中华显微外科杂志
中華顯微外科雜誌
중화현미외과잡지
Chinese Journal of Microsurgery
2013年
2期
144-148
,共5页
许银峰%胡军%肖良宝%江丽%易建华%牛晓锋
許銀峰%鬍軍%肖良寶%江麗%易建華%牛曉鋒
허은봉%호군%초량보%강려%역건화%우효봉
肌腱%化学萃取%去细胞%磷酸三丁酯%曲拉通-100
肌腱%化學萃取%去細胞%燐痠三丁酯%麯拉通-100
기건%화학췌취%거세포%린산삼정지%곡랍통-100
Tendon%Chemical extraction%Acellular%TnBP%Triton X-100
目的 探索化学萃取法制备去细胞人体肌腱支架材料的实验方法. 方法 用1.0％磷酸三丁酯[tri(n-butyl)Phosphate,TnBP]分别联合0％、0.25％、0.50％及1.00％曲拉通-100(Triton X-100)对人体肌腱进行去细胞处理,通过组织学、扫描电镜观察、生物力学和羟脯氨酸含量测定,并与新鲜人体肌腱对比,评价去细胞人体肌腱支架材料的特性. 结果 肌腱去细胞处理后色泽光亮,腱膜完整,韧性好；1.00％ TnBP加0％ Triton X-100组仍有少量细胞存留,其余3个处理组去细胞彻底,1.00％TnBP加0.25％ Triton X-100组胶原纤维完整、走形规则.1.00％ TnBP加0.50％ Triton X-100与1.00％TnBP加1.00％ Triton X-100组胶原纤维完整,但间隙略增宽,最大载荷分别为(385.220±80.316)N、(398.220±127.195)N,抗张强度分别为(46.690±16.295) Mpa、(46.20 ±5.517) Mpa,羟脯氨酸含量分别为(0.2826630±0.0110109) μg/mg、(0.2793550±0.0102129)μ/mg,分别和新鲜对照组[最大载荷(533.280±135.774)N、抗张强度(65.560±14.401) Mpa、羟脯氨酸含量(0.2928820±0.0100988)μ/mg]相比,力学性能降低、羟脯氨酸含量减少,差异有统计学意义(P＜0.05). 结论 1.00％ TnBP加0.25％ Triton X-100处理是较理想的制备去细胞人体肌腱的方法.
目的 探索化學萃取法製備去細胞人體肌腱支架材料的實驗方法. 方法 用1.0％燐痠三丁酯[tri(n-butyl)Phosphate,TnBP]分彆聯閤0％、0.25％、0.50％及1.00％麯拉通-100(Triton X-100)對人體肌腱進行去細胞處理,通過組織學、掃描電鏡觀察、生物力學和羥脯氨痠含量測定,併與新鮮人體肌腱對比,評價去細胞人體肌腱支架材料的特性. 結果 肌腱去細胞處理後色澤光亮,腱膜完整,韌性好；1.00％ TnBP加0％ Triton X-100組仍有少量細胞存留,其餘3箇處理組去細胞徹底,1.00％TnBP加0.25％ Triton X-100組膠原纖維完整、走形規則.1.00％ TnBP加0.50％ Triton X-100與1.00％TnBP加1.00％ Triton X-100組膠原纖維完整,但間隙略增寬,最大載荷分彆為(385.220±80.316)N、(398.220±127.195)N,抗張彊度分彆為(46.690±16.295) Mpa、(46.20 ±5.517) Mpa,羥脯氨痠含量分彆為(0.2826630±0.0110109) μg/mg、(0.2793550±0.0102129)μ/mg,分彆和新鮮對照組[最大載荷(533.280±135.774)N、抗張彊度(65.560±14.401) Mpa、羥脯氨痠含量(0.2928820±0.0100988)μ/mg]相比,力學性能降低、羥脯氨痠含量減少,差異有統計學意義(P＜0.05). 結論 1.00％ TnBP加0.25％ Triton X-100處理是較理想的製備去細胞人體肌腱的方法.
목적 탐색화학췌취법제비거세포인체기건지가재료적실험방법. 방법 용1.0％린산삼정지[tri(n-butyl)Phosphate,TnBP]분별연합0％、0.25％、0.50％급1.00％곡랍통-100(Triton X-100)대인체기건진행거세포처리,통과조직학、소묘전경관찰、생물역학화간포안산함량측정,병여신선인체기건대비,평개거세포인체기건지가재료적특성. 결과 기건거세포처리후색택광량,건막완정,인성호；1.00％ TnBP가0％ Triton X-100조잉유소량세포존류,기여3개처리조거세포철저,1.00％TnBP가0.25％ Triton X-100조효원섬유완정、주형규칙.1.00％ TnBP가0.50％ Triton X-100여1.00％TnBP가1.00％ Triton X-100조효원섬유완정,단간극략증관,최대재하분별위(385.220±80.316)N、(398.220±127.195)N,항장강도분별위(46.690±16.295) Mpa、(46.20 ±5.517) Mpa,간포안산함량분별위(0.2826630±0.0110109) μg/mg、(0.2793550±0.0102129)μ/mg,분별화신선대조조[최대재하(533.280±135.774)N、항장강도(65.560±14.401) Mpa、간포안산함량(0.2928820±0.0100988)μ/mg]상비,역학성능강저、간포안산함량감소,차이유통계학의의(P＜0.05). 결론 1.00％ TnBP가0.25％ Triton X-100처리시교이상적제비거세포인체기건적방법.
Objective To explore a method of preparing the acellular tendons of human with chemical approaches.Methods From April 2011 to June 2012,several treatments were performed on human tendons using 1.00％ tri(n-butyl) Phosphate (TnBP) combinded with Triton X-100 at the concentration of 0,0.25％,0.50％,1.00％ respectively.Specimens were examined using histological observation,scanning electron microscopy,biomechanical testing and hydroxyproline quantitation.The results were then compared with fresh tendons of control group,so as to value the characteristics of decellularized human tendon scaffold.Results Acellular human tendons had glossy surface,intact aponeurotic membrane and satisfactory flexibility.A small number of disrupted cells remained in the 1.00％ TnBP + 0％ Triton X-100 treated tissue,while other three experimental groups successfully eliminate all cells.Intact and regular collagen architecture was retained in 1.00％ TnBP +0.25％ Triton X-100 treated tissue.1.00％ TnBP + 0.50％ Triton X-100 and 1.00％ TnBP + 1.00％ Triton X-100 treated tissue were nearly identical to 1.00％ TnBP +0.25％ Triton X-100 treated tissue,but the interval of collagen was slightly wider than the control group,the maximum load (385.22 ± 80.32N,398.22 ± 127.20N),ultimate tensile strength(46.69 ± 16.30Mpa,46.20 ±5.52Mpa) and hydroxyproline content(0.282663 ± 0.0110109 μg/mg,0.279355 ± 0.0102129 μg/mg) were statistically lower (P ＜ 0.05) compared with those of the control group,maximum load(533.28 ± 135.77N),ultimate tensile strength (65.56 ± 14.40Mpa) and hydroxyproline content (0.292882 ± 0.0100988 μg/mg) respectively.Conclusion The decellularization treatment with 1.00％ TnBP + 0.25％ Triton X-100 could be optimized for preparing acellular human tendons.
