中华胸心血管外科杂志
中華胸心血管外科雜誌
중화흉심혈관외과잡지
Chinese Journal of Thoracic and Cardiovascular Surgery
2014年
7期
400-403
,共4页
气胸%肺大疱%成纤维细胞生长因子10%基质金属蛋白酶9%KL-6
氣胸%肺大皰%成纖維細胞生長因子10%基質金屬蛋白酶9%KL-6
기흉%폐대포%성섬유세포생장인자10%기질금속단백매9%KL-6
Pneumothorax%Bulla%Fibroblast growth factor 10%Matrix metalloproteinase 9%KL-6
目的 检测并比较细胞因子肺泡细胞表面抗原(KL)-6、成纤维细胞生长因子(FGF)-10、基质金属蛋白酶(MMP)-9在自发性气胸患者肺大疱和疱旁正常肺组织中的表达,探讨肺大疱形成过程中细胞因子的意义.方法 24例自发性气胸患者,采用免疫组织化学染色技术和酶联免疫吸附法(ELISA)检测肺大疱组织及疱旁正常肺组织中的KL-6、FGF-10及MMP-9表达水平,配对t检验分析结果.结果 免疫组化和ELISA结果均显示:肺大疱组织中KL-6和FGF-10的表达明显高于疱旁正常肺组织(P＜0.05),但MMP-9在两组间差异无统计学意义(P＞0.05).结论 自发性气胸患者肺大疱组织中KL-6和FGF-10的表达明显高于疱旁正常肺组织,MMP-9的表达未见明显差别.KL-6介导的肺纤维化和FGF-10表达异常导致的肺-支气管先天性发育异常可能与肺大疱的形成相关,MMP-9介导的炎性反应与肺大疱形成的相关性尚待进一步研究.
目的 檢測併比較細胞因子肺泡細胞錶麵抗原(KL)-6、成纖維細胞生長因子(FGF)-10、基質金屬蛋白酶(MMP)-9在自髮性氣胸患者肺大皰和皰徬正常肺組織中的錶達,探討肺大皰形成過程中細胞因子的意義.方法 24例自髮性氣胸患者,採用免疫組織化學染色技術和酶聯免疫吸附法(ELISA)檢測肺大皰組織及皰徬正常肺組織中的KL-6、FGF-10及MMP-9錶達水平,配對t檢驗分析結果.結果 免疫組化和ELISA結果均顯示:肺大皰組織中KL-6和FGF-10的錶達明顯高于皰徬正常肺組織(P＜0.05),但MMP-9在兩組間差異無統計學意義(P＞0.05).結論 自髮性氣胸患者肺大皰組織中KL-6和FGF-10的錶達明顯高于皰徬正常肺組織,MMP-9的錶達未見明顯差彆.KL-6介導的肺纖維化和FGF-10錶達異常導緻的肺-支氣管先天性髮育異常可能與肺大皰的形成相關,MMP-9介導的炎性反應與肺大皰形成的相關性尚待進一步研究.
목적 검측병비교세포인자폐포세포표면항원(KL)-6、성섬유세포생장인자(FGF)-10、기질금속단백매(MMP)-9재자발성기흉환자폐대포화포방정상폐조직중적표체,탐토폐대포형성과정중세포인자적의의.방법 24례자발성기흉환자,채용면역조직화학염색기술화매련면역흡부법(ELISA)검측폐대포조직급포방정상폐조직중적KL-6、FGF-10급MMP-9표체수평,배대t검험분석결과.결과 면역조화화ELISA결과균현시:폐대포조직중KL-6화FGF-10적표체명현고우포방정상폐조직(P＜0.05),단MMP-9재량조간차이무통계학의의(P＞0.05).결론 자발성기흉환자폐대포조직중KL-6화FGF-10적표체명현고우포방정상폐조직,MMP-9적표체미견명현차별.KL-6개도적폐섬유화화FGF-10표체이상도치적폐-지기관선천성발육이상가능여폐대포적형성상관,MMP-9개도적염성반응여폐대포형성적상관성상대진일보연구.
Objective Spontaneous pneumothorax occurred mainly because of bulla rupture and its formation process and pathogenesis were unknown,the study was to detect the express level of the cytokines KL-6,FGF-10 and MMP-9 in the spontaneous pneumothorax patients with bulla and researched its significance.Methods Selected 24 cases of bulla resection for spontaneous pneumothorax patients,the immunohistochemical staining techniques and enzyme-linked immunosorbent assay (ELISA) was taken to detect the expression level of KL-6,FGF-10 and MMP-9 of the bulla site and the bulla adjacent site.Results Immunohistochemical results showed that the staining intensity of the KL-6 and FGF-10 in groups of bulla site was higher than those in groups of bulla adjacent site while there was no significant difference of MMP-9 in the two groups.ELISA results showed that the expression levels of the KL-6 and FGF-10 in groups of bulla site are higher than those in groups of bulla adjacent site and the results had statistically significant (P ＜ 0.05),while there was no statistically significant of MMP-9 in the two groups(P ＞0.05).Conclusion The expression of the KL-6 and FGF-10 in the bulla site in primary spontaneous pneumothorax patients was higher than that in the normal site ; the pulmonary fibrosis mediated by KL-6 and the lung-bronchial congenital abnormalities mediated by abnormal expression of FGF-10 might have correlation with bulla formation.There was no statistically significant of the MMP-9 expression between the two groups and the correlation between inflammation mediated by MMP-9 and bulla formation was not clear.
