中华眼底病杂志
中華眼底病雜誌
중화안저병잡지
CHINESE JOURNAL OF OCULAR FUNDUS DISEASES
2013年
3期
300-304
,共5页
常花蕾%杨新光%于敬妮%齐赟
常花蕾%楊新光%于敬妮%齊赟
상화뢰%양신광%우경니%제빈
再灌注损伤/药物疗法%秋水仙属/药物作用%肿瘤坏死因子α%白细胞介素-1β%动物实验
再灌註損傷/藥物療法%鞦水仙屬/藥物作用%腫瘤壞死因子α%白細胞介素-1β%動物實驗
재관주손상/약물요법%추수선속/약물작용%종류배사인자α%백세포개소-1β%동물실험
Reperfusion injury/drug therapy%Colchicum/drug effects%Tumor necrosis factor alpha%Interleukin-1beta%Animal experimentation
目的 观察藏红花素对视网膜缺血再灌注损伤(RIR)大鼠视网膜组织结构及肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)表达的影响.方法 采用随机数字表法将80只8~10周龄健康无眼疾Sprague-Dawley(SD)雄性大鼠随机分为正常对照组、模型组、藏红花素低剂量组和藏红花素高剂量组,每组20只.正常对照组大鼠不作任何处理.其余组采用前房灌注生理盐水升高眼内压法建立RIR模型.藏红花素低剂量组、藏红花素高剂量组均于建模前30 min和建模后每天定时分别以5mg/kg、50mg/kg的剂量腹腔注射藏红花素溶液.建模后6、12、24、48 h,作视网膜石蜡切片行苏木精-伊红染色,光学显微镜观察各组大鼠视网膜组织结构;作视网膜组织匀浆,采用酶联免疫吸附试验测定各组TNF-α、IL-1β的表达.结果 光学显微镜观察发现,正常对照组大鼠视网膜组织结构正常;模型组大鼠出现视网膜水肿、结构紊乱、细胞排列疏松等病理改变;藏红花素低剂量组大鼠视网膜病理改变程度较模型组轻;藏红花素高剂量组大鼠视网膜组织结构与正常对照组相似.TNF-α在建模后24 h表达量最高,IL-1β在建模后12、48 h时表达量最高.建模后6、12、24、48 h,模型组(t=5.42、7.94、9.32、9.18)、藏红花素低剂量组(t=3.94、4.12、4.98、3.84)TNF-α表达均较正常对照组升高,差异有统计学意义(P<0.05);藏红花素高剂量组TNF-α表达较正常对照组有所升高,但差异无统计学意义(t=2.13、2.34、2.96、2.78,P>0.05).与模型组比较,藏红花素低剂量组(t=3.95、4.56、4.01、5.12)、藏红花素高剂量组(t=5.23、7.65、7.74、7.63)TNF-α表达均降低,差异有统计学意义(P<0.05).建模后6、12、24、48 h,模型组(t=7.23、7.87、7.15、15.60)、藏红花素低剂量组(t=5.65、5.10、5.54、6.87)、藏红花素高剂量组(t=4.38、5.21、4.56、4.75)IL-1β表达均较正常对照组升高,差异有统计学意义(P<0.05).与模型组比较,藏红花素低剂量组仅于建模后48 h降低最为明显,差异有统计学意义(t=7.56,P<0.05);各时间点藏红花素高剂量组IL-1β表达均降低,差异均有统计学意义(t=6.94、5.36、6.05、10.50,P<0.05).结论 藏红花素可以改善RIR大鼠视网膜组织结构的病理改变,降低TNF-α、IL-1β的表达.
