中华血液学杂志
中華血液學雜誌
중화혈액학잡지
Chinese Journal of Hematology
2012年
11期
938-943
,共6页
许超平%蔡惠丽%何莉%马梓%刘尚勤
許超平%蔡惠麗%何莉%馬梓%劉尚勤
허초평%채혜려%하리%마재%류상근
多发性骨髓瘤%黄芩素%细胞增殖%细胞迁移%β-catenin
多髮性骨髓瘤%黃芩素%細胞增殖%細胞遷移%β-catenin
다발성골수류%황금소%세포증식%세포천이%β-catenin
Multiple myeloma%Baicalein%Cell proliferation%Cell migration%β-catenin
目的 探讨黄芩素对多发性骨髓瘤(MM)细胞增殖和迁移能力的影响及分子机制.方法 以不同浓度黄芩素处理MM细胞株RPMI 8226和U266细胞不同时间后,应用四甲基偶氮唑盐(MTT)比色实验检测黄芩素对MM细胞增殖的影响；用黄芩素和(或)IL-6处理RPMI 8226、U266细胞,激光共聚焦及Western blot检测处理前后细胞中β连环素(β-catenin)蛋白表达；Transwell小室实验检测不同浓度黄芩素处理前后RPMI 8226和U266细胞的迁移能力；RT-PCR检测不同浓度黄芩素处理前后细胞内β-catenin、c-myc、细胞周期素D1(Cyclin D1)和细胞迁移相关基因整联蛋白β7(integrin β7)基因mRNA表达.结果 黄芩素呈浓度和时间依赖性抑制RPMI 8226和U266细胞的增殖；激光共聚焦及Westem blot结果显示黄芩素能够抑制β-catenin的表达从而抵抗IL-6对MM细胞的促增殖作用；RT-PCR检测显示黄芩素能够抑制β-catenin、c-myc、cyclin D1和integrin β7的mRNA表达；Transwell小室迁移实验表明,在基质细胞衍生因子(SDF-1)的趋化下,黄芩素以浓度依赖的方式降低MM细胞的迁移能力.结论 黄芩素具有抑制MM细胞增殖和迁移的作用,其分子机制与抑制增殖相关基因β-catenin、c-myc、cyclin D1和integrin β7的表达有关.
目的 探討黃芩素對多髮性骨髓瘤(MM)細胞增殖和遷移能力的影響及分子機製.方法 以不同濃度黃芩素處理MM細胞株RPMI 8226和U266細胞不同時間後,應用四甲基偶氮唑鹽(MTT)比色實驗檢測黃芩素對MM細胞增殖的影響；用黃芩素和(或)IL-6處理RPMI 8226、U266細胞,激光共聚焦及Western blot檢測處理前後細胞中β連環素(β-catenin)蛋白錶達；Transwell小室實驗檢測不同濃度黃芩素處理前後RPMI 8226和U266細胞的遷移能力；RT-PCR檢測不同濃度黃芩素處理前後細胞內β-catenin、c-myc、細胞週期素D1(Cyclin D1)和細胞遷移相關基因整聯蛋白β7(integrin β7)基因mRNA錶達.結果 黃芩素呈濃度和時間依賴性抑製RPMI 8226和U266細胞的增殖；激光共聚焦及Westem blot結果顯示黃芩素能夠抑製β-catenin的錶達從而牴抗IL-6對MM細胞的促增殖作用；RT-PCR檢測顯示黃芩素能夠抑製β-catenin、c-myc、cyclin D1和integrin β7的mRNA錶達；Transwell小室遷移實驗錶明,在基質細胞衍生因子(SDF-1)的趨化下,黃芩素以濃度依賴的方式降低MM細胞的遷移能力.結論 黃芩素具有抑製MM細胞增殖和遷移的作用,其分子機製與抑製增殖相關基因β-catenin、c-myc、cyclin D1和integrin β7的錶達有關.
목적 탐토황금소대다발성골수류(MM)세포증식화천이능력적영향급분자궤제.방법 이불동농도황금소처리MM세포주RPMI 8226화U266세포불동시간후,응용사갑기우담서염(MTT)비색실험검측황금소대MM세포증식적영향；용황금소화(혹)IL-6처리RPMI 8226、U266세포,격광공취초급Western blot검측처리전후세포중β련배소(β-catenin)단백표체；Transwell소실실험검측불동농도황금소처리전후RPMI 8226화U266세포적천이능력；RT-PCR검측불동농도황금소처리전후세포내β-catenin、c-myc、세포주기소D1(Cyclin D1)화세포천이상관기인정련단백β7(integrin β7)기인mRNA표체.결과 황금소정농도화시간의뢰성억제RPMI 8226화U266세포적증식；격광공취초급Westem blot결과현시황금소능구억제β-catenin적표체종이저항IL-6대MM세포적촉증식작용；RT-PCR검측현시황금소능구억제β-catenin、c-myc、cyclin D1화integrin β7적mRNA표체；Transwell소실천이실험표명,재기질세포연생인자(SDF-1)적추화하,황금소이농도의뢰적방식강저MM세포적천이능력.결론 황금소구유억제MM세포증식화천이적작용,기분자궤제여억제증식상관기인β-catenin、c-myc、cyclin D1화integrin β7적표체유관.
Objective To investigate the effect of baicalein on proliferation and migration of multiple myeloma(MM) cell lines and its molecular mechanism.Methods The MM cell line RPMI-8226 and U266 cells were used as the model,and treated with different concentration and time of baicalein the effect of baicalein on the MM cells proliferation was assessed by MTT assay.With or without baicalein or Interleukin-6 (IL-6) treatment,the β-catenin protein level was analyzed by immunofluorescence assay and western blot assay and mRNA levels of β-catenin,c-myc,cyclin D1 and integrin 7 gene by RT-PCR.Transwell chamber migration assay was used to detect the cells migration ability with different concentration of baicalein cultured.Results Baicalein inhibited the MM cell line RPMI 8226 and U266 cell proliferation in a dose-and time-dependent manner.It simultaneously inhibited β-catenin protein level to resist the effect of IL-6 on inducing MM cell proliferation,and resulted in decrease of β-catenin,c-myc,cyclinD1 and integrin β7 mRNA levels.Baicalein also decreased migration ability of MM cells in a dose-dependent manner by SDF-1.Conclusion Baicalein can inhibit MM cells proliferation and migration,and its molecular mechanisms are associated with inhibition of proliferation related genes β-catenin,c-myc,cyclinD1 and integrin β7 expression.
