中华血液学杂志
中華血液學雜誌
중화혈액학잡지
Chinese Journal of Hematology
2012年
12期
1015-1019
,共5页
王欣如%李丽珍%黄涛%李颢%王鲁群%李湘新%李芳邻
王訢如%李麗珍%黃濤%李顥%王魯群%李湘新%李芳鄰
왕흔여%리려진%황도%리호%왕로군%리상신%리방린
淋巴瘤,霍奇金%多态性,单核苷酸%转化生长因子β1%白细胞介素4%受体
淋巴瘤,霍奇金%多態性,單覈苷痠%轉化生長因子β1%白細胞介素4%受體
림파류,곽기금%다태성,단핵감산%전화생장인자β1%백세포개소4%수체
Lymphoma,Hodgkin%Polymorphism,single nucleotide%Transforming growth factor β1%Receptor,interleukin-4
目的 研究转化生长因子-β1(TGF-β1)基因G-800A、C-509T及IL-4受体(IL-4R)基因V75I位点单核苷酸多态性与成人经典霍奇金淋巴瘤(CHL)易感性的关系.方法 应用PCR-限制性片段长度多态性技术研究TGF-β1 G-800A、C-509T,IL-4R V75I位点多态性分布特点,并结合临床资料分析其与CHL发病风险的关系.结果 TGF-β1 C-509T、G-800A存在明显连锁不均衡性(D′=0.879,r2=0.83,P=0.020);混合细胞型CHL组与对照组GT单倍体型的分布频数分别为53.1%和34.2%,差异有统计学意义(OR=2.35,P=0.000);突变型基因T/T在疾病组和对照组分布频数分别为38.8%和15.3%,差异有统计学意义(OR=3.654,P=0.000);结节硬化型CHL 患者IL-4R V75I位点突变型基因A/A在疾病组和对照组分布频数分别为19.2%和41.75%,差异有统计学意义(OR=3.156,P=0.000).结论 TGF-β1 G-800A、C-509T连锁单核苷酸多态性位点、IL-4R V75I位点多态性与中国人群CHL的发病风险存在相关性.
目的 研究轉化生長因子-β1(TGF-β1)基因G-800A、C-509T及IL-4受體(IL-4R)基因V75I位點單覈苷痠多態性與成人經典霍奇金淋巴瘤(CHL)易感性的關繫.方法 應用PCR-限製性片段長度多態性技術研究TGF-β1 G-800A、C-509T,IL-4R V75I位點多態性分佈特點,併結閤臨床資料分析其與CHL髮病風險的關繫.結果 TGF-β1 C-509T、G-800A存在明顯連鎖不均衡性(D′=0.879,r2=0.83,P=0.020);混閤細胞型CHL組與對照組GT單倍體型的分佈頻數分彆為53.1%和34.2%,差異有統計學意義(OR=2.35,P=0.000);突變型基因T/T在疾病組和對照組分佈頻數分彆為38.8%和15.3%,差異有統計學意義(OR=3.654,P=0.000);結節硬化型CHL 患者IL-4R V75I位點突變型基因A/A在疾病組和對照組分佈頻數分彆為19.2%和41.75%,差異有統計學意義(OR=3.156,P=0.000).結論 TGF-β1 G-800A、C-509T連鎖單覈苷痠多態性位點、IL-4R V75I位點多態性與中國人群CHL的髮病風險存在相關性.
목적 연구전화생장인자-β1(TGF-β1)기인G-800A、C-509T급IL-4수체(IL-4R)기인V75I위점단핵감산다태성여성인경전곽기금림파류(CHL)역감성적관계.방법 응용PCR-한제성편단장도다태성기술연구TGF-β1 G-800A、C-509T,IL-4R V75I위점다태성분포특점,병결합림상자료분석기여CHL발병풍험적관계.결과 TGF-β1 C-509T、G-800A존재명현련쇄불균형성(D′=0.879,r2=0.83,P=0.020);혼합세포형CHL조여대조조GT단배체형적분포빈수분별위53.1%화34.2%,차이유통계학의의(OR=2.35,P=0.000);돌변형기인T/T재질병조화대조조분포빈수분별위38.8%화15.3%,차이유통계학의의(OR=3.654,P=0.000);결절경화형CHL 환자IL-4R V75I위점돌변형기인A/A재질병조화대조조분포빈수분별위19.2%화41.75%,차이유통계학의의(OR=3.156,P=0.000).결론 TGF-β1 G-800A、C-509T련쇄단핵감산다태성위점、IL-4R V75I위점다태성여중국인군CHL적발병풍험존재상관성.
Objective This study was aimed to analyze the relationship between single nucleotide polymorphisms of transforming growth factor-β1 G-800A and C-509T, interleukin-4 receptor V75I and susceptibility of CHL in adults. Methods Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was applied to analyze the expressed alleles of the selected SNP loca. The relationship between genomic polymorphisms of TGF-β1 and IL-4R and susceptibility of CHL were coupled with clinical data. Results TGF-β1G-800A and TGF-β1C-509T had obvious linkage equilibrium（D′=0.879，r2=0.83，P=0.020）. GT haplotype distribution frequencies in mixed cellularity Hodgkin lymphoma cases and control group were of 53.1% and 34.2%, respectively, with statistically significant (OR=2.35, P=0.000); distribution frequencies of mutant gene T/T in disease and control groups were of 38.8% and 15.3%, respectively, also with statistically significant (OR=3.654, P=0.000); frequencies of nodular sclerosis CHL patients with IL-4R V75I mutant gene A/A in disease and control groups were of 19.2% and 41.75%, respectively, also with statistically significant (OR=3.156, P=0.000). Conclusion Single nucleotide polymorphisms of TGF-β1 G-800A, C-509T and IL-4R V75I has a significant correlation with Chinese susceptibility to classical Hodgkin lymphoma.
