中华胸心血管外科杂志
中華胸心血管外科雜誌
중화흉심혈관외과잡지
Chinese Journal of Thoracic and Cardiovascular Surgery
2014年
7期
408-410,414
,共4页
罗文琦%甄文俊%欧阳小康%王怀斌%谭洁%马玉健
囉文琦%甄文俊%歐暘小康%王懷斌%譚潔%馬玉健
라문기%견문준%구양소강%왕부빈%담길%마옥건
冠状动脉旁路移植术%糖尿病%大隐静脉%内膜增生
冠狀動脈徬路移植術%糖尿病%大隱靜脈%內膜增生
관상동맥방로이식술%당뇨병%대은정맥%내막증생
Coronary artery bypass grafting%Diabetes mellitus%Great saphenous vein%Intimal hyperplasia
目的 研究合并糖尿病的冠状动脉粥样硬化性心脏病(CHD)患者行冠状动脉旁路移植术(CABG)前,大隐静脉(GSV)血管自身存在的内膜增生和核转录因子-kappa B(NF-κB)、血管内皮细胞黏附分子-1(VCAM-1)在血管内膜的表达情况.方法 从42例择期行CABG的患者中获取GSV,其中糖尿病患者20例,非糖尿病患者22例,对获取的GSV进行形态学分析,同时采用免疫组化染色方法测定血管内膜NF-κB、VCAM-1的表达.结果 糖尿病患者GSV内膜增生发生率(85.45％)显著高于非糖尿病患者(45.45％),且增生程度显著增强;糖尿病患者GSV内膜层中NF-κB、VCAM-1的表达显著高于非糖尿病患者,二者表达成正相关.结论 合并糖尿病的CHD患者行CABG前GSV存在显著内膜增生,且增生的内膜层中NF-κB、VCAM-1表达显著增高,可能与糖尿病患者CABG术后长期预后不良相关.
目的 研究閤併糖尿病的冠狀動脈粥樣硬化性心髒病(CHD)患者行冠狀動脈徬路移植術(CABG)前,大隱靜脈(GSV)血管自身存在的內膜增生和覈轉錄因子-kappa B(NF-κB)、血管內皮細胞黏附分子-1(VCAM-1)在血管內膜的錶達情況.方法 從42例擇期行CABG的患者中穫取GSV,其中糖尿病患者20例,非糖尿病患者22例,對穫取的GSV進行形態學分析,同時採用免疫組化染色方法測定血管內膜NF-κB、VCAM-1的錶達.結果 糖尿病患者GSV內膜增生髮生率(85.45％)顯著高于非糖尿病患者(45.45％),且增生程度顯著增彊;糖尿病患者GSV內膜層中NF-κB、VCAM-1的錶達顯著高于非糖尿病患者,二者錶達成正相關.結論 閤併糖尿病的CHD患者行CABG前GSV存在顯著內膜增生,且增生的內膜層中NF-κB、VCAM-1錶達顯著增高,可能與糖尿病患者CABG術後長期預後不良相關.
목적 연구합병당뇨병적관상동맥죽양경화성심장병(CHD)환자행관상동맥방로이식술(CABG)전,대은정맥(GSV)혈관자신존재적내막증생화핵전록인자-kappa B(NF-κB)、혈관내피세포점부분자-1(VCAM-1)재혈관내막적표체정황.방법 종42례택기행CABG적환자중획취GSV,기중당뇨병환자20례,비당뇨병환자22례,대획취적GSV진행형태학분석,동시채용면역조화염색방법측정혈관내막NF-κB、VCAM-1적표체.결과 당뇨병환자GSV내막증생발생솔(85.45％)현저고우비당뇨병환자(45.45％),차증생정도현저증강;당뇨병환자GSV내막층중NF-κB、VCAM-1적표체현저고우비당뇨병환자,이자표체성정상관.결론 합병당뇨병적CHD환자행CABG전GSV존재현저내막증생,차증생적내막층중NF-κB、VCAM-1표체현저증고,가능여당뇨병환자CABG술후장기예후불량상관.
Objective To study the pre-existing intimal hyperplasia and the expression of nuclear factor-κB (NF-κB) and vascular cell adhesion molecule 1 (VCAM-1) in great saphenous vein(GSV) among the patients with diabetes mellitus undergoing coronary artery bypass grafting(CABG).Methods Segments of GSV were collected from 20 patients with diabetes mellitus and 22 patients without diabetes mellitus undergoing CABG.Morphometric analysis was performed after hematoxylin-eosin (HE) staining and Masson staining by microscopic computer analysis.Immunohistochemistry staining was used to examine the expression of NF-κB and VCAM-1 in intima.Results Pre-existing intimal hyperplasia was more common and server in GSV of the patients with diabetes mellitus.The expression of NF-κB and VCAM-1 were significantly higher in intima of GSV in patients with diabetes mellitus than patients without diabetes mellitus.The expression of VCAM-1 was positively related to NFκB in intima of GSV.Conclusion The pre-existing intimal hyperplasia and the expression of NF-κB and VCAM-1 were more common and server in GSV of the patients with diabetes mellitus than patients without diabetes mellitus.This can partially explain the poor prognosis of patients with diabetes mellitus after CABG.
