CAJ | 학술논문

目的通过体外实验探讨新型钌(Ⅱ)多吡啶配合物△-[Ru(phen)2MCMIP]2+(△-1)对人骨肉瘤细胞MG-63株增殖及凋亡作用. 方法 CCK-8法检测经0.00、12.50、25.00、50.00、100.00、150.00 μmol/L△-1作用24、48、72 h后,MG-63细胞的增殖情况；流式细胞术检测经0.00、25.00、50.00、100.00 μmol/L△-1作用24h后,MG-63细胞的凋亡及周期变化. 结果 △-1可明显抑制人骨肉瘤MG-63细胞增殖,并呈明显剂量和时间依赖性；24、48、72 h IC5o分别为57.80 μmol/L、45.27 μmol/L、32.51 μmol/L；流式细胞术检测得不同浓度△-1作用24h后,细胞早期凋亡比例从(1.06±0.12)％上升至(37.10±1.25)％,细胞周期被阻滞在Go/G1期. 结论 △-1能抑制MG-63细胞增殖,诱导细胞凋亡,并将细胞周期阻滞在Go/G1期.
목적 통과체외실험탐토신형조(Ⅱ)다필정배합물△-[Ru(phen)2MCMIP]2+(△-1)대인골육류세포MG-63주증식급조망작용. 방법 CCK-8법검측경0.00、12.50、25.00、50.00、100.00、150.00 μmol/L△-1작용24、48、72 h후,MG-63세포적증식정황；류식세포술검측경0.00、25.00、50.00、100.00 μmol/L△-1작용24h후,MG-63세포적조망급주기변화. 결과 △-1가명현억제인골육류MG-63세포증식,병정명현제량화시간의뢰성；24、48、72 h IC5o분별위57.80 μmol/L、45.27 μmol/L、32.51 μmol/L；류식세포술검측득불동농도△-1작용24h후,세포조기조망비례종(1.06±0.12)％상승지(37.10±1.25)％,세포주기피조체재Go/G1기. 결론 △-1능억제MG-63세포증식,유도세포조망,병장세포주기조체재Go/G1기.
Objective To investigate the effects of a novel ruthenium(Ⅱ) polypyridyl complex △-[Ru (phen)2MCMIP]2 + (△-1) on proliferation and apoptosis of human osteosarcoma cell line MG-63 in vitro.Methods Cell counting of kit-8 (CCK-8) assay was used to detect the proliferation of MG-63 cells after 24,48,72h treatment with △-1 under the concentrations of 0.00,12.50,25.00,50.00,100.00,150.00 μmol/L;Changes of apoptosis and cell cycle in MG-63 cells after 24 h treatment of 0.00,25.00,50.00,100.00 μmol/L A-1 were determined and analyzed by flow cytometry (FCM).Results Ruthenium (Ⅱ) polypyridyl complex A-1 could significantly inhibit the growth of MG-63 in a dose and time dependent manner; the IC50 of 24 h,48 h,72 h was 57.80μmol/L,45.27μmol/L,32.51μmol/L respectively; flow cytometry detection showed that A-1 induced 37.10％ of apoptosis,while only 1.06％ in control group,and arrested cell cycle at G0/G1 phase.Conclusion Ruthenium(Ⅱ) polypyridyl complex A-1 is able to inhibit proliferation and induce apoptosis in MG-63 cells and arrest cell cycle at G0/G1 phase.