CAJ | 학술논문

目的观察活化态C激酶1受体(RACK1)在冠心病患者外周血单个核细胞及冠状动脉粥样硬化斑块内的表达情况.方法选取住院冠心病患者70例,其中急性冠状动脉综合征(ACS)组41例、稳定性心绞痛(SAP)组29例,30名健康者作为对照组,用实时荧光定量PCR法测定外周血单个核细胞RACK1基因表达,免疫印迹法测定RACK1蛋白表达；选择尸体解剖冠状动脉粥样硬化病变标本17例及正常冠状动脉组织6例,免疫组织化学法测定RACK1在冠状动脉组织及斑块内的表达.结果 (1) ACS组患者外周血单个核细胞RACK1基因表达明显高于SAP组(18.71±5.45比12.18 ±4.14,P＜0.05),SAP组明显高于对照组(12.18 ±4.14比3.65 ±1.57,P＜0.05).(2) ACS组患者外周血单个核细胞RACK1蛋白表达明显高于SAP组(P＜0.05),SAP组明显高于对照组(P＜0.05).(3)冠状动脉粥样硬化斑块抗RACK1染色着色明显,其中不稳定斑块尤著,阳性染色主要位于泡沫细胞、炎性细胞、血管平滑肌细胞及内皮细胞,对照的正常冠状动脉仅于血管平滑肌细胞及内皮细胞少量表达.结论冠心病患者外周血单个核细胞RACK1基因及蛋白表达明显高于正常对照组,其中以ACS患者为著；冠状动脉粥样硬化斑块RACK1蛋白表达明显增加,其中不稳定斑块尤为明显,提示RACK1可能在冠心病发生和进展中发挥重要作用.
목적 관찰활화태C격매1수체(RACK1)재관심병환자외주혈단개핵세포급관상동맥죽양경화반괴내적표체정황.방법 선취주원관심병환자70례,기중급성관상동맥종합정(ACS)조41례、은정성심교통(SAP)조29례,30명건강자작위대조조,용실시형광정량PCR법측정외주혈단개핵세포RACK1기인표체,면역인적법측정RACK1단백표체；선택시체해부관상동맥죽양경화병변표본17례급정상관상동맥조직6례,면역조직화학법측정RACK1재관상동맥조직급반괴내적표체.결과 (1) ACS조환자외주혈단개핵세포RACK1기인표체명현고우SAP조(18.71±5.45비12.18 ±4.14,P＜0.05),SAP조명현고우대조조(12.18 ±4.14비3.65 ±1.57,P＜0.05).(2) ACS조환자외주혈단개핵세포RACK1단백표체명현고우SAP조(P＜0.05),SAP조명현고우대조조(P＜0.05).(3)관상동맥죽양경화반괴항RACK1염색착색명현,기중불은정반괴우저,양성염색주요위우포말세포、염성세포、혈관평활기세포급내피세포,대조적정상관상동맥부우혈관평활기세포급내피세포소량표체.결론 관심병환자외주혈단개핵세포RACK1기인급단백표체명현고우정상대조조,기중이ACS환자위저；관상동맥죽양경화반괴RACK1단백표체명현증가,기중불은정반괴우위명현,제시RACK1가능재관심병발생화진전중발휘중요작용.
Objective To observe the expression and clinical implication of receptor for activated C kinase 1 (RACK1) in mononuclear cells and coronary atherosclerotic plaques from patients with coronary artery disease.Methods mRNA and protein expressions of RACK1 were detected in mononuclear cells from 29 patients with stable angina pectoris (SAP),41 patients with acute coronary syndrome(ACS) and 30 healthy volunteers.RACK1 protein expression was also detected by immunohistochemistry in 17 coronary atherosclerotic plaques and 6 normal autopsy coronary samples.Results (1) mRNA expression of RACK1 was significantly upregulated in mononuclear cells from patients with ACS compared with those from patients with SAP (18.71 ± 5.45 vs.12.18 ± 4.14,P ＜ 0.05),and the latter was also significantly higher than in healthy controls (12.18 ± 4.14 vs.3.65 ± 1.57,P ＜ 0.05).(2) Similar changes were observed for protein expression of RACK1 for the three groups.(3) Increased expression of RACK1 was found in atherosclerotic plaques,especially in unstable plaques,positive RACK1 stain was evidenced in foam cells,inflammatory cells,smooth muscle cells and endothelial cells.Conclusions The expression of RACK1 is significantly upregulated in mononuclear cells from patients with coronary artery disease,especially in patients with ACS,and in coronary atherosclerotic plaques,especially in unstable plaques.Our results thus suggest that RACK1 might play an important role in the development and progression of coronary artery disease.