Polymerase chain reaction%ROC curve
目的 探讨UC患者外周血血浆中微小核糖核酸(miRNA) 155浓度的诊断价值,及其与UC临床特征的相关性.方法 选取2010年10月至2012年8月确诊的UC患者136例,以同期170名健康体检者作为健康对照.抽取入选者全血,分离提取血浆,按照miRNeasy试剂盒说明用吸附柱提取RNA,用miScript反转录试剂盒将其反转录为cDNA,用miScript SYBR Green PCR试剂盒以cDNA为模板进行miRNA155实时荧光定量PCR.以2-△△Ct法计算miRNA155的相对表达量.组间均数比较行方差分析,miRNA155浓度对UC诊断的价值行ROC曲线分析,miRNA155浓度与UC临床特征的关系行多元线性回归分析.结果 UC患者的血浆miRNA155浓度[(1357.43±326.15) fmol/L]高于健康对照者[(1140.70±312.47) fmol/L],差异有统计学意义(F=35.56,P＜0.01).血浆miRNA155浓度的UC ROC曲线下面积为0.847,95％CI为0.806～0.888,P＜0.01.当血浆miRNA155浓度为1404.51 fmol/L时,其对UC诊断的特异度为94.7％,敏感度为40.4％.血浆miRNA155浓度与UC患者疾病活动性有关(F=12.91,P＜0.05),与疾病严重程度和病变部位无关(P均＞0.05).结论 UC患者血浆中miRNA155高表达,其浓度与UC疾病活动性有关.
目的 探討UC患者外週血血漿中微小覈糖覈痠(miRNA) 155濃度的診斷價值,及其與UC臨床特徵的相關性.方法 選取2010年10月至2012年8月確診的UC患者136例,以同期170名健康體檢者作為健康對照.抽取入選者全血,分離提取血漿,按照miRNeasy試劑盒說明用吸附柱提取RNA,用miScript反轉錄試劑盒將其反轉錄為cDNA,用miScript SYBR Green PCR試劑盒以cDNA為模闆進行miRNA155實時熒光定量PCR.以2-△△Ct法計算miRNA155的相對錶達量.組間均數比較行方差分析,miRNA155濃度對UC診斷的價值行ROC麯線分析,miRNA155濃度與UC臨床特徵的關繫行多元線性迴歸分析.結果 UC患者的血漿miRNA155濃度[(1357.43±326.15) fmol/L]高于健康對照者[(1140.70±312.47) fmol/L],差異有統計學意義(F=35.56,P＜0.01).血漿miRNA155濃度的UC ROC麯線下麵積為0.847,95％CI為0.806～0.888,P＜0.01.噹血漿miRNA155濃度為1404.51 fmol/L時,其對UC診斷的特異度為94.7％,敏感度為40.4％.血漿miRNA155濃度與UC患者疾病活動性有關(F=12.91,P＜0.05),與疾病嚴重程度和病變部位無關(P均＞0.05).結論 UC患者血漿中miRNA155高錶達,其濃度與UC疾病活動性有關.
목적 탐토UC환자외주혈혈장중미소핵당핵산(miRNA) 155농도적진단개치,급기여UC림상특정적상관성.방법 선취2010년10월지2012년8월학진적UC환자136례,이동기170명건강체검자작위건강대조.추취입선자전혈,분리제취혈장,안조miRNeasy시제합설명용흡부주제취RNA,용miScript반전록시제합장기반전록위cDNA,용miScript SYBR Green PCR시제합이cDNA위모판진행miRNA155실시형광정량PCR.이2-△△Ct법계산miRNA155적상대표체량.조간균수비교행방차분석,miRNA155농도대UC진단적개치행ROC곡선분석,miRNA155농도여UC림상특정적관계행다원선성회귀분석.결과 UC환자적혈장miRNA155농도[(1357.43±326.15) fmol/L]고우건강대조자[(1140.70±312.47) fmol/L],차이유통계학의의(F=35.56,P＜0.01).혈장miRNA155농도적UC ROC곡선하면적위0.847,95％CI위0.806～0.888,P＜0.01.당혈장miRNA155농도위1404.51 fmol/L시,기대UC진단적특이도위94.7％,민감도위40.4％.혈장miRNA155농도여UC환자질병활동성유관(F=12.91,P＜0.05),여질병엄중정도화병변부위무관(P균＞0.05).결론 UC환자혈장중miRNA155고표체,기농도여UC질병활동성유관.
Objective To explore the diagnostic value of the concentration of plasma circulation microRNA155 (miRNA155) in ulcerative cilitis (UC) and its correlation with clinical characteristic of UC.Methods From October 2010 to August 2012,a total of 136 patients diagnosed as UC were enrolled,and at same time,170 healthy individuals were set as healthy control.The blood samples of all participants were obtained and plasma was isolated.The adsorption column was used for RNA extraction according to miRNeasy kit instruction.RNA was reverse-transcribed into cDNA with miScript reverse transcription kit.cDNA was a template and miRNA155 real-time quantitative polymerase chain reaction (PCR) was performed with miScript SYBR Green PCR kit.The relative quantity of miRNA155 expression was calculated with 2-△△Ct method.Analysis of variance were performed for comparison between groups.Receiver operating characteristic curve analysis was used for the diagnostic value of miRNA155 concentration in UC.Multiple linear regression analysis was used for the correlation between miRNA155 concentration and clinical characteristics of UC.Results The concentration of plasma circulation miRNA155 of patients with UC ((1357.43±326.15) fmol/L)was higher than that of healthy controls ((1140.70 ± 312.47) fmol/L) and the differences were statistically significant (F=35.56,P＜0.01).The area under receiver operating characteristic curve of the concentration of plasma circulation miRNA155 of patients with UC was 0.847,and the 95 ％CI was 0.806 to 0.888 (P＜0.01).When the concentration of plasma circulation miRNA155 was 1404.51 fmol/L,its specificity in the diagnosis of UC was 94.7％,and sensitivity was 40.4％.There was correlation between the concentration of plasma circulation miRNA155 and the disease activity in patients with UC (F=12.91,P＜0.05).However there was no correlation with the severity and location of the disease (both P＞0.05).Conclusion Plasma circulation miRNA155 highly expressed in patients with UC,and its concentration is correlated with the disease activity.
