CAJ | 학술논문

目的探讨小鼠骨髓间充质干细胞(BMSC)各亚群在心肌修复中的作用.方法小鼠心脏干细胞表面分化抗原CD45、CD31检测BMSC后,以其阴性及阳性为标准分选得到4个细胞亚群,分别为SCA-1 +/CD45 +/CD31+、SCA-1 +/CD45+/CD31-、SCA-1+/CD45-/CD31-和SCA-1+/CD45-/CD31+亚群.将4个细胞亚群及未分选群分别经尾静脉注入心肌梗死小鼠体内,于注射后48 h完成心脏彩超心功能测定及小动物活体成像检查,并行心脏梗死原位C-kit(心肌干细胞标志抗原之一)免疫组织化学、苏木素-伊红染色及原位免疫组织化学心肌特异抗原检测.结果心脏彩超提示SCA-1 +/CD45 +/CD31+细胞亚群注入心肌梗死小鼠体内后48 h,其左心室收缩末期直径和舒张末期直径注入前后的差值均明显小于注入其余细胞亚群及未分选细胞群的小鼠(P均＜0.05),而左心室射血分数、短轴缩短率注入前后的差值则均高于注入其余细胞亚群及未分选细胞群的小鼠(P均＜0.05).小动物活体成像显示SCA-1 +/CD45 +/CD31+细胞亚群注入心肌梗死小鼠体内后48 h,其荧光强度高于注入其余各细胞亚群及未分选群的小鼠(P均＜0.05).各细胞亚群及未分选群干细胞注入心肌梗死小鼠后48 h心肌局部均可见心肌干细胞大量增加.各细胞亚群动员心肌干细胞的能力为SCA-l+/CD45+/CD31+细胞亚群＞ SCA-1 +/CD45-/CD31+细胞亚群＞SCA-1+/CD45-/CD31-细胞亚群＞ SCA-1 +/CD45 +/CD31-细胞亚群.SCA-1+/CD45+/CD31+细胞亚群注入心肌梗死小鼠后48 h,梗死心肌局部可见其向心肌样细胞分化.结论 BMSC SCA-1 +/CD45 +/CD31+细胞亚群可改善心肌梗死小鼠心功能,其抗凋亡及动员自身心肌干细胞的能力优于其他细胞亚群及未分选群,并可分化为心肌样细胞.
목적 탐토소서골수간충질간세포(BMSC)각아군재심기수복중적작용.방법 소서심장간세포표면분화항원CD45、CD31검측BMSC후,이기음성급양성위표준분선득도4개세포아군,분별위SCA-1 +/CD45 +/CD31+、SCA-1 +/CD45+/CD31-、SCA-1+/CD45-/CD31-화SCA-1+/CD45-/CD31+아군.장4개세포아군급미분선군분별경미정맥주입심기경사소서체내,우주사후48 h완성심장채초심공능측정급소동물활체성상검사,병행심장경사원위C-kit(심기간세포표지항원지일)면역조직화학、소목소-이홍염색급원위면역조직화학심기특이항원검측.결과 심장채초제시SCA-1 +/CD45 +/CD31+세포아군주입심기경사소서체내후48 h,기좌심실수축말기직경화서장말기직경주입전후적차치균명현소우주입기여세포아군급미분선세포군적소서(P균＜0.05),이좌심실사혈분수、단축축단솔주입전후적차치칙균고우주입기여세포아군급미분선세포군적소서(P균＜0.05).소동물활체성상현시SCA-1 +/CD45 +/CD31+세포아군주입심기경사소서체내후48 h,기형광강도고우주입기여각세포아군급미분선군적소서(P균＜0.05).각세포아군급미분선군간세포주입심기경사소서후48 h심기국부균가견심기간세포대량증가.각세포아군동원심기간세포적능력위SCA-l+/CD45+/CD31+세포아군＞ SCA-1 +/CD45-/CD31+세포아군＞SCA-1+/CD45-/CD31-세포아군＞ SCA-1 +/CD45 +/CD31-세포아군.SCA-1+/CD45+/CD31+세포아군주입심기경사소서후48 h,경사심기국부가견기향심기양세포분화.결론 BMSC SCA-1 +/CD45 +/CD31+세포아군가개선심기경사소서심공능,기항조망급동원자신심기간세포적능력우우기타세포아군급미분선군,병가분화위심기양세포.
Objective To search for the bone mesenchymal stem cell (MSC) subgroup which might be more effective on repairing myocardial damage.Methods In this experiment,four MSC subgroups were defined based on the surface differentiation antigen detection of mouse bone mesenchymal stem cells (mBMSCs):SCA-1 +/CD45 +/CD31 +,SCA-1 +/CD45 +/CD31-,SCA-1 +/CD45-/CD31-and SCA-1 +/CD45-/CD31 +.These subgroup cells and unselected mBMSCs were injected into infarcted mouse via tail vein.Echocardiographic heart function measurement and in vivo DiR-labeled stem cells imaging were performed at 48 h after injection.In situ C-kit (a flag antigen of cardiac stem cells) and cardiac-specific differentiation antigen immunohistochemistry detection was made in the infarcted myocardium.Results The capacity of the SCA-1 +/CD45 +/CD31 + cells on improving heart function was significantly higher than other cell groups(all P ＜ 0.05).In vivo imaging showed that the mean fluorescence intensity of the SCA-1 +/CD45 +/CD31 + cells was also higher than other cell groups (all P ＜ 0.05).Number of cardiac stem cells in the infracted myocardium was significantly increased after the injection of all subgroup cells and unsorted mBMSCs cells for 48 h compared untreated infracted myocardium.The capacity of mobilizing cardiac stem cells is as follows:SCA-1 +/CD45 +/CD31 + ＞ SCA-1 +/CD45-/CD31 + ＞ SCA-1 +/CD45-/CD31-＞ SCA-1+/CD45+/CD31-.Conclusion The SCA-1+/CD45+/CD31+ subgroups of mBMSCs exhibites the highest capacity to improve cardiac function after myocardial infarction and to mobilize autologous cardiac stem cells compared with other mBMSCs subgroups and unsorted mBMSCs cells.