中华血液学杂志
中華血液學雜誌
중화혈액학잡지
Chinese Journal of Hematology
2013年
8期
679-684
,共6页
王洪涛%赵翔宇%赵晓甦%韩婷婷%吕萌%常英军%黄晓军
王洪濤%趙翔宇%趙曉甦%韓婷婷%呂萌%常英軍%黃曉軍
왕홍도%조상우%조효소%한정정%려맹%상영군%황효군
造血干细胞移植%T淋巴细胞,调节性%Th1细胞%Th17细胞%复发%移植物抗宿主病
造血榦細胞移植%T淋巴細胞,調節性%Th1細胞%Th17細胞%複髮%移植物抗宿主病
조혈간세포이식%T림파세포,조절성%Th1세포%Th17세포%복발%이식물항숙주병
Hematopoietic stem cell transplantation%Regulatory T cells%Th1 cells%Th17cells%Recurrence%Graft versus host disease
目的 探讨异基因造血干细胞移植(allo-HSCT)后患者调节性T细胞、效应性T细胞以及两者之间的比例与疾病复发及慢性移植物抗宿主病(cGVHD)的关系.方法 30例恶性血液病患者按复发与cGVHD的发生情况分成4组,分别为有复发有cGVHD组(4例)、有复发无cGVHD组(5例)、无复发有cGVHD组(14例)、无复发无cGVHD组(7例) 应用流式细胞术检测患者骨髓中CD4+CD25-CD69+调节T细胞和外周血中CD4+ CD25+ToxP3+调节T细胞、Th1细胞和Th17细胞占CD4-T细胞比例,比较不同组间调节性T细胞、效应性T细胞以及两者比例.结果 CD4+CD25ToxP3+调节T细胞、CD4+CD25 CD69+调节T细胞、Th1细胞与Th17细胞比例在不同组间差异均无统计学意义(P值均＞0.05).9例复发患者的CD4+CD25-CD69+调节T细胞/Th1细胞比值为0.211±0.117,21例无复发者为0.133±0.160,差异有统计学意义(P=0.033),12例无cGVHD的患者中复发组亦高于无复发组(0.167±0.073对0.073±0.057,P=0.048),有cGVHD同时伴有复发者高于无cGVHD无复发者(0.218±0.113对0.073±0.057,P=0.024).18例发生cGVHD的患者中CD4+CD25+FoxP3+调节T细胞/Th17细胞比值为1.975±2.045,12例无cGVHD者为3.198±1.132,差异有统计学意义(P=0.010),21例无复发患者中有cGVHD者低于无cGVHD者(1.695±1.178对3.446± 1.376,P=0.028).结论 CD4+CD25-CD69+调节T细胞/Th1细胞比值在复发时升高,CD4+CD25ToxP3+调节T细胞/Th 17细胞比值在cGVHD发生时降低,提示调节性T细胞与效应性T细胞的比例与复发及cGVHD相关.
目的 探討異基因造血榦細胞移植(allo-HSCT)後患者調節性T細胞、效應性T細胞以及兩者之間的比例與疾病複髮及慢性移植物抗宿主病(cGVHD)的關繫.方法 30例噁性血液病患者按複髮與cGVHD的髮生情況分成4組,分彆為有複髮有cGVHD組(4例)、有複髮無cGVHD組(5例)、無複髮有cGVHD組(14例)、無複髮無cGVHD組(7例) 應用流式細胞術檢測患者骨髓中CD4+CD25-CD69+調節T細胞和外週血中CD4+ CD25+ToxP3+調節T細胞、Th1細胞和Th17細胞佔CD4-T細胞比例,比較不同組間調節性T細胞、效應性T細胞以及兩者比例.結果 CD4+CD25ToxP3+調節T細胞、CD4+CD25 CD69+調節T細胞、Th1細胞與Th17細胞比例在不同組間差異均無統計學意義(P值均＞0.05).9例複髮患者的CD4+CD25-CD69+調節T細胞/Th1細胞比值為0.211±0.117,21例無複髮者為0.133±0.160,差異有統計學意義(P=0.033),12例無cGVHD的患者中複髮組亦高于無複髮組(0.167±0.073對0.073±0.057,P=0.048),有cGVHD同時伴有複髮者高于無cGVHD無複髮者(0.218±0.113對0.073±0.057,P=0.024).18例髮生cGVHD的患者中CD4+CD25+FoxP3+調節T細胞/Th17細胞比值為1.975±2.045,12例無cGVHD者為3.198±1.132,差異有統計學意義(P=0.010),21例無複髮患者中有cGVHD者低于無cGVHD者(1.695±1.178對3.446± 1.376,P=0.028).結論 CD4+CD25-CD69+調節T細胞/Th1細胞比值在複髮時升高,CD4+CD25ToxP3+調節T細胞/Th 17細胞比值在cGVHD髮生時降低,提示調節性T細胞與效應性T細胞的比例與複髮及cGVHD相關.
