中华心血管病杂志
中華心血管病雜誌
중화심혈관병잡지
Chinese Journal of Cardiology
2013年
5期
399-405
,共7页
高静%毛用敏%丛洪良%刘祎%张楠%陈倩%刘婷%崔让庄
高靜%毛用敏%叢洪良%劉祎%張楠%陳倩%劉婷%崔讓莊
고정%모용민%총홍량%류의%장남%진천%류정%최양장
冠状动脉疾病%核苷转运蛋白质类%多态性,单核苷酸
冠狀動脈疾病%覈苷轉運蛋白質類%多態性,單覈苷痠
관상동맥질병%핵감전운단백질류%다태성,단핵감산
Coronary disease%Nucleoside transfer proteins%Polymorphism,sigle nucleotide
目的 研究天津地区人群胆固醇酯转运蛋白(cholesteryl ester transfer protein,CETP)-629C/A基因多态性、血清CETP含量、脂蛋白代谢及与冠心病发病风险相互之间的关系.方法 采用以医院为基础的成组病例对照研究方法,研究对象来自2010年10月至2011年10月天津市胸科医院心内科住院并接受冠状动脉造影检查的患者,天津地区汉族人群,根据冠状动脉造影结果分为冠心病组429例,正常对照组275例.采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法,检测CETP-629C/A基因型,同时采用ELISA方法测定其血清CETP含量,分析-629C/A基因多态性、血清CETP含量、脂蛋白代谢及与冠心病发病风险相互之间的关系.结果 (1)天津地区汉族人群CETP基因-629位点A等位基因频率为0.408,明显低于国内其他地区及国外人群(0.479 ～0.701,P＜0.05).(2)突变型纯合子AA基因型血清CETP浓度低于野生型纯合子CC基因型(P＞0.05),而高密度脂蛋白胆固醇(HDL-C)水平显著增高(P＜0.05).(3)血清CETP浓度与HDL-C水平呈负向变化趋势,其相关性未达到统计学意义(P＞0.05).(4)冠心病组及对照组-629C/A各基因型和等位基因频率分布差异有统计学意义(P＜0.001),携带CA/AA基因型和携带A等位基因个体发生冠心病的危险性增加,OR(95％ CI)值分别为4.627(3.163 ～6.769)、8.779 (4.799 ～ 16.059)和3.173(2.453 ～4.104),调整了年龄、性别、体质指数、吸烟、高血压、糖尿病和血脂代谢障碍等混杂因素后这种关联仍然存在.-629C/A基因多态性与冠状动脉血管病变程度不相关(x2=3.588,P＞0.05).结论 天津地区汉族人群CETP-629C/A基因型和等位基因频率分布与国内其他地区及国外人群有明显差异；-629C→A突变与冠心病的发病风险密切相关,变异基因型(CA和AA)是冠心病发病独立危险因素,-629A等位基因可能是天津地区汉族人群冠心病遗传易感基因；-629C→A突变导致CETP浓度降低、血清HDL-C水平的升高不能完全改变个体对冠心病易患性.
目的 研究天津地區人群膽固醇酯轉運蛋白(cholesteryl ester transfer protein,CETP)-629C/A基因多態性、血清CETP含量、脂蛋白代謝及與冠心病髮病風險相互之間的關繫.方法 採用以醫院為基礎的成組病例對照研究方法,研究對象來自2010年10月至2011年10月天津市胸科醫院心內科住院併接受冠狀動脈造影檢查的患者,天津地區漢族人群,根據冠狀動脈造影結果分為冠心病組429例,正常對照組275例.採用聚閤酶鏈反應-限製性片段長度多態性(PCR-RFLP)方法,檢測CETP-629C/A基因型,同時採用ELISA方法測定其血清CETP含量,分析-629C/A基因多態性、血清CETP含量、脂蛋白代謝及與冠心病髮病風險相互之間的關繫.結果 (1)天津地區漢族人群CETP基因-629位點A等位基因頻率為0.408,明顯低于國內其他地區及國外人群(0.479 ～0.701,P＜0.05).(2)突變型純閤子AA基因型血清CETP濃度低于野生型純閤子CC基因型(P＞0.05),而高密度脂蛋白膽固醇(HDL-C)水平顯著增高(P＜0.05).(3)血清CETP濃度與HDL-C水平呈負嚮變化趨勢,其相關性未達到統計學意義(P＞0.05).(4)冠心病組及對照組-629C/A各基因型和等位基因頻率分佈差異有統計學意義(P＜0.001),攜帶CA/AA基因型和攜帶A等位基因箇體髮生冠心病的危險性增加,OR(95％ CI)值分彆為4.627(3.163 ～6.769)、8.779 (4.799 ～ 16.059)和3.173(2.453 ～4.104),調整瞭年齡、性彆、體質指數、吸煙、高血壓、糖尿病和血脂代謝障礙等混雜因素後這種關聯仍然存在.-629C/A基因多態性與冠狀動脈血管病變程度不相關(x2=3.588,P＞0.05).結論 天津地區漢族人群CETP-629C/A基因型和等位基因頻率分佈與國內其他地區及國外人群有明顯差異；-629C→A突變與冠心病的髮病風險密切相關,變異基因型(CA和AA)是冠心病髮病獨立危險因素,-629A等位基因可能是天津地區漢族人群冠心病遺傳易感基因；-629C→A突變導緻CETP濃度降低、血清HDL-C水平的升高不能完全改變箇體對冠心病易患性.
