CAJ | 학술논문

目的探讨间变性大细胞淋巴瘤(ALCL)患者骨髓及外周血NPM-ALK融合基因的表达与预后的关系.方法应用RT-PCR法检测21例ALCL患者骨髓(21份标本)及外周血(15份标本)细胞NPM-ALK融合基因的表达,并对其与患者的预后及临床特征之间的关系进行统计学分析.结果 21例患者中男12例,女9例,中位年龄9(2～14)岁.21例患者中位随访时间31个月.骨髓细胞NPM-ALK阳性患者3年无事件生存率为(35.6±18.6)％,阴性患者为(91.7士8.0)％,差异有统计学意义(P=0.038).患者骨髓细胞NPM-ALK阳性与其有3个以上器官受累(P=0.032)有相关性.86.7％的患者外周血与骨髓NPM-ALK检测结果一致.结论通过RT-PCR法检测ALCL患者骨髓及外周血NPM-ALK融合基因表达,可以证实患者血循环中有无微小肿瘤细胞播散,阳性结果是重要的预后不良因素,对指导分层治疗有重要的临床意义.
목적 탐토간변성대세포림파류(ALCL)환자골수급외주혈NPM-ALK융합기인적표체여예후적관계.방법 응용RT-PCR법검측21례ALCL환자골수(21빈표본)급외주혈(15빈표본)세포NPM-ALK융합기인적표체,병대기여환자적예후급림상특정지간적관계진행통계학분석.결과 21례환자중남12례,녀9례,중위년령9(2～14)세.21례환자중위수방시간31개월.골수세포NPM-ALK양성환자3년무사건생존솔위(35.6±18.6)％,음성환자위(91.7사8.0)％,차이유통계학의의(P=0.038).환자골수세포NPM-ALK양성여기유3개이상기관수루(P=0.032)유상관성.86.7％적환자외주혈여골수NPM-ALK검측결과일치.결론 통과RT-PCR법검측ALCL환자골수급외주혈NPM-ALK융합기인표체,가이증실환자혈순배중유무미소종류세포파산,양성결과시중요적예후불량인소,대지도분층치료유중요적림상의의.
Objective To investigate the expression of NPM-ALK fusion gene in bone marrow (BM) and peripheral blood (PB) in anaplastic large cell lymphoma (ALCL) patients and its prognostic significance.Methods NPM-ALK fusion gene of 21 BM and 15 PB samples from patients with NPMALK positive ALCL was detected by RT-PCR,and the relationship between NPM-ALK expression and prognosis and clinical characters was evaluated.Results Of the 21 patients,12 cases were male and 9 case were female with a median age of 9 (range,2-14) years old.The median follow-up was 31months.Patients with a positive NPM-ALK expression in BM had a 3-years EFS of (35.6± 18.6)％,compared with (91.7±8.0)％ for patients with negative NPM-ALK (P=0.038).The incidence of positive expression in BM was significantly higher in patients who had more than 3 organs involved by tumor (P=0.032).86.7％patients had a concordant results of NPM-ALK expression in PB and BM.Conclusion We could evaluate the minimal disseminated disease of NPM-ALK positive ALCL patients by screening the NPM-ALK fusion gene in BM and PB by RT-PCR.The positive expression is associated with a poor prognosis and could be used for stratification of ALCL.