CAJ | 학술논문

目的检测miR-203在儿童急性白血病患者中的甲基化状态及表达情况,探讨其临床意义.方法应用甲基化特异性聚合酶链反应检测miR-203启动子区CpG岛的甲基化状态,用实时荧光定量聚合酶链反应检测miR-203的相对表达量,分析其与临床指标间的关系.结果 31例儿童急性淋巴细胞白血病(ALL)、15例儿童急性髓系白血病(AML)及23份非恶性血液病患儿骨髓标本(对照组)均未检测到miR-203的甲基化现象.miR-203在对照组、儿童急性白血病组、ALL组及AML组的相对表达量分别为16.93±6.31、48.97±10.38、55.88±12.91、24.28±9.10,儿童急性白血病组、ALL组与对照组相比,差异有统计学意义(P值分别为0.011和0.009),儿童AML组与对照组相比,差异无统计学意义(P=0.514).各项临床指标中,miR-203的表达与初诊ALL患儿的性别、免疫分型、染色体核型、是否检出融合基因、BCR-ABL基因表达、SIL-TAL1基因表达、泼尼松试验及急性白血病患儿的性别、染色体核型、是否检出融合基因、SIL-TAL1基因表达相关(P值均＜0.05).其中在ALL危险度分组两两比较中,中危组与高危组两组间miR-203的表达差异有统计学意义(P=0.022).结论 miR-203在大部分急性白血病患儿中不受甲基化机制调控.miR-203可能作为原癌基因参与儿童急性白血病尤其是ALL的形成.miR-203的高表达可能与急性白血病患儿尤其是ALL患儿的不良预后相关.
목적 검측miR-203재인동급성백혈병환자중적갑기화상태급표체정황,탐토기림상의의.방법 응용갑기화특이성취합매련반응검측miR-203계동자구CpG도적갑기화상태,용실시형광정량취합매련반응검측miR-203적상대표체량,분석기여림상지표간적관계.결과 31례인동급성림파세포백혈병(ALL)、15례인동급성수계백혈병(AML)급23빈비악성혈액병환인골수표본(대조조)균미검측도miR-203적갑기화현상.miR-203재대조조、인동급성백혈병조、ALL조급AML조적상대표체량분별위16.93±6.31、48.97±10.38、55.88±12.91、24.28±9.10,인동급성백혈병조、ALL조여대조조상비,차이유통계학의의(P치분별위0.011화0.009),인동AML조여대조조상비,차이무통계학의의(P=0.514).각항림상지표중,miR-203적표체여초진ALL환인적성별、면역분형、염색체핵형、시부검출융합기인、BCR-ABL기인표체、SIL-TAL1기인표체、발니송시험급급성백혈병환인적성별、염색체핵형、시부검출융합기인、SIL-TAL1기인표체상관(P치균＜0.05).기중재ALL위험도분조량량비교중,중위조여고위조량조간miR-203적표체차이유통계학의의(P=0.022).결론 miR-203재대부분급성백혈병환인중불수갑기화궤제조공.miR-203가능작위원암기인삼여인동급성백혈병우기시ALL적형성.miR-203적고표체가능여급성백혈병환인우기시ALL환인적불량예후상관.
Objective To investigate the methylation,expression and clinical significance of miR-203 in pediatric acute leukemia.Methods The methylation status of miR-203 promoter CpG islands was detected with methylation-specific polymerase chain reaction.The expression of miR-203 was detected by Taqman real-time quantitative polymerase chain reaction.And the clinical significance of miR-203 in pediatric acute leukemia (ALL) was also analyzed.Results The promoter of miR-203 was unmethylated in all of 31 pediatric acute lymphoblastic leukemia,all of 15 pediatric acute myeloid leukemia (AML) and all of 23 controls.The relative expression levels of miR-203 in controls,pediatric acute leukemia,ALL and AML were 16.93±6.31,48.97±10.38,55.88±12.91,24.28±9.10 respectively.The results indicated that miR-203 was significantly up-regulated in pediatric acute leukemia (P=0.011) and ALL (P=0.009),not in pediatric AML (P=0.514) compared with control.The expression ofmiR-203 was significantly related with the gender,immunophenotype,chromosome,fusion gene,BCR-ABL,SIL-TALl and prednisone experiment in pediatric ALL and the gender,chromosome,fusion gene,SIL-TALl in pediatric acute leukemia (P＜0.05).And in risk stratification pairwise comparisons,the expression of miR-203 in the medium-risk and high-risk groups appeared significantly different (P=0.022).Conclusions miR-203 may not be regulated with methylation mechanism in pediatric acute leukemia,miR-203 may be a protooncogene involved in the formation of pediatric acute leukemia and ALL.Further analyses indicated that high expression of miR-203 may be associated with poor prognosis of pediatric ALL and acute leukemia.