CAJ | 학술논문

目的研究肥大细胞(MC)及IL-9在B细胞非霍奇金淋巴瘤(B-NHL)中的临床意义.方法采用Western blot法检测CD117在B-NHL患者肿瘤组织中的表达水平,应用免疫组织化学染色观察MC在B-NHL患者肿瘤局部的浸润情况.通过计算MC与B-NHL患者肿瘤标志物间的相关系数来判断MC在预后中的意义.通过ELISA方法检测B-NHL患者外周血中IL-9水平.应用流式细胞仪检测小鼠骨髓衍化的MC(BMMC)的纯度,并应用RT-PCR检测IL-9对小鼠MC相关基因表达的影响.结果 B-NHL患者肿瘤组织CDl17表达明显高于对照组(0.0551 ±0.0064对0.0192±0.0072,P＜0.0l),局部浸润的MC明显增多,且与患者预后不良相关;B-NHL患者血清中IL-9水平明显高于健康对照组;体外实验证实IL-9可以诱导小鼠BMMC的分化及相关基因的表达,包括CDll7、Fcerlα、Mcpt1和Mcpt5.结论 IL-9可增强MC介导的免疫反应,在促进肿瘤的生长中可能起重要作用.IL-9有可能成为B-NHL的免疫治疗的作用靶点.
목적 연구비대세포(MC)급IL-9재B세포비곽기금림파류(B-NHL)중적림상의의.방법 채용Western blot법검측CD117재B-NHL환자종류조직중적표체수평,응용면역조직화학염색관찰MC재B-NHL환자종류국부적침윤정황.통과계산MC여B-NHL환자종류표지물간적상관계수래판단MC재예후중적의의.통과ELISA방법검측B-NHL환자외주혈중IL-9수평.응용류식세포의검측소서골수연화적MC(BMMC)적순도,병응용RT-PCR검측IL-9대소서MC상관기인표체적영향.결과 B-NHL환자종류조직CDl17표체명현고우대조조(0.0551 ±0.0064대0.0192±0.0072,P＜0.0l),국부침윤적MC명현증다,차여환자예후불량상관;B-NHL환자혈청중IL-9수평명현고우건강대조조;체외실험증실IL-9가이유도소서BMMC적분화급상관기인적표체,포괄CDll7、Fcerlα、Mcpt1화Mcpt5.결론 IL-9가증강MC개도적면역반응,재촉진종류적생장중가능기중요작용.IL-9유가능성위B-NHL적면역치료적작용파점.
Objective To investigate the role of mast cells and interleukin-9 (IL-9) in B-cell non-Hodgkin lymphoma (B-NHL) development and its clinical significance.Methods The expression level of CD117 in tumor tissues of 32 B-NHL patients was determined by Western blot.The infiltration of CDll7+mast cells (MCs) in human B-NHL tumor tissues was observed by immunohistochemistry staining.To evaluate the correlations between the data from CD117+ MCs and biological markers of human B-NHL,a Spearman correlation coefficient (rs) was calculated.IL-9 levels in sera of B-NHL patients were measured by ELISA.Effects of IL-9 on expressions of functional genes of mouse bone marrow-derived mast cells (BMMCs) were detected by real-time quantitative RT-PCR.Results The expression of CD117 was up-regulated significantly in human B-cell NHL involved tissues when compared with that of controls (0.0551±0.0064 vs 0.0192±0.0072,P＜0.01).Infiltration of more CD117+ MCs was found in tissues from B-cell NHL subjects compared with that of controls.IL-9 level in serum samples from patients with B-cell NHL was higher than that from healthy controls.Addition of rlL-9 to the culture gave rise to increase in the purity of mouse BMMCs in the first three weeks.In vitro culture experiments showed that the addition of IL-9 could induce the differentiation of mouse BMMC and the expressions of MC-related genes,including CD117,Fcerlα,Mcpt1 and Mcpt5.Conclusion Our study showed that IL-9 promoted immune response mediated by MCs,and probably played important roles in B-NHL growth.Pharmacological or targeted inhibition of mast cells or IL-9 activity may provide new strategy for B-cell NHL therapy.