목적 연구천진지구인군담고순지전운단백(cholesteryl ester transfer protein,CETP)-629C/A기인다태성、혈청CETP함량、지단백대사급여관심병발병풍험상호지간적관계.방법 채용이의원위기출적성조병례대조연구방법,연구대상래자2010년10월지2011년10월천진시흉과의원심내과주원병접수관상동맥조영검사적환자,천진지구한족인군,근거관상동맥조영결과분위관심병조429례,정상대조조275례.채용취합매련반응-한제성편단장도다태성(PCR-RFLP)방법,검측CETP-629C/A기인형,동시채용ELISA방법측정기혈청CETP함량,분석-629C/A기인다태성、혈청CETP함량、지단백대사급여관심병발병풍험상호지간적관계.결과 (1)천진지구한족인군CETP기인-629위점A등위기인빈솔위0.408,명현저우국내기타지구급국외인군(0.479 ～0.701,P＜0.05).(2)돌변형순합자AA기인형혈청CETP농도저우야생형순합자CC기인형(P＞0.05),이고밀도지단백담고순(HDL-C)수평현저증고(P＜0.05).(3)혈청CETP농도여HDL-C수평정부향변화추세,기상관성미체도통계학의의(P＞0.05).(4)관심병조급대조조-629C/A각기인형화등위기인빈솔분포차이유통계학의의(P＜0.001),휴대CA/AA기인형화휴대A등위기인개체발생관심병적위험성증가,OR(95％ CI)치분별위4.627(3.163 ～6.769)、8.779 (4.799 ～ 16.059)화3.173(2.453 ～4.104),조정료년령、성별、체질지수、흡연、고혈압、당뇨병화혈지대사장애등혼잡인소후저충관련잉연존재.-629C/A기인다태성여관상동맥혈관병변정도불상관(x2=3.588,P＞0.05).결론 천진지구한족인군CETP-629C/A기인형화등위기인빈솔분포여국내기타지구급국외인군유명현차이；-629C→A돌변여관심병적발병풍험밀절상관,변이기인형(CA화AA)시관심병발병독립위험인소,-629A등위기인가능시천진지구한족인군관심병유전역감기인；-629C→A돌변도치CETP농도강저、혈청HDL-C수평적승고불능완전개변개체대관심병역환성.
Objective To investigate the relationship between the-629C/A polymorphism in the promoter region of the CETP gene,serum Levels,lipid metabolism,and coronary heart disease (CHD) among Tianjin Han Chinese population.Methods A hospital-based case-control study was conducted in Tianjin Chest Hospital from 2010 October to 2011 October.The subjects underwent angiography were divided into a case group (n =429) and a control group (n =275).The CETP gene promoter polymorphism at position-629 was determined by restricted fragment length polymorphism using the polymerase chain reaction (PCR-RFLP) method.The serum CETP levels was determined by enzyme-linked immunosorbent assay (ELISA) method.Results (1) The frequencies of-629A allele in Tianjin Han Chinese population was 0.408,significantly lowever than that in other domestic and foreign populations (0.479-0.701,P ＜ 0.05).(2) Variant AA genotype showed reduced CETP levels (P ＞ 0.05) and higher HDL-C levels (P ＜0.05),compared to wild CC genotype.(3) Although there was a negative trend correlation between serum CETP and HDL-C levels,it did not reach statistical significance (P ＞ 0.05).(4) There were significant differences in the frequencies of CETP gene-629 genotype and allele between the two groups (P ＜ 0.001),carries with CA/AA genotype and A allele showed higher risk of CHD,OR (95％ CI) values were 4.627 (3.163-6.769) 、8.779 (4.799-16.059) and 3.173 (2.453-4.104) respectively.There was no relationship between CETP-629C/A polymorphism and coronary artery stenosis degree (x2 =3.588,P =0.166).Conclusion The frequencies of CETP gene-629 genotype and allele in the Tianjin Han Chinese population was significantly different from that in Other domestic and foreign populations.Variant AA genotype,which showed reduced CETP levels and higher HDL-C levels,is paradoxically associated with increased risk of CHD.Thus,CETP gene variation may affect coronary risk apart from the level of HDL-C.
