CAJ | 학술논문

目的本研究采用犬无菌性心包炎模型来检验N-3多聚不饱和脂肪酸(PUFAs)对心房颤动(房颤)的影响及其对体内炎性因子、心房炎症状态的影响,对PUFAs的抗房颤作用提供新的证据,为房颤的治疗和预防提供新的思路.方法 20只犬随即分为对照组和PUFAs组,分别行开胸制作无菌性心包炎模型.PUFAs组实验开始前4周给予口服PUFAs(2 g/d),术前和术后第2天分别测量心房各项指标(房颤的诱导性和持续性指标、心房有效不应期、房内传导时间)和C-反应蛋白(CRP)、白细胞介素-1β、白细胞介素-6、肿瘤坏死因子-α等水平.结果术前两组间心房各项指标间差异无统计学意义,术后2 d PUFAs组较对照组CRP水平明显降低[(7.6±0.5)mg/dl对(11.7±1.3) mg/dl,P＜0.0001],IL-6水平明显降低[(202.9±23.8) pg/ml对(242.0±19.6) pg/ml,P＜0.001],IL-1β水平明显降低[(149.6±24.8)pg/ml对(233.5±24.8)pg/ml,P=0.0001],TNF-α水平明显降低[(83.3±8.5) pg/ml对(112.4±8.2)pg/ml,P＜0.0001],房颤可诱导性(11.0±7.4对28.0±10.3,P＜0.001)和持续性明显降低[房颤时程中位数:1105 s(655.8～ 1406.5 s)对2516.5 s(1187～3361 s),P＜0.05],并且较对照组心房有效不应期(AERP)明显延长[(133.4±4.1)ms对(129.8±4.3)ms,P＜0.05],房内传导时间(CT)明显缩短[(46.6±4.4)ms对(51.9±4.8)ms,P＜0.05].结论 PUFAs可以明显降低术后房颤的发生危险,其对炎症反应的抑制作用可能是其机制所在之一.
목적 본연구채용견무균성심포염모형래검험N-3다취불포화지방산(PUFAs)대심방전동(방전)적영향급기대체내염성인자、심방염증상태적영향,대PUFAs적항방전작용제공신적증거,위방전적치료화예방제공신적사로.방법 20지견수즉분위대조조화PUFAs조,분별행개흉제작무균성심포염모형.PUFAs조실험개시전4주급여구복PUFAs(2 g/d),술전화술후제2천분별측량심방각항지표(방전적유도성화지속성지표、심방유효불응기、방내전도시간)화C-반응단백(CRP)、백세포개소-1β、백세포개소-6、종류배사인자-α등수평.결과 술전량조간심방각항지표간차이무통계학의의,술후2 d PUFAs조교대조조CRP수평명현강저[(7.6±0.5)mg/dl대(11.7±1.3) mg/dl,P＜0.0001],IL-6수평명현강저[(202.9±23.8) pg/ml대(242.0±19.6) pg/ml,P＜0.001],IL-1β수평명현강저[(149.6±24.8)pg/ml대(233.5±24.8)pg/ml,P=0.0001],TNF-α수평명현강저[(83.3±8.5) pg/ml대(112.4±8.2)pg/ml,P＜0.0001],방전가유도성(11.0±7.4대28.0±10.3,P＜0.001)화지속성명현강저[방전시정중위수:1105 s(655.8～ 1406.5 s)대2516.5 s(1187～3361 s),P＜0.05],병차교대조조심방유효불응기(AERP)명현연장[(133.4±4.1)ms대(129.8±4.3)ms,P＜0.05],방내전도시간(CT)명현축단[(46.6±4.4)ms대(51.9±4.8)ms,P＜0.05].결론 PUFAs가이명현강저술후방전적발생위험,기대염증반응적억제작용가능시기궤제소재지일.
Objective It has been recently reported that atrial fibrillation (AF)is associated with tissue inflammation and inflammatory cytokines,and N-3 polyunsaturated fatty acids (PUFAs)have been reported anti-inflammatory effects in some epidemiological studies.However,the anti-inflammatory effect of PUFAs on AF is unclear.The purpose of the present study was to evaluate the anti-inflammatory effect of PUFAs on AF in a canine sterile pericarditis model.Methods Twenty dogs were randomly assigned to two groups:a control group (10 dogs)and a PUFAs treatment group (10 dogs),in which sterile pericarditis was created by open-chest operation.PUFAs was administered orally(2 g/d)beginning 4 week before the operation until the end of the study.Before and 2 days after the operation,levels of CRP,IL-6,IL-1β,TNF-α,the inducibility and maintenance of AF,the atrial effective refractory period(AERP),and intra-atrial conduction time(CT) were measured.Results Before the operation,there were no significant differences in any of the parameters between the two groups.On the second postoperative day,the PUFAs group had a lower CRP level [(7.6±0.5)mg/dl vs.(11.7± 1.3)] mg/dl,P＜0.0001,a lower IL-6 level[(202.9±23.8) pg/ml vs.(242.0±19.6) pg/ml,P＜0.0001],a lower IL-1β level[(149.6±24.8) pg/ml vs.(233.5±24.8)pg/ml,P=0.0001],a lower TNF-α level[(83.3±8.50 pg/ml vs.(112.4±8.2) pg/ml,P＜0.0001],a less AF inducibility(percentage of burst attempts leading to AF episodes:(11.0±7.4 vs.28.0± 10.3,P＜0.001) and maintenance [median AF duration:1105 s (655.8 ～ 1406.5 s) vs.2516.5 s(1187～3361 s),P＜0.05],a longer AERP[(133.4±4.1) ms vs.(129.8±4.3) ms,P＜0.05],and a shorter intra-atrial conduction time [(46.6 ± 4.4) ms vs.(51.9 ± 4.8) ms,P ＜ 0.05] than that in the control group.Conclusion Dietary N-3 PUFAs supplementation attenuates the inducibility and maintenance of AF in an animal model of sterile pericarditis.Depression of proinflammatory cytokines by N-3 PUFAs probably contributes to the antiarrhythmic effects.