中华胸心血管外科杂志
中華胸心血管外科雜誌
중화흉심혈관외과잡지
Chinese Journal of Thoracic and Cardiovascular Surgery
2013年
5期
305-307
,共3页
杨欣%张治%胡静雯%邱满堂%徐磊%钱以淳%游庆军%尹荣%许林
楊訢%張治%鬍靜雯%邱滿堂%徐磊%錢以淳%遊慶軍%尹榮%許林
양흔%장치%호정문%구만당%서뢰%전이순%유경군%윤영%허림
非小细胞腺癌%肺%转移%磷脂酰肌醇蛋白聚糖-5%GPC5
非小細胞腺癌%肺%轉移%燐脂酰肌醇蛋白聚糖-5%GPC5
비소세포선암%폐%전이%린지선기순단백취당-5%GPC5
Non-small cell lung adenocarcinoma%Lung%Metastasis%Glypican-5%GPC5
目的 探讨磷脂酰肌醇蛋白聚糖-5(GPC5)在非小细胞肺腺癌中的表达水平及其临床意义.方法 应用Real-time PCR的方法检测134例非小细胞肺腺癌组织及其对应的正常肺组织中的GPC5基因的表达情况.应用免疫组化方法验证GPC5蛋白在人肺腺癌组织芯片(包含75对腺癌组织标本)中的表达情况.结果 (1) GPC5 mRNA表达水平在非小细胞肺腺癌组织中较正常肺组织显著下降(P＜0.001).(2)有淋巴结转移组较无淋巴结转移组GPC5 mRNA和蛋白表达水平均显著降低(P＜0.05).(3) Spearman相关性分析显示GPC5 mRNA表达水平与淋巴结转移、病理分期和分化程度呈显著正相关(P＜0.05).(4) Kaplan-Meier生存曲线分析示GPC5蛋白表达与肺腺癌患者长期预后相关(P =0.036).结论 GPC5的表达水平与非小细胞肺腺癌的淋巴结转移密切相关,可作为评估预后的辅助标志物.
目的 探討燐脂酰肌醇蛋白聚糖-5(GPC5)在非小細胞肺腺癌中的錶達水平及其臨床意義.方法 應用Real-time PCR的方法檢測134例非小細胞肺腺癌組織及其對應的正常肺組織中的GPC5基因的錶達情況.應用免疫組化方法驗證GPC5蛋白在人肺腺癌組織芯片(包含75對腺癌組織標本)中的錶達情況.結果 (1) GPC5 mRNA錶達水平在非小細胞肺腺癌組織中較正常肺組織顯著下降(P＜0.001).(2)有淋巴結轉移組較無淋巴結轉移組GPC5 mRNA和蛋白錶達水平均顯著降低(P＜0.05).(3) Spearman相關性分析顯示GPC5 mRNA錶達水平與淋巴結轉移、病理分期和分化程度呈顯著正相關(P＜0.05).(4) Kaplan-Meier生存麯線分析示GPC5蛋白錶達與肺腺癌患者長期預後相關(P =0.036).結論 GPC5的錶達水平與非小細胞肺腺癌的淋巴結轉移密切相關,可作為評估預後的輔助標誌物.
목적 탐토린지선기순단백취당-5(GPC5)재비소세포폐선암중적표체수평급기림상의의.방법 응용Real-time PCR적방법검측134례비소세포폐선암조직급기대응적정상폐조직중적GPC5기인적표체정황.응용면역조화방법험증GPC5단백재인폐선암조직심편(포함75대선암조직표본)중적표체정황.결과 (1) GPC5 mRNA표체수평재비소세포폐선암조직중교정상폐조직현저하강(P＜0.001).(2)유림파결전이조교무림파결전이조GPC5 mRNA화단백표체수평균현저강저(P＜0.05).(3) Spearman상관성분석현시GPC5 mRNA표체수평여림파결전이、병리분기화분화정도정현저정상관(P＜0.05).(4) Kaplan-Meier생존곡선분석시GPC5단백표체여폐선암환자장기예후상관(P =0.036).결론 GPC5적표체수평여비소세포폐선암적림파결전이밀절상관,가작위평고예후적보조표지물.
Objective To investigate glypican-5 (GPC5) expression in Non-small cell lung adenocarcinoma and its clinical significance.Methods GPC5 was detected in 134 cases of Non-small cell lung adenocarcinoma and corresponding normal lung tissue by Real-time PCR,which was also validated in human lung adenocarcinoma tissue microarrays (TMA,containing 75 cases of lung adenocarcinoma tissue and adjacent tissue) by immunohistochemistry.Results (1) GPC5 mRNA expression level in Non-small cell lung adenocarcinoma was significantly lower than in matched normal lung tissue (P ＜ 0.001).(2)The expression levels of GPC5 mRNA and proteins in the lymph node metastasis group were all lower than those in the non-metastasis group(P ＜0.05).(3) Spearman analysis showed that the expression levels of GPC5 mRNA was significantly correlated with lymph node metastasis,TNM staging and differentiation.(4) The Kaplan-Meier survival analysis showed that the expressions of GPC5 proteins were related to the long-term prognosis of Non-small cell lung adenocarcinoma patients (P =0.036).Conclusion The expression levels of GPC5 were closely related with the lymph node metastasis of Non-small cell lung adenocarcinoma.It may be used as a marker of lung cancer diagnosis.
