中华胸心血管外科杂志
中華胸心血管外科雜誌
중화흉심혈관외과잡지
Chinese Journal of Thoracic and Cardiovascular Surgery
2014年
4期
242-246
,共5页
柳瑞军%过常发%陈昶%姜格宁
柳瑞軍%過常髮%陳昶%薑格寧
류서군%과상발%진창%강격저
成肌细胞,骨骼肌%细胞移植%血管内皮生长因子类
成肌細胞,骨骼肌%細胞移植%血管內皮生長因子類
성기세포,골격기%세포이식%혈관내피생장인자류
Myoblasts,skeletal%Cell transplantation%Vascular endothelial growth factors
目的 探讨骨骼肌成肌细胞移植治疗急性心肌梗死(AMI)时,是否存在幼稚Th淋巴细胞的Th17细胞(Th17)和调节性T细胞(Tregs)之间的Th 17/Treg失衡,血管内皮生长因子165(VEGF165)是否可缓解这种失衡.方法 C57BL/6雄性小鼠60只,3～4周龄,体质量0.016 ～0.020 kg.数字表法随机分为3组:AMI组、AMI-S组和AMI-Ⅴ组,每组20只.AMI组为对照组,AMI-S组为异体骨骼肌成肌细胞移植组,AMI-Ⅴ组为心肌梗死VEGF165治疗组.分别于异体骨骼肌成肌细胞移植(SMT)1、2、4和7天后,用流式细胞仪分析3组小鼠的Th17和Treg细胞比率.蛋白芯片和实时聚合酶链反应分别检测有关促炎细胞因子、调节性细胞因子、关键转录因子、ROR-γt mRNA以及Foxp3 mRNA的表达.结果 与AMI组相比较,1、2、4、7天后,AMI-S组的调节细胞百分比、有关细胞因子浓度和转录因子Foxp3mRNA低,AMI-Ⅴ组高;AMI-S组的Th17细胞百分比、有关细胞因子浓度和ROR-γt mRNA高,AMI-Ⅴ组低;AMI-S组中Th 17/Treg细胞比率显著增加,而AMI-Ⅴ组明显减少.结论 异体SMT后,炎性和免疫反应形成和发展过程中存在Th17/Treg失衡.转染VEGF165可减缓TH 17/Treg失衡.
目的 探討骨骼肌成肌細胞移植治療急性心肌梗死(AMI)時,是否存在幼稚Th淋巴細胞的Th17細胞(Th17)和調節性T細胞(Tregs)之間的Th 17/Treg失衡,血管內皮生長因子165(VEGF165)是否可緩解這種失衡.方法 C57BL/6雄性小鼠60隻,3～4週齡,體質量0.016 ～0.020 kg.數字錶法隨機分為3組:AMI組、AMI-S組和AMI-Ⅴ組,每組20隻.AMI組為對照組,AMI-S組為異體骨骼肌成肌細胞移植組,AMI-Ⅴ組為心肌梗死VEGF165治療組.分彆于異體骨骼肌成肌細胞移植(SMT)1、2、4和7天後,用流式細胞儀分析3組小鼠的Th17和Treg細胞比率.蛋白芯片和實時聚閤酶鏈反應分彆檢測有關促炎細胞因子、調節性細胞因子、關鍵轉錄因子、ROR-γt mRNA以及Foxp3 mRNA的錶達.結果 與AMI組相比較,1、2、4、7天後,AMI-S組的調節細胞百分比、有關細胞因子濃度和轉錄因子Foxp3mRNA低,AMI-Ⅴ組高;AMI-S組的Th17細胞百分比、有關細胞因子濃度和ROR-γt mRNA高,AMI-Ⅴ組低;AMI-S組中Th 17/Treg細胞比率顯著增加,而AMI-Ⅴ組明顯減少.結論 異體SMT後,炎性和免疫反應形成和髮展過程中存在Th17/Treg失衡.轉染VEGF165可減緩TH 17/Treg失衡.
목적 탐토골격기성기세포이식치료급성심기경사(AMI)시,시부존재유치Th림파세포적Th17세포(Th17)화조절성T세포(Tregs)지간적Th 17/Treg실형,혈관내피생장인자165(VEGF165)시부가완해저충실형.방법 C57BL/6웅성소서60지,3～4주령,체질량0.016 ～0.020 kg.수자표법수궤분위3조:AMI조、AMI-S조화AMI-Ⅴ조,매조20지.AMI조위대조조,AMI-S조위이체골격기성기세포이식조,AMI-Ⅴ조위심기경사VEGF165치료조.분별우이체골격기성기세포이식(SMT)1、2、4화7천후,용류식세포의분석3조소서적Th17화Treg세포비솔.단백심편화실시취합매련반응분별검측유관촉염세포인자、조절성세포인자、관건전록인자、ROR-γt mRNA이급Foxp3 mRNA적표체.결과 여AMI조상비교,1、2、4、7천후,AMI-S조적조절세포백분비、유관세포인자농도화전록인자Foxp3mRNA저,AMI-Ⅴ조고;AMI-S조적Th17세포백분비、유관세포인자농도화ROR-γt mRNA고,AMI-Ⅴ조저;AMI-S조중Th 17/Treg세포비솔현저증가,이AMI-Ⅴ조명현감소.결론 이체SMT후,염성화면역반응형성화발전과정중존재Th17/Treg실형.전염VEGF165가감완TH 17/Treg실형.
Objective To investigate whether Th17/Treg imbalance existed,and whether VEGF165 attenuated the imbalance in allogeneic skeletal myoblasts transplantation(allo-SMT) for acute myocardial infarction (AMI).Methods There were 60 C57BL/6 male mice,3 to 4 weeks old,weighting 0.016-0.020 kg.On the day 1,2,4,and 7 after allo-SMT,the percentages of Th17 and Treg cells,and the ratios of Th17/Treg cells were analyzed by FCM in AMI group,AMI-S group(alloSMT) and AMI-Ⅴ group(with VEGF165 treatment).Subsequently,related proinflammatory and regulatory cytokines and key transcription factors ROR-γt mRNA and Foxp3 mRNA expression were examined by Bio-plex and RT-PCR respectively.Results On the day 1,2,4,and 7 after allo-SMT,the percentage of Treg,related cytokines concentrations and transcript factor Foxp3 mRNA in AMI-S group were lower than those in AMI group,while the AMI-Ⅴ group were higher than those in AMI groups.However,the percentage of Th17 cells,related cytokines concentrations,and ROR-γt mRNA in AMI-S group were higher than those in AMI group; but the AMI-Ⅴ group were lower than those in AMI group.Apparently,compared with AMI group,the ratios of Th17/Treg significantly ascended in AMI-S group and decended in AMI-Ⅴ group.Conclusion Th17/Treg imbalance participated in the formation and development of inflammation and immune response after allo-SMT.However,transfected VEGF165 could relieve the severity of Th17/Treg imbalance.
