中华心血管病杂志
中華心血管病雜誌
중화심혈관병잡지
Chinese Journal of Cardiology
2012年
12期
1056-1061
,共6页
孟存良%齐晓勇%李英肖%党懿%刘惠良%王天红%袁华兵%倪艳辉%刘光%武振朝%郜利会
孟存良%齊曉勇%李英肖%黨懿%劉惠良%王天紅%袁華兵%倪豔輝%劉光%武振朝%郜利會
맹존량%제효용%리영초%당의%류혜량%왕천홍%원화병%예염휘%류광%무진조%고리회
主动脉,腹%肌细胞,平滑肌%细胞增殖%白细胞介素8
主動脈,腹%肌細胞,平滑肌%細胞增殖%白細胞介素8
주동맥,복%기세포,평활기%세포증식%백세포개소8
Aorta,abdominal%Myocytes,smooth muscle%Cell proliferation%Interleukin-8
目的 观察球囊损伤腹主动脉后白细胞介素-8(IL-8)水平变化对兔腹主动脉狭窄和平滑肌细胞增殖的影响.方法 新西兰大白兔36只,随机分为实验组、IL-8单克隆抗体治疗组(治疗组)、对照组,每组12只.实验组和治疗组动物穿刺股动脉,送人球囊拉伤腹主动脉.治疗组动物通过耳缘静脉注射IL-8单克隆抗体进行干预.对照组动物只进行股动脉穿刺和留置鞘管,不进行球囊扩张拉伤腹主动脉.分别于实验前和实验后4h、1d、3d、1周、2周、4周取外周静脉血4 ml,检测血清IL-8水平.4周后处死动物,取腹主动脉进行病理组织学检查,应用光学显微镜和计算机病理图像分析系统分析测定以下指标:内膜中膜厚度、面积,计算血管狭窄程度.增殖细胞核抗原(PCNA)染色分析后,测定每40倍视野中PCNA阳性细胞,计算阳性细胞数和阳性细胞百分率.结果 实验组动物球囊损伤4h后血清IL-8水平即开始升高,术后1d达到峰值,持续增高4周,且均明显高于治疗组和对照组(P均＜0.01).治疗组和对照组动物IL-8水平无上述变化规律.实验组PCNA阳性细胞比例明显高于治疗组与对照组(P均＜0.05),治疗组明显高于对照组(P＜0.05).相关分析显示IL-8水平与PCNA阳性细胞数呈正相关,相关系数r=0.864(P ＜0.01).实验组动物腹主动脉明显狭窄,组织学检查内膜面积和中膜面积、血管狭窄程度等指标大于治疗组和对照组(P均＜0.01),治疗组管腔有所狭窄,对照组动物管腔无明显变化,治疗组与对照组比较差异无统计学意义.相关分析显示,IL-8水平与PNCA阳性细胞数、内膜厚度、内膜面积、中膜厚度、中膜面积等呈正相关(相关系数r分别为0.684,0.894,0.783,0.801,0.912,P均＜0.01).结论 球囊损伤引起IL-8水平高表达,导致腹主动脉平滑肌细胞增殖以及动脉管腔狭窄.IL-8水平与动脉狭窄程度呈正相关,IL-8是发生腹主动脉狭窄的独立危险因素.
目的 觀察毬囊損傷腹主動脈後白細胞介素-8(IL-8)水平變化對兔腹主動脈狹窄和平滑肌細胞增殖的影響.方法 新西蘭大白兔36隻,隨機分為實驗組、IL-8單剋隆抗體治療組(治療組)、對照組,每組12隻.實驗組和治療組動物穿刺股動脈,送人毬囊拉傷腹主動脈.治療組動物通過耳緣靜脈註射IL-8單剋隆抗體進行榦預.對照組動物隻進行股動脈穿刺和留置鞘管,不進行毬囊擴張拉傷腹主動脈.分彆于實驗前和實驗後4h、1d、3d、1週、2週、4週取外週靜脈血4 ml,檢測血清IL-8水平.4週後處死動物,取腹主動脈進行病理組織學檢查,應用光學顯微鏡和計算機病理圖像分析繫統分析測定以下指標:內膜中膜厚度、麵積,計算血管狹窄程度.增殖細胞覈抗原(PCNA)染色分析後,測定每40倍視野中PCNA暘性細胞,計算暘性細胞數和暘性細胞百分率.結果 實驗組動物毬囊損傷4h後血清IL-8水平即開始升高,術後1d達到峰值,持續增高4週,且均明顯高于治療組和對照組(P均＜0.01).治療組和對照組動物IL-8水平無上述變化規律.實驗組PCNA暘性細胞比例明顯高于治療組與對照組(P均＜0.05),治療組明顯高于對照組(P＜0.05).相關分析顯示IL-8水平與PCNA暘性細胞數呈正相關,相關繫數r=0.864(P ＜0.01).實驗組動物腹主動脈明顯狹窄,組織學檢查內膜麵積和中膜麵積、血管狹窄程度等指標大于治療組和對照組(P均＜0.01),治療組管腔有所狹窄,對照組動物管腔無明顯變化,治療組與對照組比較差異無統計學意義.相關分析顯示,IL-8水平與PNCA暘性細胞數、內膜厚度、內膜麵積、中膜厚度、中膜麵積等呈正相關(相關繫數r分彆為0.684,0.894,0.783,0.801,0.912,P均＜0.01).結論 毬囊損傷引起IL-8水平高錶達,導緻腹主動脈平滑肌細胞增殖以及動脈管腔狹窄.IL-8水平與動脈狹窄程度呈正相關,IL-8是髮生腹主動脈狹窄的獨立危險因素.
