中华显微外科杂志
中華顯微外科雜誌
중화현미외과잡지
Chinese Journal of Microsurgery
2014年
1期
44-47
,共4页
臂丛%神经损伤%手术时机%大鼠
臂叢%神經損傷%手術時機%大鼠
비총%신경손상%수술시궤%대서
Brachial plexus%Nerve injury%Operative timing%Rat
目的 研究不同时间神经移植术修复幼年大鼠臂丛损伤后对远端靶组织的影响. 方法 2009年1月至12月,将出生18 d SD大鼠48只随机等分为8组:第5颈神经(颈5)切断组和立即修复组及3、6、9、12、15、18 d修复组.颈5切断组将右侧颈5神经根切除0.3 cm;立即修复组将颈5神经根切除后取腓肠神经行移植修复.其余各组分别在颈5切断后间隔相应时间修复.术后6周,进行肌肉功能检测,包括肌肉湿重、肌肉截面积、有髓神经纤维计数、CMAP的潜伏期及波幅,比较各组靶组织功能的差异. 结果 幼年大鼠颈5切断后,各组再生有髓神经纤维计数、肌肉湿重、肌肉截面积、CMAP波幅、CMAP潜伏期总体差异有统计学意义(P＜0.05).立即修复后运动功能恢复效果与3、6d修复组相同,神经根切断15 d以后修复靶组织功能恢复差(P＜0.05). 结论 幼年大鼠神经根损伤后,在0～6d(相当于人类0～4个月)内修复可以有效地恢复远端靶组织(肌肉)的功能.这提示临床上对有手术指征的产瘫应争取在出生后4个月内手术.
目的 研究不同時間神經移植術脩複幼年大鼠臂叢損傷後對遠耑靶組織的影響. 方法 2009年1月至12月,將齣生18 d SD大鼠48隻隨機等分為8組:第5頸神經(頸5)切斷組和立即脩複組及3、6、9、12、15、18 d脩複組.頸5切斷組將右側頸5神經根切除0.3 cm;立即脩複組將頸5神經根切除後取腓腸神經行移植脩複.其餘各組分彆在頸5切斷後間隔相應時間脩複.術後6週,進行肌肉功能檢測,包括肌肉濕重、肌肉截麵積、有髓神經纖維計數、CMAP的潛伏期及波幅,比較各組靶組織功能的差異. 結果 幼年大鼠頸5切斷後,各組再生有髓神經纖維計數、肌肉濕重、肌肉截麵積、CMAP波幅、CMAP潛伏期總體差異有統計學意義(P＜0.05).立即脩複後運動功能恢複效果與3、6d脩複組相同,神經根切斷15 d以後脩複靶組織功能恢複差(P＜0.05). 結論 幼年大鼠神經根損傷後,在0～6d(相噹于人類0～4箇月)內脩複可以有效地恢複遠耑靶組織(肌肉)的功能.這提示臨床上對有手術指徵的產癱應爭取在齣生後4箇月內手術.
목적 연구불동시간신경이식술수복유년대서비총손상후대원단파조직적영향. 방법 2009년1월지12월,장출생18 d SD대서48지수궤등분위8조:제5경신경(경5)절단조화립즉수복조급3、6、9、12、15、18 d수복조.경5절단조장우측경5신경근절제0.3 cm;립즉수복조장경5신경근절제후취비장신경행이식수복.기여각조분별재경5절단후간격상응시간수복.술후6주,진행기육공능검측,포괄기육습중、기육절면적、유수신경섬유계수、CMAP적잠복기급파폭,비교각조파조직공능적차이. 결과 유년대서경5절단후,각조재생유수신경섬유계수、기육습중、기육절면적、CMAP파폭、CMAP잠복기총체차이유통계학의의(P＜0.05).립즉수복후운동공능회복효과여3、6d수복조상동,신경근절단15 d이후수복파조직공능회복차(P＜0.05). 결론 유년대서신경근손상후,재0～6d(상당우인류0～4개월)내수복가이유효지회복원단파조직(기육)적공능.저제시림상상대유수술지정적산탄응쟁취재출생후4개월내수술.
Objective To disclose the relationship of the target muscle function and different time interval after nerve grafting reconstructed C5 root resection in young rats.Methods Model of C5 resection was set up in 48 18-day-old SD rats.The rats were randomly divided into C5 resection group,immediate repairing group,3 days delayed repairing group,and 6,9,12,15,18 days delayed repairing groups.Each group experienced nerve grafting bridged the C5 nerve root defection at its time interval.At 6 weeks postoperatively,electrophysiological and histochemical experiment were performed.Results There was no statistical difference among the data of CMAP amplitude and latency and weight of target muscles and number of distal myelinated fiber of immediate repairing group and those of 3,6 days delayed repair group at 6 weeks postoperatively,but compared with C5 resection group,the dates was statistically higher.There was no statistical difference between the data of C5 resection group and that of 15,18 days delayed repairing group.Conclusion Nerve reconstruction for C5 root injury in young rats within 0-6 days (equal to 0-4 months in human beings) has a satisfactory protective effect on target muscles.It suggests that the OBPP children who have the operation indication should undergo surgical management in 4 months after their birth.
