CAJ | 학술논문

目的通过在体外观察乳鼠心室细胞在不同处理的室间隔缺损封堵器材料上的生长情况,探讨室间隔缺损介入封堵治疗后影响封堵器表面愈合填补的因素.方法根据室间隔缺损封堵器材料是否有胶原蛋白涂层,将实验分为涂层组和非涂层组,各组又分为3个亚组,分别给予表皮生长因子(EGF组)、5-溴脱氧尿苷(BrdU组)处理及不额外处理(对照组).乳鼠心室肌组织分别与不同处理的封堵器材料(1 cm×2 cm)进行培养,观察组织块开始搏动时间,细胞开始爬行时间,封堵器材料表面生长的细胞种类、含晕及胰岛素样生长因子的含量.结果 (1)涂层组和非涂层组间组织块开始搏动时间差异无统计学意义.亚组中BrdU组开始搏动时间早于EGF组及对照组(P<0.05).(2)涂层组细胞开始爬行时间早于非涂层组(P<0.05).亚组中EGF组开始爬行时间最早(P<0.05).(3)在封堵器材料上生长的细胞包括内皮细胞、成纤维细胞和心肌细胞.内皮细胞含量在EGF组最高,成纤维细胞含量在BrdU组最高,心肌细胞含昔BrdU组与对照组均高于EGF组(P<0.05).(4)各组胰岛素样生长因子的阳性率均在培养的第7天达到最高值.结论胶原蛋白涂层促进细胞爬行,EGF促进内皮细胞的生长,BrdU可在不影响心肌细胞生长的状态下促进成纤维细胞的生长.
목적 통과재체외관찰유서심실세포재불동처리적실간격결손봉도기재료상적생장정황,탐토실간격결손개입봉도치료후영향봉도기표면유합전보적인소.방법 근거실간격결손봉도기재료시부유효원단백도층,장실험분위도층조화비도층조,각조우분위3개아조,분별급여표피생장인자(EGF조)、5-추탈양뇨감(BrdU조)처리급불액외처리(대조조).유서심실기조직분별여불동처리적봉도기재료(1 cm×2 cm)진행배양,관찰조직괴개시박동시간,세포개시파행시간,봉도기재료표면생장적세포충류、함훈급이도소양생장인자적함량.결과 (1)도층조화비도층조간조직괴개시박동시간차이무통계학의의.아조중BrdU조개시박동시간조우EGF조급대조조(P<0.05).(2)도층조세포개시파행시간조우비도층조(P<0.05).아조중EGF조개시파행시간최조(P<0.05).(3)재봉도기재료상생장적세포포괄내피세포、성섬유세포화심기세포.내피세포함량재EGF조최고,성섬유세포함량재BrdU조최고,심기세포함석BrdU조여대조조균고우EGF조(P<0.05).(4)각조이도소양생장인자적양성솔균재배양적제7천체도최고치.결론 효원단백도층촉진세포파행,EGF촉진내피세포적생장,BrdU가재불영향심기세포생장적상태하촉진성섬유세포적생장.
Objective To observe the effects of collagen-coating, epidermal growth factor (EGF), Bromodeoxyuridine (BrdU) on growth and function of neonatal ventricular cardiomyocytes in transcatheter closure device patches in vitro. Methods Neonatal ventricular cardiomyocytes were cultured with transcatheter closure device patches (1 cm × 2 cm) coated with or without collagen and treated with 10% FBS (control), EGF (20 ng/ml), BrdU (0.1 mmol/L), respectively. In vitro ventricular cardiomyocytes growth and function as well as IGF-I content were determined. Results (1) The beginning time of ventricular cardiomyocytes beating on patches was similar in collagen-coated and uncoated patches treated with PBS, EGF or BrdU, respectively (P > 0.05). The cell beating time was significantly earlier in Brdu group than in PBS and EGF groups (all P < 0.05). (2) Time of cultured cell covering on patches was significantly earlier in coated patches than those uncoated patches in PBS, EGF and BrdU treated groups (all P < 0.05). The ventricular cardiomyocytes covering time on patches was significantly earlier in EGF group than that in PBS and BrdU groups (all P < 0.05). (3) Ventricular cardiomyocytes types survived on patches included endothelial cells, fibroblasts and myocytes. The highest content of endothelial cells was evidenced in EGF group and the highest content of fibroblasts was found in Brdu group. Myocytes content was similar between PBS and BrdU groups (P > 0.05) and significantly higher than that in EGF group (all P < 0.05). (4) IGF-I peaked at the seventh culture day in all groups (all P < 0.01). Conclusions Ventricular cardiomyocytes covering on patches could be enhanced by collagen coating. EGF could promote endothelial cells growth while Brdu could stimulate fibroblasts growth on patches.