中华显微外科杂志
中華顯微外科雜誌
중화현미외과잡지
Chinese Journal of Microsurgery
2013年
4期
352-355
,共4页
董苑%杨瑞年%王福科%刘流
董苑%楊瑞年%王福科%劉流
동원%양서년%왕복과%류류
骨髓间充质干细胞%血管内皮细胞%联合培养%骨形态发生蛋白2
骨髓間充質榦細胞%血管內皮細胞%聯閤培養%骨形態髮生蛋白2
골수간충질간세포%혈관내피세포%연합배양%골형태발생단백2
Bone marrow stromal cellsc (BMSCs)%Vascular endothelial cells (VECs)%Co-culture system%Bone morphogenetic protein 2 (BMP2)
目的 研究BMP2在hBMSCs和hUVECs体外联合培养系统中对细胞增殖和成骨分化的调控作用. 方法 选用第三代hUVECs、hBMSCs细胞以及经BMP2特异性静默干扰处理的hUVECs细胞建立联合培养体系.观察不同培养组单独hBMSCs组、hUVECs与hBMSCs细胞联合培养组以及经BMP2蛋白静默干扰处理hUVECs与hBMSCs细胞联合培养干扰组中BMSCs数量,运用酶活性法和放射免疫法分别对碱性磷酸酶及骨钙素的表达进行检测. 结果 在BMP2静默的hUVECs联合hBMSCs培养干扰组观察到随BMP2蛋白分泌减少,hBMSCs的细胞数目下降,但仍高于单独hBMSCs培养组,差异有统计学意义(P＜0.05).联合培养体系中运用RNA干扰技术静默血管内皮细胞对BMP2的表达后,联合培养干扰组内hBMSCs对碱性磷酸酶、骨钙素在同一时间点表达低于联合培养组,但仍然高于单独间充质干细胞培养组,差异有统计学意义(P＜0.05). 结论 联合培养体系中血管内皮细胞分泌的BMP2在BMSCs增殖和成骨分化诱导方面起着重要作用.
目的 研究BMP2在hBMSCs和hUVECs體外聯閤培養繫統中對細胞增殖和成骨分化的調控作用. 方法 選用第三代hUVECs、hBMSCs細胞以及經BMP2特異性靜默榦擾處理的hUVECs細胞建立聯閤培養體繫.觀察不同培養組單獨hBMSCs組、hUVECs與hBMSCs細胞聯閤培養組以及經BMP2蛋白靜默榦擾處理hUVECs與hBMSCs細胞聯閤培養榦擾組中BMSCs數量,運用酶活性法和放射免疫法分彆對堿性燐痠酶及骨鈣素的錶達進行檢測. 結果 在BMP2靜默的hUVECs聯閤hBMSCs培養榦擾組觀察到隨BMP2蛋白分泌減少,hBMSCs的細胞數目下降,但仍高于單獨hBMSCs培養組,差異有統計學意義(P＜0.05).聯閤培養體繫中運用RNA榦擾技術靜默血管內皮細胞對BMP2的錶達後,聯閤培養榦擾組內hBMSCs對堿性燐痠酶、骨鈣素在同一時間點錶達低于聯閤培養組,但仍然高于單獨間充質榦細胞培養組,差異有統計學意義(P＜0.05). 結論 聯閤培養體繫中血管內皮細胞分泌的BMP2在BMSCs增殖和成骨分化誘導方麵起著重要作用.
목적 연구BMP2재hBMSCs화hUVECs체외연합배양계통중대세포증식화성골분화적조공작용. 방법 선용제삼대hUVECs、hBMSCs세포이급경BMP2특이성정묵간우처리적hUVECs세포건립연합배양체계.관찰불동배양조단독hBMSCs조、hUVECs여hBMSCs세포연합배양조이급경BMP2단백정묵간우처리hUVECs여hBMSCs세포연합배양간우조중BMSCs수량,운용매활성법화방사면역법분별대감성린산매급골개소적표체진행검측. 결과 재BMP2정묵적hUVECs연합hBMSCs배양간우조관찰도수BMP2단백분비감소,hBMSCs적세포수목하강,단잉고우단독hBMSCs배양조,차이유통계학의의(P＜0.05).연합배양체계중운용RNA간우기술정묵혈관내피세포대BMP2적표체후,연합배양간우조내hBMSCs대감성린산매、골개소재동일시간점표체저우연합배양조,단잉연고우단독간충질간세포배양조,차이유통계학의의(P＜0.05). 결론 연합배양체계중혈관내피세포분비적BMP2재BMSCs증식화성골분화유도방면기착중요작용.
Objective To investigate the effects of BMP2 in the co-culture system of hBMSCs and hUVECs.Methods Based on the co-culture system of cells,a simple culture group (hBMSCs group),a combined culture group (BMSCs + hUVECs group) and a BMP2 silenced group (BMSCs + BMP2 silenced hUVECs) were established.On days 4,6,8,10,cells were counted for drawing cell growth curves.In addition,ALP and OC expression in BMSCs was detected in each group.Results At each time point,cell number in the BMSCs + hUVECs group were significantly greater than in the BMSCs group,while the BMP2 silenced group were in the median.ALP and OC expression in the BMSCs + hUVECs group were significantly greater than in the BMSCs group,while the BMP2 silenced group were in the median.Conclusion BMP2 plays an important role in the co-culture system,which can promote osteogenic differentiation and the growth of BMSCs.
