CAJ | 학술논문

目的观察冠状动脉慢血流(CSF)患者循环内皮祖细胞(EPC)数量与CSF之间的关系,探讨CSF发病的可能机制.方法选择冠状动脉造影结果正常和CSF患者各30例,采用密度梯度离心法从外周血获取单个核细胞,通过FITC标记荆豆凝集素和DiI标记的乙酰化低密度脂蛋白双染色、倒置荧光显微镜(200倍视野)鉴定EPC并对EPC进行计数.应用t检验和卡方检验比较两组患者临床资料的差异,并采用logistic回归分析法对相关因素进行分析.结果两组患者的年龄、性别、高血压、糖尿病、吸烟史所占比例及血脂水平差异均无统计学意义,CSF患者外周血EPC数量明显少于正常对照组(35.7±5.9比53,2±5.9,t=10.3,P＜0.01).logistic回归分析显示,性别、年龄、吸烟史、高血压史、糖尿病史及血脂水平等变量都与CSF无明显相关性；而随着EPC数目增加,CSF发生概率减少(OR=0.424,95％ CI0.358～0.621,P＜0.01).结论循环EPC数量减少可能是CSF独立危险因素之一,与CSF发病有关.
목적 관찰관상동맥만혈류(CSF)환자순배내피조세포(EPC)수량여CSF지간적관계,탐토CSF발병적가능궤제.방법 선택관상동맥조영결과정상화CSF환자각30례,채용밀도제도리심법종외주혈획취단개핵세포,통과FITC표기형두응집소화DiI표기적을선화저밀도지단백쌍염색、도치형광현미경(200배시야)감정EPC병대EPC진행계수.응용t검험화잡방검험비교량조환자림상자료적차이,병채용logistic회귀분석법대상관인소진행분석.결과 량조환자적년령、성별、고혈압、당뇨병、흡연사소점비례급혈지수평차이균무통계학의의,CSF환자외주혈EPC수량명현소우정상대조조(35.7±5.9비53,2±5.9,t=10.3,P＜0.01).logistic회귀분석현시,성별、년령、흡연사、고혈압사、당뇨병사급혈지수평등변량도여CSF무명현상관성；이수착EPC수목증가,CSF발생개솔감소(OR=0.424,95％ CI0.358～0.621,P＜0.01).결론 순배EPC수량감소가능시CSF독립위험인소지일,여CSF발병유관.
Objective To explore if reduced number of circulating endothelial progenitor cells (EPCs) is a risk factor for patients with coronary slow flow (CSF).Methods Thirty patients with CSF and 30 age and gender matched control subjects with normal coronary angiography were included in the study.Mononuclear cells were isolated from peripheral blood by Ficoll density gradient centrifugation and plated on fibronectin-coated culture dishes.EPCs were characterized as adherent cells double positive for DiI-AcLDLuptake and lectin-binding by converted fluorescence microscope (× 200).Results Smoking,diabetes mellitus,hypertension and the levels of plasma lipoprotein profile were similar between the two groups (all P ＞ 0.05).The number of EPCs was significantly lower in patients with CSF compared with control subjects (35.7 ± 5.9 vs.53.2 ± 5.9,P ＜ 0.01).TIMI frame counts was correlated with circulating EPCs number (OR =0.424,95％ CI 0.358-0.621,P ＜ 0.01) and not associated with gender,age,smoking,diabetes mellitus,hypertension and the levels of plasma lipoprotein profile.Conclusion Decreased circulating EPCs is an independent risk factor for CSF.