CAJ | 학술논문

目的探讨干扰素-γ(IFN-γ)对正常人骨髓间充质干细胞(MSC)免疫活性的影响,阐明IFN-γ与MSC在免疫抑制中的协同作用.方法 ①ELISA法检测IFN-γ(终浓度100 ng/ml)对正常人骨髓来源MSC前列腺素E2(PGE2)、肝细胞生长因子(HGF)和转化生长因子β1(TGF-β1)表达水平的影响.②RT-PCR法检测100 ng/ml IFN-γ作用下MSC内吲哚胺2,3-双加氧酶(IDO)基因表达水平变化.③体外刺激正常人外周血T细胞增殖,并与正常人骨髓来源MSC共培养,检测T细胞增殖水平;在共培养体系中分别加入重组人IFN-γ(100 ng/ml)及鼠抗人IFN-γ单克隆抗体(单抗,5μg/ml),检测T细胞增殖水平变化.结果 ①MSC单独培养24 ～ 48 h可检测到PGE2、HGF、TGF-β1三种细胞因子表达;IFN-γ可促进MSC上述细胞因子的表达[实验组及对照组PGE2、HGF、TGF-β1表达水平分别为(1715.5 ±628.6) pg/ml对(1344.5±709.4) pg/ml;(4031.8±1496.8) pg/ml对(2452.4±1375.3)pg/ml;(1753.5±413.8) pg/ml对(1026.6±450.5) pg/ml,P值分别为0.001、0.011及＜0.001].②MSC单独培养48 h后,RT-PCR法未检测到IDO基因表达.与IFN-γ共培养后,IDO基因呈高水平表达.③在体外MSC可以显著抑制T细胞增殖,外源性IFN-γ不影响MSC对T细胞增殖的抑制作用[T细胞增殖抑制率分别为(40.4±10.9)％和(36.7±7.4)％,P=0.272];在反应体系中加入抗IFN-γ单抗后,MSC抑制T细胞增殖作用显著减弱[T细胞增殖抑制率分别为(40.4±10.9)％和(23.9±7.6)％,P =0.002].结论 MSC组成性表达PGE2、HGF及TGF-β1等免疫抑制性细胞因子,在一定水平IFN-γ作用下,MSC分泌上述3种细胞因子水平明显上调,IDO mRNA表达水平显著增高,MSC免疫活性增强.骨髓MSC在体外可以显著抑制T淋巴细胞增殖,IFN-γ与MSC在免疫抑制中具有协同效应.
목적 탐토간우소-γ(IFN-γ)대정상인골수간충질간세포(MSC)면역활성적영향,천명IFN-γ여MSC재면역억제중적협동작용.방법 ①ELISA법검측IFN-γ(종농도100 ng/ml)대정상인골수래원MSC전렬선소E2(PGE2)、간세포생장인자(HGF)화전화생장인자β1(TGF-β1)표체수평적영향.②RT-PCR법검측100 ng/ml IFN-γ작용하MSC내신타알2,3-쌍가양매(IDO)기인표체수평변화.③체외자격정상인외주혈T세포증식,병여정상인골수래원MSC공배양,검측T세포증식수평;재공배양체계중분별가입중조인IFN-γ(100 ng/ml)급서항인IFN-γ단극륭항체(단항,5μg/ml),검측T세포증식수평변화.결과 ①MSC단독배양24 ～ 48 h가검측도PGE2、HGF、TGF-β1삼충세포인자표체;IFN-γ가촉진MSC상술세포인자적표체[실험조급대조조PGE2、HGF、TGF-β1표체수평분별위(1715.5 ±628.6) pg/ml대(1344.5±709.4) pg/ml;(4031.8±1496.8) pg/ml대(2452.4±1375.3)pg/ml;(1753.5±413.8) pg/ml대(1026.6±450.5) pg/ml,P치분별위0.001、0.011급＜0.001].②MSC단독배양48 h후,RT-PCR법미검측도IDO기인표체.여IFN-γ공배양후,IDO기인정고수평표체.③재체외MSC가이현저억제T세포증식,외원성IFN-γ불영향MSC대T세포증식적억제작용[T세포증식억제솔분별위(40.4±10.9)％화(36.7±7.4)％,P=0.272];재반응체계중가입항IFN-γ단항후,MSC억제T세포증식작용현저감약[T세포증식억제솔분별위(40.4±10.9)％화(23.9±7.6)％,P =0.002].결론 MSC조성성표체PGE2、HGF급TGF-β1등면역억제성세포인자,재일정수평IFN-γ작용하,MSC분비상술3충세포인자수평명현상조,IDO mRNA표체수평현저증고,MSC면역활성증강.골수MSC재체외가이현저억제T림파세포증식,IFN-γ여MSC재면역억제중구유협동효응.
Objective To investigate mesenchymal stem cells (MSCs) immunosuppressive activity in the presence of interferon-gamma (IFN-γ) to reveal synergistic immunomodulatory effects of IFN-γ and MSCs.Methods ① MSCs were cultured in the presence or absence of IFN-γ(100 ng/ml),the supernatants were collected for measurements of PGE2 、HGF and TGF-β1 by ELISA kits.② MSCs were cultured in the presence or absence of IFN-γ (100 ng/ml) for 48 h.The cDNA was analysed for the expression of human indoleamine 2,3-dioxygenase (IDO)mRNA by semiquantitative RT-PCR.③ Mononuclear cells (MNCs)were extracted from peripheral blood of healthy donors.The T cell proliferation was tested in the co-culture system added with MSCs,recombinant human IFN-γ (100 ng/ml) and anti-IFN-γmAb (5 μg/ml) by BrdU ELISA kit.Results ①The immunosuppressive cytokines PGE2 、HGF and TGF-β1 were detectable within 24-48 h in the supernatants.Their expressions were significantly up-regulated in the presence of IFN-γ.Concentrations of these cytokines were as of (1715.5 ±628.6) pg/ml vs (1344.5 ± 709.4) pg/ml (P =0.001) ; (4031.8 ± 1496.8) pg/ml vs (2452.4 ± 1375.3) pg/ml (P =0.011) ; (1753.5 ± 413.8) pg/ml vs (1026.6 ± 450.5) pg/ml (P ＜ 0.001),respectively.②The expression of IDO mRNA was undetectable when MSCs were cultured alone.In contrast,The IDO mRNA expression was remarkably enhanced in the presence of IFN-γ.③ Bone marrow-derived MSCs remarkably suppressed allogeneic T cell proliferation in vitro.Addition of exogenous IFN-γ had no significant effect on the inhibitory capacity of MSCs,the inhibitory ratios of T cell proliferation were (40.4 ± 10.9) ％ vs (36.7 ± 7.4) ％ (P =0.272).By contrast,the inhibitory ratio of T cell proliferation was significantly decreased in the presence of anti-IFN-γ mAb [(40.4 ±10.9) ％ vs (23.9 ± 7.6) ％,P =0.002].Conclusions ① Human MSCs constitutively expressed immunosuppressive concentrations of PGE2,HGF and TGF-β1,and their expressions were significantly up-regulated by IFN-γ.②IFN-γ-induced expression of IDO on MSCs involved in tryptophan catabolism.③MSCs notably suppressed allogeneic T cell proliferation in vitro.IFN-γpromoted the immunosuppressive capacity of human MSCs,indicating the synergistic immunomodulatory effect of IFN-γ and MSCs.