目的 觀察藏紅花素對視網膜缺血再灌註損傷(RIR)大鼠視網膜組織結構及腫瘤壞死因子-α(TNF-α)、白細胞介素-1β(IL-1β)錶達的影響.方法 採用隨機數字錶法將80隻8~10週齡健康無眼疾Sprague-Dawley(SD)雄性大鼠隨機分為正常對照組、模型組、藏紅花素低劑量組和藏紅花素高劑量組,每組20隻.正常對照組大鼠不作任何處理.其餘組採用前房灌註生理鹽水升高眼內壓法建立RIR模型.藏紅花素低劑量組、藏紅花素高劑量組均于建模前30 min和建模後每天定時分彆以5mg/kg、50mg/kg的劑量腹腔註射藏紅花素溶液.建模後6、12、24、48 h,作視網膜石蠟切片行囌木精-伊紅染色,光學顯微鏡觀察各組大鼠視網膜組織結構;作視網膜組織勻漿,採用酶聯免疫吸附試驗測定各組TNF-α、IL-1β的錶達.結果 光學顯微鏡觀察髮現,正常對照組大鼠視網膜組織結構正常;模型組大鼠齣現視網膜水腫、結構紊亂、細胞排列疏鬆等病理改變;藏紅花素低劑量組大鼠視網膜病理改變程度較模型組輕;藏紅花素高劑量組大鼠視網膜組織結構與正常對照組相似.TNF-α在建模後24 h錶達量最高,IL-1β在建模後12、48 h時錶達量最高.建模後6、12、24、48 h,模型組(t=5.42、7.94、9.32、9.18)、藏紅花素低劑量組(t=3.94、4.12、4.98、3.84)TNF-α錶達均較正常對照組升高,差異有統計學意義(P<0.05);藏紅花素高劑量組TNF-α錶達較正常對照組有所升高,但差異無統計學意義(t=2.13、2.34、2.96、2.78,P>0.05).與模型組比較,藏紅花素低劑量組(t=3.95、4.56、4.01、5.12)、藏紅花素高劑量組(t=5.23、7.65、7.74、7.63)TNF-α錶達均降低,差異有統計學意義(P<0.05).建模後6、12、24、48 h,模型組(t=7.23、7.87、7.15、15.60)、藏紅花素低劑量組(t=5.65、5.10、5.54、6.87)、藏紅花素高劑量組(t=4.38、5.21、4.56、4.75)IL-1β錶達均較正常對照組升高,差異有統計學意義(P<0.05).與模型組比較,藏紅花素低劑量組僅于建模後48 h降低最為明顯,差異有統計學意義(t=7.56,P<0.05);各時間點藏紅花素高劑量組IL-1β錶達均降低,差異均有統計學意義(t=6.94、5.36、6.05、10.50,P<0.05).結論 藏紅花素可以改善RIR大鼠視網膜組織結構的病理改變,降低TNF-α、IL-1β的錶達.
목적 관찰장홍화소대시망막결혈재관주손상(RIR)대서시망막조직결구급종류배사인자-α(TNF-α)、백세포개소-1β(IL-1β)표체적영향.방법 채용수궤수자표법장80지8~10주령건강무안질Sprague-Dawley(SD)웅성대서수궤분위정상대조조、모형조、장홍화소저제량조화장홍화소고제량조,매조20지.정상대조조대서불작임하처리.기여조채용전방관주생리염수승고안내압법건립RIR모형.장홍화소저제량조、장홍화소고제량조균우건모전30 min화건모후매천정시분별이5mg/kg、50mg/kg적제량복강주사장홍화소용액.건모후6、12、24、48 h,작시망막석사절편행소목정-이홍염색,광학현미경관찰각조대서시망막조직결구;작시망막조직균장,채용매련면역흡부시험측정각조TNF-α、IL-1β적표체.결과 광학현미경관찰발현,정상대조조대서시망막조직결구정상;모형조대서출현시망막수종、결구문란、세포배렬소송등병리개변;장홍화소저제량조대서시망막병리개변정도교모형조경;장홍화소고제량조대서시망막조직결구여정상대조조상사.TNF-α재건모후24 h표체량최고,IL-1β재건모후12、48 h시표체량최고.건모후6、12、24、48 h,모형조(t=5.42、7.94、9.32、9.18)、장홍화소저제량조(t=3.94、4.12、4.98、3.84)TNF-α표체균교정상대조조승고,차이유통계학의의(P<0.05);장홍화소고제량조TNF-α표체교정상대조조유소승고,단차이무통계학의의(t=2.13、2.34、2.96、2.78,P>0.05).여모형조비교,장홍화소저제량조(t=3.95、4.56、4.01、5.12)、장홍화소고제량조(t=5.23、7.65、7.74、7.63)TNF-α표체균강저,차이유통계학의의(P<0.05).건모후6、12、24、48 h,모형조(t=7.23、7.87、7.15、15.60)、장홍화소저제량조(t=5.65、5.10、5.54、6.87)、장홍화소고제량조(t=4.38、5.21、4.56、4.75)IL-1β표체균교정상대조조승고,차이유통계학의의(P<0.05).여모형조비교,장홍화소저제량조부우건모후48 h강저최위명현,차이유통계학의의(t=7.56,P<0.05);각시간점장홍화소고제량조IL-1β표체균강저,차이균유통계학의의(t=6.94、5.36、6.05、10.50,P<0.05).결론 장홍화소가이개선RIR대서시망막조직결구적병리개변,강저TNF-α、IL-1β적표체.
