CAJ | 학술논문

目的应用差异蛋白质谱的方法研究急性白血病患者与健康人血清中蛋白质谱的表达差异.方法提取51例急性白血病患者和10名健康志愿者的外周血血清,去除血清样品中的高丰度蛋白,用双向凝胶电泳方法进行血清低丰度蛋白质的分离,找出急性白血病患者与健康人血清中的差异蛋白点,切出差异蛋白点后进行脱色、胶内酶切、肽段提取,表面增强激光解吸-电离飞行时间质谱(MALDI TOF/TOF MS)进行分析获得肽质量指纹图.使用蛋白质数据库MSDB提供的Masort检索程序进行评价.结果成组t检验结果显示,与健康对照组比较,急性白血病患者血清有19个差异有统计学意义的异常表达蛋白点(P＜0.05),经过质谱鉴定α1-胰蛋白酶抑制剂(P＜0.01)、前白蛋白(P＜0.01)、胰蛋白酶抑制剂(P＜0.01)、载脂蛋白E(P＜0.01)、载脂蛋白A-Ⅳ(P＜0.01)为表达下降,而视黄醇结合蛋白(P＜0.05)、结合珠蛋白HP2(P ＜0.05)、血清外源性凝集素(P＜0.05)、H因子同源物(P＜0.05)、血清淀粉样蛋白A1(P＜0.01)为表达上调,进一步分层分析发现血清外源性凝集素表达高的急性白血病患者治疗效果较差.结论急性白血病患者外周血血清中存在多种蛋白质的差异表达,血清外源性凝集素的表达差异与治疗效果相关,这些蛋白的差异表达可作为新的急性白血病诊断标志或者预后指标.
목적 응용차이단백질보적방법연구급성백혈병환자여건강인혈청중단백질보적표체차이.방법 제취51례급성백혈병환자화10명건강지원자적외주혈혈청,거제혈청양품중적고봉도단백,용쌍향응효전영방법진행혈청저봉도단백질적분리,조출급성백혈병환자여건강인혈청중적차이단백점,절출차이단백점후진행탈색、효내매절、태단제취,표면증강격광해흡-전리비행시간질보(MALDI TOF/TOF MS)진행분석획득태질량지문도.사용단백질수거고MSDB제공적Masort검색정서진행평개.결과 성조t검험결과현시,여건강대조조비교,급성백혈병환자혈청유19개차이유통계학의의적이상표체단백점(P＜0.05),경과질보감정α1-이단백매억제제(P＜0.01)、전백단백(P＜0.01)、이단백매억제제(P＜0.01)、재지단백E(P＜0.01)、재지단백A-Ⅳ(P＜0.01)위표체하강,이시황순결합단백(P＜0.05)、결합주단백HP2(P ＜0.05)、혈청외원성응집소(P＜0.05)、H인자동원물(P＜0.05)、혈청정분양단백A1(P＜0.01)위표체상조,진일보분층분석발현혈청외원성응집소표체고적급성백혈병환자치료효과교차.결론 급성백혈병환자외주혈혈청중존재다충단백질적차이표체,혈청외원성응집소적표체차이여치료효과상관,저사단백적차이표체가작위신적급성백혈병진단표지혹자예후지표.
Objective To study the serum protein differential expression in acute leukemia patients and healthy control by differential protein mass spectrometry.Methods Serum proteins of 51 acute leukemia (AL) patients and 10 healthy donors were extracted from their peripheral blood.After removing high abundance protein,serum low abundance proteins were separated by two dimensional gel electrophoresis,the differences of serum proteins in AL patients and healthy human were identified.The protein spots with differential expression were cut out and then undergone bleaching,gel digestion and peptide extraction.The peptide mass fingerprint analysis was performed by using MALDI TOF/TOF MS.The protein database MSDB Masort retrieval program was used to evaluate the results.Results Using Student' s t test,19 statistically significant abnormal expression proteins in the serum of AL patients were found compared with the healthy controls (P ＜ 0.05).The expression of α1-trypsin inhibitor (P ＜ 0.01),prealbumin (P ＜ 0.01),trypsin inhibitor (P ＜ 0.01),apolipoprotein E (P ＜ 0.01) and apolipoprotein A-Ⅳ (P ＜ 0.01) decreased,while retinol binding protein (P ＜ 0.05),globin HP2 (P ＜ 0.05),serum lectin (P ＜ 0.05),H factor homologue protein (P ＜ 0.05) and serum amyloid A1 (P ＜ 0.01) increased.Further stratified analysis found that high serum lectin expression in AL patient resulted in poor outcomes.Conclusion There are a variety of serum proteins with differential expression in peripheral blood of AL patients.The differential expression of serum lectin is related to the therapeutic effect.The differential expression of these proteins can be used as a new diagnosis marker or prognostic indicator for acute leukemia.