CAJ | 학술논문

目的探讨前列腺六次跨膜蛋白-2(STAMP2)基因多态性与维吾尔族人群中心性肥胖的相关性.方法采取以流行病学调查为基础的病例对照研究方法,选择2332例维吾尔族人为研究对象,其中1455例中心性肥胖者作为肥胖组,其余877例非肥胖者作为对照组.测序筛查STAMP2基因变异位点后,以TaqMan-PCR技术对代表性变异位点进行基因分型,分析STAMP2基因多态性与维吾尔族中心性肥胖的相关性.结果测序发现20个变异,包括14个新发现的变异位点.肥胖组STAMP2基因rs1981529的AA基因型频率高于对照组(67.6％比62.8％,P＜0.05),而rs8122和rs34741656的基因型和等位基因频率在肥胖组与对照组之间差异均无统计学意义(P均＞0.05).肥胖组A-G-G单体型频率低于对照组(17％比20％,P＜0.05),而G-A-G单体型频率高于对照组(62.4％比58.9％,P＜0.05).多因素非条件logistic回归分析在调整性别、年龄、吸烟和饮酒后显示,rs1981529的AA基因型(OR:1.276,95％ CI:1.049 ～1.552,P＜0.05)和G-A-G单体型(OR:1.356,95％ CI:1.007 ～1.862,P＜0.05)是肥胖的独立危险因素.结论 STAMP2基因rs1981529的AA基因型可能与维吾尔族人群的中心性肥胖有关,G-A-G单体型可能是维吾尔族人群肥胖的易感标记.
목적 탐토전렬선륙차과막단백-2(STAMP2)기인다태성여유오이족인군중심성비반적상관성.방법 채취이류행병학조사위기출적병례대조연구방법,선택2332례유오이족인위연구대상,기중1455례중심성비반자작위비반조,기여877례비비반자작위대조조.측서사사STAMP2기인변이위점후,이TaqMan-PCR기술대대표성변이위점진행기인분형,분석STAMP2기인다태성여유오이족중심성비반적상관성.결과 측서발현20개변이,포괄14개신발현적변이위점.비반조STAMP2기인rs1981529적AA기인형빈솔고우대조조(67.6％비62.8％,P＜0.05),이rs8122화rs34741656적기인형화등위기인빈솔재비반조여대조조지간차이균무통계학의의(P균＞0.05).비반조A-G-G단체형빈솔저우대조조(17％비20％,P＜0.05),이G-A-G단체형빈솔고우대조조(62.4％비58.9％,P＜0.05).다인소비조건logistic회귀분석재조정성별、년령、흡연화음주후현시,rs1981529적AA기인형(OR:1.276,95％ CI:1.049 ～1.552,P＜0.05)화G-A-G단체형(OR:1.356,95％ CI:1.007 ～1.862,P＜0.05)시비반적독립위험인소.결론 STAMP2기인rs1981529적AA기인형가능여유오이족인군적중심성비반유관,G-A-G단체형가능시유오이족인군비반적역감표기.
Objective To investigate the association between genetic variations of the six transmembrance protein of prostate 2 (STAMP2) and obesity in Xinjiang Uygur population.Methods A total of 2332 Uygur subjects (1455 obesity and 877 non-obesity control subjects) were included in this casecontrol study based on epidemiological survey.Genetic variations of STAMP2 gene functional region were sequenced.The representative variations selected were genotyped by TaqMan-PCR method.Results Twenty genetic variations,including 14 novel variations,were identified.The genotype distributions of the control group and obesity group were in the Hardy-Weinberg equilibrium (both P ＞ 0.05).The frequency of AA of rs1981529 (67.6％ vs.62.8％,P ＜0.05) and the frequency of G-A-G haplotype (62.4％ vs.58.9％,P＜0.05) in obesity group were significantly higher than that in controls while the frequency of A-G-G haplotype was significantly lower in the obesity patients than that in the control group (17％ vs.20％,P ＜0.05).After adjusting age,sex,smoking and drinking,logistic regression analysis showed that the AA genotype of rs1981529 (OR:1.276,95％ CI:1.049-1.552;P ＜ 0.05) and the G-A-G haplotype (OR:1.356,95％ CI:1.007-1.862,P ＜ 0.05) were the independent risk factors for obesity in this cohort.Conclusion The AA genotype of rs1981529 and G-A-G haplotype are associated with obesity in Uygur population of Xinjiang.