목적 관찰구낭손상복주동맥후백세포개소-8(IL-8)수평변화대토복주동맥협착화평활기세포증식적영향.방법 신서란대백토36지,수궤분위실험조、IL-8단극륭항체치료조(치료조)、대조조,매조12지.실험조화치료조동물천자고동맥,송인구낭랍상복주동맥.치료조동물통과이연정맥주사IL-8단극륭항체진행간예.대조조동물지진행고동맥천자화류치초관,불진행구낭확장랍상복주동맥.분별우실험전화실험후4h、1d、3d、1주、2주、4주취외주정맥혈4 ml,검측혈청IL-8수평.4주후처사동물,취복주동맥진행병리조직학검사,응용광학현미경화계산궤병리도상분석계통분석측정이하지표:내막중막후도、면적,계산혈관협착정도.증식세포핵항원(PCNA)염색분석후,측정매40배시야중PCNA양성세포,계산양성세포수화양성세포백분솔.결과 실험조동물구낭손상4h후혈청IL-8수평즉개시승고,술후1d체도봉치,지속증고4주,차균명현고우치료조화대조조(P균＜0.01).치료조화대조조동물IL-8수평무상술변화규률.실험조PCNA양성세포비례명현고우치료조여대조조(P균＜0.05),치료조명현고우대조조(P＜0.05).상관분석현시IL-8수평여PCNA양성세포수정정상관,상관계수r=0.864(P ＜0.01).실험조동물복주동맥명현협착,조직학검사내막면적화중막면적、혈관협착정도등지표대우치료조화대조조(P균＜0.01),치료조관강유소협착,대조조동물관강무명현변화,치료조여대조조비교차이무통계학의의.상관분석현시,IL-8수평여PNCA양성세포수、내막후도、내막면적、중막후도、중막면적등정정상관(상관계수r분별위0.684,0.894,0.783,0.801,0.912,P균＜0.01).결론 구낭손상인기IL-8수평고표체,도치복주동맥평활기세포증식이급동맥관강협착.IL-8수평여동맥협착정도정정상관,IL-8시발생복주동맥협착적독립위험인소.
Objective To observe the effects of interleukin-8 monoclonal antibody on smooth muscle cell proliferation and balloon inflation-induced abdominal aorta stenosis in rabbits.Methods Thirtysix New Zealand white rabbits were randomly assigned to balloon inflation group (group A,n =12),intedeukin-8 monoclonal antibody pre-treated rabbits (2 mg/kg for 3 days before balloon inflation,group B,n =12) and sham-operated control group (group C,n =12).Peripheral blood was collected before experiment and at 4 h,1,3,7,14,and 28 days post balloon inflation or sham operation and the levels of IL-8 were measured by enzyme linked immunosorbent assay (ELISA).The ratio of positive and negative masculine cells in the high power microscopic field was determined in proliferating cell nuclear antigen (PCNA) stained slide.Histopathologic examination was performed in abdominal aorta and luminal area,intima and tunica media area were measured.Results Plasma interleukin-8 began to rise at 4 h and peaked at 1 day and remained increased up to 28 days after balloon inflation in rabbits of group A,plasma interleukin-8 level in group A was significantly higher than in group B and C at 4 h and thereafter post operation.The ratio of positive and negative masculine cells was significantly increased in group A compared to group C and was significantly lower in group B than in group A.Abdominal aorta stenosis,luminal area,intima and tunica media area were significantly reduced in group B than in group A.Correlation analysis indicated that there were positive relations between plasma IL-8 level and intima thickness,area of intima and tunica medias,respectively (r =0.894,0.783,0.801,0.912,all P ＜0.01).Conclusions Plasma IL-8 level is increased in this abdominal aorta stenosis model and is positively correlated to the severity of abdominal aorta stenosis.IL-8 monoclonal antibody could significantly reduce abdominal aorta stenosis in this abdominal aorta stenosis model.