목적 탐토이기인조혈간세포이식(allo-HSCT)후환자조절성T세포、효응성T세포이급량자지간적비례여질병복발급만성이식물항숙주병(cGVHD)적관계.방법 30례악성혈액병환자안복발여cGVHD적발생정황분성4조,분별위유복발유cGVHD조(4례)、유복발무cGVHD조(5례)、무복발유cGVHD조(14례)、무복발무cGVHD조(7례) 응용류식세포술검측환자골수중CD4+CD25-CD69+조절T세포화외주혈중CD4+ CD25+ToxP3+조절T세포、Th1세포화Th17세포점CD4-T세포비례,비교불동조간조절성T세포、효응성T세포이급량자비례.결과 CD4+CD25ToxP3+조절T세포、CD4+CD25 CD69+조절T세포、Th1세포여Th17세포비례재불동조간차이균무통계학의의(P치균＞0.05).9례복발환자적CD4+CD25-CD69+조절T세포/Th1세포비치위0.211±0.117,21례무복발자위0.133±0.160,차이유통계학의의(P=0.033),12례무cGVHD적환자중복발조역고우무복발조(0.167±0.073대0.073±0.057,P=0.048),유cGVHD동시반유복발자고우무cGVHD무복발자(0.218±0.113대0.073±0.057,P=0.024).18례발생cGVHD적환자중CD4+CD25+FoxP3+조절T세포/Th17세포비치위1.975±2.045,12례무cGVHD자위3.198±1.132,차이유통계학의의(P=0.010),21례무복발환자중유cGVHD자저우무cGVHD자(1.695±1.178대3.446± 1.376,P=0.028).결론 CD4+CD25-CD69+조절T세포/Th1세포비치재복발시승고,CD4+CD25ToxP3+조절T세포/Th 17세포비치재cGVHD발생시강저,제시조절성T세포여효응성T세포적비례여복발급cGVHD상관.
Objective To investigate the association of the ratio of regulatory and effector T cells with recurrence and chronic graft-versus-host disease (cGVHD) after allogeneic hematopoietic stem cell transplantation (allo-HSCT).Methods Thirty patients with hematological malignancies who underwent allo-HSCT were classified as recurrence with cGVHD (n=4),non-recurrence with cGVHD (n=14),recurrence without cGVHD (n=5) and non-recurrence without cGVHD (n=7).The different percentage of CD4-CD25-CD69+ regulatory T cells in bone marrow and CD4-CD25+FoxP3 regulatory T cells,Th1 cells and Th17 cells in peripheral blood were analyzed by flow cytometry.Results There were no significant differences in all these T-cell subsets among different groups (P ＞ 0.05).While the ratio of CD4+CD25 CD69+ regulatory T cells and Thl cells (0.211 ±0.177) in 9 recurrence patients was significant higher than that (0.133 ± 0.160) in 21 non-recurrence patients (P=0.033).The ratio were also significance between recurrence without cGVHD and non-recurrence without cGVHD patients (0.167±0.073 vs 0.073±0.057,P=0.048),and between recurrence with cGVHD and non-recurrence without cGVHD patients (0.218±0.113vs 0.073±0.057,P=0.024).Furthermore,the ratio of CD4+CD25 +FoxP3+ regulatory T cells and Th17 cells was significant lower (1.975 ±2.045) in 18 cGVHD patients than that of 12 without cGVHD patients (3.198±1.132,P=0.010),and the ratio was also significant lower in non-recurrence patients with cGVHD (1.695±1.178) than that of without cGVHD (3.446±1.376,P=0.028).Conclusion Our results show that the ratio of CD4+CD25 CD69+ regulatory T cells and Th1 cells raise in recurrence patients,and the ratio of CD4+CD25+FoxP3+ regulatory T cells and Th 17 decrease in cGVHD patients,which suggest that the ratio of regulatory and effector T cells had association with recurrence and cGVHD in patients with allo-HSCT.
