CAJ | 학술논문

目的探讨趋化因子受体CXCR3和CCR5在原发免疫性血小板减少症(ITP)患者脾脏中的表达及其临床意义.方法以10例ITP患者(ITP组)为研究对象,并以8例创伤性脾破裂患者脾切除标本作为正常对照.应用免疫组化法检测脾脏组织CXCR3、CCR5阳性表达率,用Western blot 法检测脾脏组织中CXCR3和CCR5的蛋白表达水平,逆转录聚合酶链反应检测CXCR3和CCR5mRNA表达水平.结果免疫组化结果显示ITP患者脾脏组织CXCR3、CCR5阳性表达率(90％、100％)均高于对照组(75％、87.5％),差异均有统计学意义(P＜0.05)；ITP患者脾脏组织CXCR3蛋白、mRNA表达量分别为对照组的3.0倍、3.5倍；CCR5蛋白、mRNA表达量分别为对照组的1.2倍、1.7倍,差异均有统计学意义(P＜0.05).结论 CXCR3及CCR5的高表达可能在ITP患者脾脏免疫紊乱中起着一定的作用.
목적 탐토추화인자수체CXCR3화CCR5재원발면역성혈소판감소증(ITP)환자비장중적표체급기림상의의.방법 이10례ITP환자(ITP조)위연구대상,병이8례창상성비파렬환자비절제표본작위정상대조.응용면역조화법검측비장조직CXCR3、CCR5양성표체솔,용Western blot 법검측비장조직중CXCR3화CCR5적단백표체수평,역전록취합매련반응검측CXCR3화CCR5mRNA표체수평.결과 면역조화결과현시ITP환자비장조직CXCR3、CCR5양성표체솔(90％、100％)균고우대조조(75％、87.5％),차이균유통계학의의(P＜0.05)；ITP환자비장조직CXCR3단백、mRNA표체량분별위대조조적3.0배、3.5배；CCR5단백、mRNA표체량분별위대조조적1.2배、1.7배,차이균유통계학의의(P＜0.05).결론 CXCR3급CCR5적고표체가능재ITP환자비장면역문란중기착일정적작용.
Objective To study CXCR3 and CCR5 chemokine receptor expression in spleens of patients with primary immune thrombocytopenia (ITP) and its clinical significance.Methods The splenectomy specimens from 10 ITP patients (ITP group) and 8 patients with traumatic splenic rupture (normal control group) were studied.Immunohistochemistry (IHC) was used to study the positive rate of CXCR3 and CCR5.Western blot was performed to detect CXCR3 and CCR5 protein expression,while real-time polymerase chain reaction (RT-PCR) was conducted to analyze their mRNA expression.Results The positive rate of CXCR3 and CCR5 were both higher in ITP group (90％ and 100％,respectively) than those in control group (75％and 87.5 ％,respectively) (P ＜ 0.05).The differences were statistically significant (P ＜ 0.05).Protein and mRNA level of CXCR3 in ITP group were 3.0 and 3.5 times as high as those in control group,respectively.Those of CCR5 in ITP group were 1.2 and 1.7 times as high as those in control group,respectively.Conclusion High expression of CXCR3 and CCR5 may play a part in the splenic immune disorders in patients with ITP.