Objective To observe the effect of Crocin on structure and the expression of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) in rat retina after injury by ischemia-reperfusion.Methods A total of 80 Sprague-Dawley male rats at the age of 8-10 weeks were divided into control group,model group,low-dose Crocin group and high-dose Crocin group,with 20 rats in each group.The rats of control group were not treated.The rats in model,low-dose Crocin and high-dose Crocin group were induced with normal saline by anterior chamber perfusion creating a retinal ischemia-reperfusion (RIR) model.The rats of the low-dose Crocin and high-dose Crocin group received intraperitoneal injection with different doses of Crocin solution (5 mg/kg,or 50 mg/kg) 30 minutes prior to ischemic injury and one time per day after successful RIR.Optical microscopy was used to observe the retinal structure.Enzyme-linked immunosorbent assay (ELISA) was used to measure the expression of TNF-α and IL-1β 6,12,24 and 48 hours after RIR.Results The retinal structure of control group was normal.Pathological changes were found in the RIR model and low-dose Crocin group,such as retinal edema,disorganized structure and loosely packed cells.The degree of pathological changes in low dose Crocin group was less than the RIR model group.The retinal structure of high-dose Crocin group was similar to the control group.The expression of TNF-α was the highest at 24 hours after modeling,while the expression of IL-1β was the highest at 12 and 48 hours after RIR modeling.Six,12,24 and 48 hours after RIR modeling,compared with the control group,the TNF-α expression of model (t=5.42,7.94,9.32,9.18; P<0.05),low-dose Crocin (t=3.94,4.12,4.98,3.84; P<0.05) and high-dose Crocin group (t=2.13,2.34,2.96,2.78;P>0.05) were increased.Compared with the RIR model group,the TNF-α expression of low-dose Crocin (t=3.95,4.56,4.01,5.12) and high-dose Crocin group (t =5.23,7.65,7.74,7.63) was decreased.Compared with the control group,the IL-1β expression of model (t=7.23,7.87,7.15,15.60),low-dose Crocin (t 5.65,5.10,5.54,6.87;P<0.05) and high-dose Crocin group (t=4.38,5.21,4.56,4.75) was increased (P<0.05).Compared with the model group,the IL-1β expression of low-dose Crocin group was decreased significantly 48 hours after RIR modeling (t=7.56,P<0.05) ; but it decreased significantly at each time point in high-dose Crocin group (t=6.94,5.36,6.05,10.50; P<0.05).Conclusion Crocin can improve the retinal pathologic changes,while down regulating TNF-α and IL-1β expression in RIR rats.